LIS1_CANTT
ID LIS1_CANTT Reviewed; 490 AA.
AC C5MJE8;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Nuclear distribution protein PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Lissencephaly-1 homolog {ECO:0000255|HAMAP-Rule:MF_03141};
DE Short=LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=nudF homolog {ECO:0000255|HAMAP-Rule:MF_03141};
GN Name=PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN Synonyms=LIS1 {ECO:0000255|HAMAP-Rule:MF_03141}; ORFNames=CTRG_06191;
OS Candida tropicalis (strain ATCC MYA-3404 / T1) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=294747;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-3404 / T1;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC microtubule motor protein dynein. Plays a central role in positioning
CC the mitotic spindle at the bud neck during cell division. Targets
CC cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-
CC mediated microtubule sliding along the bud cortex and consequently the
CC movement of the mitotic spindle to the bud neck. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SUBUNIT: Self-associates. Interacts with NDL1 and dynein.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC Rule:MF_03141}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the plus ends of
CC microtubules and the mitotic spindle poles. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
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DR EMBL; GG692406; EER30151.1; -; Genomic_DNA.
DR RefSeq; XP_002546713.1; XM_002546667.1.
DR AlphaFoldDB; C5MJE8; -.
DR SMR; C5MJE8; -.
DR STRING; 5482.XP_002546713.1; -.
DR EnsemblFungi; EER30151; EER30151; CTRG_06191.
DR GeneID; 8300052; -.
DR KEGG; ctp:CTRG_06191; -.
DR VEuPathDB; FungiDB:CTRG_06191; -.
DR eggNOG; KOG0295; Eukaryota.
DR OrthoDB; 995692at2759; -.
DR Proteomes; UP000002037; Unassembled WGS sequence.
DR GO; GO:0005881; C:cytoplasmic microtubule; IEA:EnsemblFungi.
DR GO; GO:0005875; C:microtubule associated complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:EnsemblFungi.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0070840; F:dynein complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:UniProtKB-UniRule.
DR GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR GO; GO:0030473; P:nuclear migration along microtubule; IEA:EnsemblFungi.
DR GO; GO:1903033; P:positive regulation of microtubule plus-end binding; IEA:EnsemblFungi.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03141; lis1; 1.
DR InterPro; IPR017252; Dynein_regulator_LIS1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037190; LIS1_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 5.
DR PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF109925; SSF109925; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..490
FT /note="Nuclear distribution protein PAC1"
FT /id="PRO_0000405075"
FT REPEAT 118..157
FT /note="WD 1"
FT REPEAT 163..204
FT /note="WD 2"
FT REPEAT 205..245
FT /note="WD 3"
FT REPEAT 251..290
FT /note="WD 4"
FT REPEAT 293..327
FT /note="WD 5"
FT REPEAT 328..367
FT /note="WD 6"
FT REPEAT 388..427
FT /note="WD 7"
FT REPEAT 436..487
FT /note="WD 8"
FT COILED 65..96
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
SQ SEQUENCE 490 AA; 55618 MW; D5AA21D6B17108B7 CRC64;
MMERSQILTE RQQSELNKAI IQYLQPICSQ ENNEVLDKLT SMLKIESTEL DGSDIVDNYL
EKKWSTVLRL QKKIIDLENE IHNLTNIINT TNSETNGVVL SKDKINWIPK GASKQTYQCE
NVVATVRLHP NLPLVFNGCN DGNLYIWNLT NDDNTIPEKR IKAHTRSINK MCFSYRKPYY
LATCSSDLTI KIWDEKFNHI RTLNGHEHTV SSVKFSPSDS NILYSVSRDK NIRVWDISQG
VCLKSFVGHS EWCRDLDAVA SETQGDFVLT CSNDQSARLS HINSGVGVAM FVGHTHVVES
VKFLPKIQAN ELIDEYITKN IDQFPSIPSE LLKDPIYDEL GFKYCVSASR DNTIKLWLIP
PPTLIPHRSP LPSKYNNSQG WLIAEFKGHS SWVKCLSVHP NGKFIISGSD DKTIKFWDLS
GLIETGSVTA IKTISGHEGF INDIDFARLT DSESNTDKEL TSEEYLKDVE KRMRCLFISG
SADNSIKLWS
