LITE1_CAEEL
ID LITE1_CAEEL Reviewed; 439 AA.
AC Q17990; Q963E8;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 3.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=High-energy light unresponsive protein 1;
GN Name=lite-1 {ECO:0000312|WormBase:C14F11.3};
GN Synonyms=gur-2 {ECO:0000312|WormBase:C14F11.3};
GN ORFNames=C14F11.3 {ECO:0000312|WormBase:C14F11.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 13-439.
RA Robertson H.M.;
RT "Gustatory related receptors in nematodes.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=18687026; DOI=10.1371/journal.pbio.0060198;
RA Edwards S.L., Charlie N.K., Milfort M.C., Brown B.S., Gravlin C.N.,
RA Knecht J.E., Miller K.G.;
RT "A novel molecular solution for ultraviolet light detection in
RT Caenorhabditis elegans.";
RL PLoS Biol. 6:E198-E198(2008).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF SER-226; ALA-332 AND ARG-401.
RX PubMed=20436480; DOI=10.1038/nn.2540;
RA Liu J., Ward A., Gao J., Dong Y., Nishio N., Inada H., Kang L., Yu Y.,
RA Ma D., Xu T., Mori I., Xie Z., Xu X.Z.;
RT "C. elegans phototransduction requires a G protein-dependent cGMP pathway
RT and a taste receptor homolog.";
RL Nat. Neurosci. 13:715-722(2010).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, DOMAIN, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF TRP-77; SER-226; TRP-328 AND ALA-332.
RX PubMed=27863243; DOI=10.1016/j.cell.2016.10.053;
RA Gong J., Yuan Y., Ward A., Kang L., Zhang B., Wu Z., Peng J., Feng Z.,
RA Liu J., Xu X.Z.;
RT "The C. elegans taste receptor homolog LITE-1 is a photoreceptor.";
RL Cell 167:1252-1263(2016).
RN [6]
RP ERRATUM OF PUBMED:27863243.
RX PubMed=28086096; DOI=10.1016/j.cell.2016.12.040;
RA Gong J., Yuan Y., Ward A., Kang L., Zhang B., Wu Z., Peng J., Feng Z.,
RA Liu J., Xu X.Z.S.;
RT "The C. elegans taste receptor homolog LITE-1 is a photoreceptor.";
RL Cell 168:325-325(2017).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF ARG-401.
RX PubMed=29500338; DOI=10.1038/s41467-018-02934-5;
RA De Magalhaes Filho C.D., Henriquez B., Seah N.E., Evans R.M.,
RA Lapierre L.R., Dillin A.;
RT "Visible light reduces C. elegans longevity.";
RL Nat. Commun. 9:927-927(2018).
CC -!- FUNCTION: Photoreceptor for short wavelength (UV) light that mediates
CC UV-light-induced avoidance behavior (PubMed:18687026, PubMed:20436480,
CC PubMed:27863243). Directly senses and absorbs both UV-A and UV-B light
CC with very high efficiency (PubMed:27863243). Absorption of UV-B but not
CC UV-A light shows resistance to photobleaching (PubMed:27863243). In
CC contrast to other photoreceptors, does not use a prosthetic chromophore
CC to capture photons and only depends on its protein conformation
CC (PubMed:27863243). Might have a role in response to white light
CC exposure (PubMed:29500338). {ECO:0000269|PubMed:18687026,
CC ECO:0000269|PubMed:20436480, ECO:0000269|PubMed:27863243,
CC ECO:0000269|PubMed:29500338}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27863243};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the AVG and PVT neurons of the tail.
CC {ECO:0000269|PubMed:18687026}.
CC -!- DOMAIN: In contrast to other members of the family, adopts a reversed
CC membrane topology. {ECO:0000269|PubMed:27863243}.
CC -!- DISRUPTION PHENOTYPE: Worms exhibit no response to blue-violet light
CC but respond to physical stimulation. {ECO:0000269|PubMed:18687026,
CC ECO:0000269|PubMed:27863243}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to play a role in the gustatory response
CC (PubMed:18687026). However, it was later shown that it acts as a
CC photoreceptor for short wavelength (UV) light (PubMed:27863243).
CC {ECO:0000269|PubMed:18687026, ECO:0000269|PubMed:27863243}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=A taste of light - Issue 194
CC of August 2017;
CC URL="https://web.expasy.org/spotlight/back_issues/194/";
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DR EMBL; BX284606; CCD64541.1; -; Genomic_DNA.
DR EMBL; AF387605; AAK70488.1; -; mRNA.
DR PIR; T15490; T15490.
DR RefSeq; NP_509043.3; NM_076642.5.
DR AlphaFoldDB; Q17990; -.
DR SMR; Q17990; -.
DR STRING; 6239.C14F11.3; -.
DR TCDB; 1.A.69.3.5; the heteromeric odorant receptor channel (horc) family.
DR PaxDb; Q17990; -.
DR EnsemblMetazoa; C14F11.3.1; C14F11.3.1; WBGene00001803.
DR GeneID; 180894; -.
DR KEGG; cel:CELE_C14F11.3; -.
DR UCSC; C14F11.3; c. elegans.
DR CTD; 180894; -.
DR WormBase; C14F11.3; CE29579; WBGene00001803; lite-1.
DR eggNOG; ENOG502TFJI; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_625900_0_0_1; -.
DR InParanoid; Q17990; -.
DR OMA; TFHEERD; -.
DR OrthoDB; 776225at2759; -.
DR PRO; PR:Q17990; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001803; Expressed in larva and 2 other tissues.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043005; C:neuron projection; IDA:WormBase.
DR GO; GO:0043025; C:neuronal cell body; IDA:WormBase.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0009882; F:blue light photoreceptor activity; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISS:WormBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; NAS:UniProtKB.
DR GO; GO:0007626; P:locomotory behavior; IMP:WormBase.
DR GO; GO:0046957; P:negative phototaxis; IMP:UniProtKB.
DR GO; GO:0007602; P:phototransduction; IMP:UniProtKB.
DR GO; GO:0009637; P:response to blue light; IMP:WormBase.
DR GO; GO:0009411; P:response to UV; IMP:WormBase.
DR GO; GO:0070141; P:response to UV-A; IDA:UniProtKB.
DR GO; GO:0050909; P:sensory perception of taste; IEA:InterPro.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Chromophore; G-protein coupled receptor; Membrane;
KW Photoreceptor protein; Receptor; Reference proteome; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..439
FT /note="High-energy light unresponsive protein 1"
FT /id="PRO_0000352777"
FT TOPO_DOM 1..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 68..88
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..114
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 115..135
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 165..185
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..211
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 212..232
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 291..311
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..324
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 325..345
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 346..402
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27863243"
FT TRANSMEM 403..423
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..439
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:27863243"
FT MUTAGEN 77
FT /note="W->A: Nearly abolished photoreceptor activity."
FT /evidence="ECO:0000269|PubMed:27863243"
FT MUTAGEN 226
FT /note="S->F: In xu10; loss of phototaxis behavior due to
FT impaired photoreceptor activity."
FT /evidence="ECO:0000269|PubMed:20436480,
FT ECO:0000269|PubMed:27863243"
FT MUTAGEN 328
FT /note="W->A: Nearly abolished photoreceptor activity."
FT /evidence="ECO:0000269|PubMed:27863243"
FT MUTAGEN 332
FT /note="A->V: In xu8; loss of phototaxis behavior due to
FT impaired photoreceptor activity."
FT /evidence="ECO:0000269|PubMed:20436480,
FT ECO:0000269|PubMed:27863243"
FT MUTAGEN 401
FT /note="R->H: In xu7; loss of phototaxis behavior. Loss of
FT phototransduction in ASJ neurons. Slightly reduces lifespan
FT under constant white light exposure as compared to wild-
FT type."
FT /evidence="ECO:0000269|PubMed:20436480,
FT ECO:0000269|PubMed:29500338"
SQ SEQUENCE 439 AA; 50554 MW; 92D68C2A67572914 CRC64;
MPPPSSHSNI FHSTFKHTVK ETMANAKKTM IAKILSSRNK WAICDRTLYP IYYLLLILGL
NQSIRPNNSL LFRIYSWLVF CLLLFTTLRK FNQVGVRPNG TRENLQEFFA NPRSMITLCN
ALIMLSGLLA SLQLYTLGAK RLKPLKILCQ FSLNVRTKQA ERRQFMINTF LAVFSGLLAL
TMAATYAMSK WGYILYIVGT PNLDTETIFC VLLDSYALFV SRAAISALAI LFYQHCSVIR
RSIKHLINEM VPAEQDECPL PESSLQKIHD CQISYQRIFN GKAVIEEYYS FVLFYSYGVC
IPIFCFLMFV GMSAQSICWS EVVSIVIWIV NAILVLLLFS LPAFMINEDG DRLVASSFRM
YHETFHEERD LTVLSQMTFF TFQIHSTKLT LSACNYFYMD RSILLSLFSA ILTYFLILWE
FDIKNNQSLQ NIANHTIHT
