LIS1_CANAW
ID LIS1_CANAW Reviewed; 486 AA.
AC C4YPI7;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Nuclear distribution protein PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Lissencephaly-1 homolog {ECO:0000255|HAMAP-Rule:MF_03141};
DE Short=LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=nudF homolog {ECO:0000255|HAMAP-Rule:MF_03141};
GN Name=PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN Synonyms=LIS1 {ECO:0000255|HAMAP-Rule:MF_03141}; ORFNames=CAWG_02388;
OS Candida albicans (strain WO-1) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=294748;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WO-1;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC microtubule motor protein dynein. Plays a central role in positioning
CC the mitotic spindle at the bud neck during cell division. Targets
CC cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-
CC mediated microtubule sliding along the bud cortex and consequently the
CC movement of the mitotic spindle to the bud neck. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SUBUNIT: Self-associates. Interacts with NDL1 and dynein.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC Rule:MF_03141}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the plus ends of
CC microtubules and the mitotic spindle poles. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
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DR EMBL; CM000310; EEQ44126.1; -; Genomic_DNA.
DR AlphaFoldDB; C4YPI7; -.
DR SMR; C4YPI7; -.
DR STRING; 5476.C4YPI7; -.
DR EnsemblFungi; EEQ44126; EEQ44126; CAWG_02388.
DR VEuPathDB; FungiDB:CAWG_02388; -.
DR HOGENOM; CLU_000288_57_15_1; -.
DR OMA; TQECKCV; -.
DR Proteomes; UP000001429; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005875; C:microtubule associated complex; IEA:UniProtKB-UniRule.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0070840; F:dynein complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:UniProtKB-UniRule.
DR GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03141; lis1; 1.
DR InterPro; IPR017252; Dynein_regulator_LIS1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037190; LIS1_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 5.
DR PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF109925; SSF109925; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Repeat; Transport; WD repeat.
FT CHAIN 1..486
FT /note="Nuclear distribution protein PAC1"
FT /id="PRO_0000405072"
FT REPEAT 119..158
FT /note="WD 1"
FT REPEAT 164..205
FT /note="WD 2"
FT REPEAT 206..246
FT /note="WD 3"
FT REPEAT 249..291
FT /note="WD 4"
FT REPEAT 294..328
FT /note="WD 5"
FT REPEAT 329..368
FT /note="WD 6"
FT REPEAT 389..428
FT /note="WD 7"
FT REPEAT 437..483
FT /note="WD 8"
FT COILED 66..99
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
SQ SEQUENCE 486 AA; 55358 MW; 166A0B449BF8A104 CRC64;
MEKLQILTER QQTELNHAII QYLQPLCQQD NHVLLDQLSK LLNIDQSTQE SNNVEKVDNY
LEKRWSTVLR LQKKIIDLEN EISNLNNIIN STNSDNNGII LSKDKINWIP KGAVKQSYQC
ENIVTTVKLH PNLPLVLNGC NDGNLYIWNI SNDDNTIPEK MIKAHTRAIN KICFTYKKPY
YLATCSSDLT IKIWDEKFNH IRTLNGHEHT VSSIQFSPVD NSILYSVSRD KNIRVWDIFQ
GISLKSFVGH SEWCRDLDII SSDTYGDFVL TCSNDQSARL SHANSGAGVA MIVGHSHVVE
TVKFLPSLQA NKILDEYITK NTEQFPTIPL ELLKDKTYNQ LGFKYCITAS RDNTIKLWLI
PPPTIAPHRP PLPSKYNNSQ SWLIAELKGH SSWVKSLCVH PNGKFIISGS DDKTIKFWDL
SGLLETGYVN VVKTIIGHDG FINDIDFARL KEASDVSEED LLKQVEKRMR CLFISGSADN
SIKLWN
