LIS1_CANDC
ID LIS1_CANDC Reviewed; 489 AA.
AC B9WD30;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Nuclear distribution protein PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Lissencephaly-1 homolog {ECO:0000255|HAMAP-Rule:MF_03141};
DE Short=LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=nudF homolog {ECO:0000255|HAMAP-Rule:MF_03141};
GN Name=PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN Synonyms=LIS1 {ECO:0000255|HAMAP-Rule:MF_03141}; ORFNames=CD36_80490;
OS Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 /
OS NRRL Y-17841) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=573826;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841;
RX PubMed=19745113; DOI=10.1101/gr.097501.109;
RA Jackson A.P., Gamble J.A., Yeomans T., Moran G.P., Saunders D., Harris D.,
RA Aslett M., Barrell J.F., Butler G., Citiulo F., Coleman D.C.,
RA de Groot P.W.J., Goodwin T.J., Quail M.A., McQuillan J., Munro C.A.,
RA Pain A., Poulter R.T., Rajandream M.A., Renauld H., Spiering M.J.,
RA Tivey A., Gow N.A.R., Barrell B., Sullivan D.J., Berriman M.;
RT "Comparative genomics of the fungal pathogens Candida dubliniensis and
RT Candida albicans.";
RL Genome Res. 19:2231-2244(2009).
CC -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC microtubule motor protein dynein. Plays a central role in positioning
CC the mitotic spindle at the bud neck during cell division. Targets
CC cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-
CC mediated microtubule sliding along the bud cortex and consequently the
CC movement of the mitotic spindle to the bud neck. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SUBUNIT: Self-associates. Interacts with NDL1 and dynein.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC Rule:MF_03141}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the plus ends of
CC microtubules and the mitotic spindle poles. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
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DR EMBL; FM992690; CAX42579.1; -; Genomic_DNA.
DR RefSeq; XP_002418997.1; XM_002418952.1.
DR AlphaFoldDB; B9WD30; -.
DR SMR; B9WD30; -.
DR STRING; 42374.XP_002418997.1; -.
DR EnsemblFungi; CAX42579; CAX42579; CD36_80490.
DR GeneID; 8046779; -.
DR KEGG; cdu:CD36_80490; -.
DR CGD; CAL0000161252; Cd36_80490.
DR eggNOG; KOG0295; Eukaryota.
DR HOGENOM; CLU_000288_57_15_1; -.
DR OrthoDB; 995692at2759; -.
DR Proteomes; UP000002605; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005875; C:microtubule associated complex; IEA:UniProtKB-UniRule.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0070840; F:dynein complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:UniProtKB-UniRule.
DR GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03141; lis1; 1.
DR InterPro; IPR017252; Dynein_regulator_LIS1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037190; LIS1_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 5.
DR PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF109925; SSF109925; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Repeat; Transport; WD repeat.
FT CHAIN 1..489
FT /note="Nuclear distribution protein PAC1"
FT /id="PRO_0000405073"
FT REPEAT 119..158
FT /note="WD 1"
FT REPEAT 164..205
FT /note="WD 2"
FT REPEAT 206..246
FT /note="WD 3"
FT REPEAT 249..291
FT /note="WD 4"
FT REPEAT 328..368
FT /note="WD 5"
FT REPEAT 389..428
FT /note="WD 6"
FT REPEAT 437..486
FT /note="WD 7"
FT COILED 66..98
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
SQ SEQUENCE 489 AA; 55663 MW; 040DA233F741574F CRC64;
MEKLSILTER QQTELNYAII QYLQPLCQQD NHALLDQLSK ILNIDQWTQE SNNVEKVDNY
LEKRWSTVLR LQKKIIDLEN EISNLNNIIN SSNSDNNGIV LSKDKINWIP KGTAKQSYQC
ENIVTTVKLH PNLPLVLNGC NDGNLYIWNI SNDDNTIPEK MIKAHTRAIN KICFTYKKPY
YLATCSSDLT IKIWDEKFNH IRTLNGHEHT VSSIQFSPID NSILYSVSRD KNIRVWDIFQ
GISLKSFVGH SEWCRDLDIV SSDNNGDFVL TCSNDQSARL SHASSGAGLA MIVGHGHVVE
TVKFLPALQA NKILDEYITK NIEQFPTIPL ELLKDKTYNQ LGFKYCITAS RDNTIKLWLI
PPPKIAPHRP PLPSKYNNSQ SWMIAELRGH SSWVKCLCVH PNGRFIISGS DDKTIKFWDL
SSLLETGSVN VVKTIIGHDG FINDIDFARL KEASDSTPTS QEDLLKEVEK RMRCLFISGS
ADNSIKLWN
