ID   LIS1_KOMPG              Reviewed;         500 AA.
AC   C4R6H3;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Nuclear distribution protein PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE   AltName: Full=Lissencephaly-1 homolog {ECO:0000255|HAMAP-Rule:MF_03141};
DE            Short=LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE   AltName: Full=nudF homolog {ECO:0000255|HAMAP-Rule:MF_03141};
GN   Name=PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN   Synonyms=LIS1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN   OrderedLocusNames=PAS_chr3_1092;
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864;
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC       microtubule motor protein dynein. Plays a central role in positioning
CC       the mitotic spindle at the bud neck during cell division. Targets
CC       cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-
CC       mediated microtubule sliding along the bud cortex and consequently the
CC       movement of the mitotic spindle to the bud neck. {ECO:0000255|HAMAP-
CC       Rule:MF_03141}.
CC   -!- SUBUNIT: Self-associates. Interacts with NDL1 and dynein.
CC       {ECO:0000255|HAMAP-Rule:MF_03141}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC       Rule:MF_03141}. Cytoplasm, cytoskeleton, spindle pole
CC       {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the plus ends of
CC       microtubules and the mitotic spindle poles. {ECO:0000255|HAMAP-
CC       Rule:MF_03141}.
CC   -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC       {ECO:0000255|HAMAP-Rule:MF_03141}.
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DR   EMBL; FN392321; CAY71159.1; -; Genomic_DNA.
DR   RefSeq; XP_002493338.1; XM_002493293.1.
DR   AlphaFoldDB; C4R6H3; -.
DR   SMR; C4R6H3; -.
DR   STRING; 644223.C4R6H3; -.
DR   EnsemblFungi; CAY71159; CAY71159; PAS_chr3_1092.
DR   GeneID; 8200051; -.
DR   KEGG; ppa:PAS_chr3_1092; -.
DR   eggNOG; KOG0295; Eukaryota.
DR   HOGENOM; CLU_000288_57_15_1; -.
DR   InParanoid; C4R6H3; -.
DR   OMA; TQECKCV; -.
DR   Proteomes; UP000000314; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005875; C:microtubule associated complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR   GO; GO:0070840; F:dynein complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:UniProtKB-UniRule.
DR   GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 2.
DR   HAMAP; MF_03141; lis1; 1.
DR   InterPro; IPR017252; Dynein_regulator_LIS1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR037190; LIS1_N.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 4.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF109925; SSF109925; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 4.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Microtubule; Mitosis; Reference proteome; Repeat; Transport; WD repeat.
FT   CHAIN           1..500
FT                   /note="Nuclear distribution protein PAC1"
FT                   /id="PRO_0000405094"
FT   REPEAT          125..164
FT                   /note="WD 1"
FT   REPEAT          169..219
FT                   /note="WD 2"
FT   REPEAT          225..265
FT                   /note="WD 3"
FT   REPEAT          268..310
FT                   /note="WD 4"
FT   REPEAT          338..378
FT                   /note="WD 5"
FT   REPEAT          397..436
FT                   /note="WD 6"
FT   REPEAT          459..500
FT                   /note="WD 7"
SQ   SEQUENCE   500 AA;  56916 MW;  404F758C68208D7A CRC64;
     MSDILTKKQD IELRRAVIQY LRYFVPEFAQ DTAVNTISRL LLDADLNKFD NEIQRMIPKQ
     YLEKKWTAVL RLHRKVEELE RTIGYQSNKV EIELQSQPHS DITSNSLDRI NWVPGIEPFQ
     KLNMHNGHPV TAIDIHPFQP IMATASQDGT IVIWNLLNLT EPQQIIRNAH TRSINTIKFM
     NQEVMDGHST KKNRLLLATG SSDLLIKIWD IEDSANLKAL RTLTGHENII SCLCFHPTEP
     SKLVSCSKDK TTKVWDTRTG SIVLSFVGHS NWVRSVDINR TGEFTLTSSN DHSIRLSSLL
     TGTGIGLMIG HEQVVEKVKF LPMACNKYLD HIAGVKFQNE LKINDDNYNK VDYKYCISGG
     RDNSVCIWLL PLPLIRSDGT MHPSTNPEGK LILKLTEHKS WVKDIAIHPN SRFIISVGDD
     RKINIWDLGL LLESNLLQPF RSISTQGFPS TLCMAKPVIK DQESDDLILL NEAMRCYLAV
     GHSDGTVTLW KKNIQKEYIL