LIS1_PICST
ID LIS1_PICST Reviewed; 524 AA.
AC A3LNI7;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Nuclear distribution protein PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Lissencephaly-1 homolog {ECO:0000255|HAMAP-Rule:MF_03141};
DE Short=LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=nudF homolog {ECO:0000255|HAMAP-Rule:MF_03141};
GN Name=PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN Synonyms=LIS1 {ECO:0000255|HAMAP-Rule:MF_03141}; ORFNames=PICST_76411;
OS Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS Y-11545) (Yeast) (Pichia stipitis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX NCBI_TaxID=322104;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX PubMed=17334359; DOI=10.1038/nbt1290;
RA Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA Passoth V., Richardson P.M.;
RT "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT yeast Pichia stipitis.";
RL Nat. Biotechnol. 25:319-326(2007).
CC -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC microtubule motor protein dynein. Plays a central role in positioning
CC the mitotic spindle at the bud neck during cell division. Targets
CC cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-
CC mediated microtubule sliding along the bud cortex and consequently the
CC movement of the mitotic spindle to the bud neck. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SUBUNIT: Self-associates. Interacts with NDL1 and dynein.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC Rule:MF_03141}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the plus ends of
CC microtubules and the mitotic spindle poles. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
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DR EMBL; CP000496; ABN64861.2; -; Genomic_DNA.
DR RefSeq; XP_001382890.2; XM_001382853.1.
DR AlphaFoldDB; A3LNI7; -.
DR SMR; A3LNI7; -.
DR STRING; 4924.XP_001382890.2; -.
DR EnsemblFungi; ABN64861; ABN64861; PICST_76411.
DR GeneID; 4836636; -.
DR KEGG; pic:PICST_76411; -.
DR eggNOG; KOG0295; Eukaryota.
DR HOGENOM; CLU_000288_57_15_1; -.
DR InParanoid; A3LNI7; -.
DR OMA; TQECKCV; -.
DR OrthoDB; 995692at2759; -.
DR Proteomes; UP000002258; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005875; C:microtubule associated complex; IEA:UniProtKB-UniRule.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0070840; F:dynein complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:UniProtKB-UniRule.
DR GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03141; lis1; 1.
DR InterPro; IPR017252; Dynein_regulator_LIS1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037190; LIS1_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 3.
DR PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF109925; SSF109925; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..524
FT /note="Nuclear distribution protein PAC1"
FT /id="PRO_0000405095"
FT REPEAT 121..160
FT /note="WD 1"
FT REPEAT 166..217
FT /note="WD 2"
FT REPEAT 220..262
FT /note="WD 3"
FT REPEAT 277..317
FT /note="WD 4"
FT REPEAT 353..394
FT /note="WD 5"
FT REPEAT 415..454
FT /note="WD 6"
FT REPEAT 463..492
FT /note="WD 7"
FT REPEAT 493..524
FT /note="WD 8"
FT COILED 65..90
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
SQ SEQUENCE 524 AA; 58298 MW; 94386B13EE55B8C7 CRC64;
MNRSQILTDR QQQELNKAIL QYLEPMLTES SSDQEVYRSL QQILVPPSSS SSEAIVDNYL
EKKWSTVLRL QRKIIDLENE VGTLRSIVDG QQSVSNGAAS VISKDRINWL PNRASKSFPT
QQNQLVMASV IHPVLPVIFG GCSDGSIIVW NIVNDDTSIP EKIIRAHTRS INKLAFSVEP
ADLSKDLTKS KTYIFASCSA DLSIKIWDSS SYRHIRTLTG HDHTVSSVAF SQSNPDHLYS
VSRDKTVKIW DLVNGYCVKS FTGHSEWVRD IDVASVNSQL SLNNMKVSAE LGDFVVTCSN
DQSVRVSHAE SGAGLALLIG HTHVIEKVKF LPAVSNTILD KFLKENHAQF PSIPQELVSD
EIFTTTLGYK YCISGGRDNL LKLWLIPPPT LIPHRSPLPA QHNNSQGWAI ADLVGHQSWV
KAIAIHPNGR FIFSGSDDKT IKVWDLANLN VTGSVKCVRT LSGHDGFIND LDFAAFNRDS
GKEDKIKEDA TEEESHQQLM KFIEGRMRCL FISGAADNSI KLWS
