LIV4_CRYNH
ID LIV4_CRYNH Reviewed; 625 AA.
AC J9VW97;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 2.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=DNA-binding protein LIV4 {ECO:0000305};
GN Name=LIV4 {ECO:0000303|PubMed:32391887};
GN ORFNames=CNAG_06283 {ECO:0000312|EMBL:AFR98518.2};
OS Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN [1] {ECO:0000312|Proteomes:UP000010091}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA Cuomo C.A., Dietrich F.S.;
RT "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT grubii reveals complex RNA expression and microevolution leading to
RT virulence attenuation.";
RL PLoS Genet. 10:E1004261-E1004261(2014).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=32391887; DOI=10.1093/femsyr/foaa015;
RA Yi J., Sang J., Zhao J., Gao L., Yang Y., Yan L., Zhang C., Pan W.,
RA Wang G., Liao W.;
RT "Transcription factor Liv4 is required for growth and pathogenesis of
RT Cryptococcus neoformans.";
RL FEMS Yeast Res. 20:0-0(2020).
CC -!- FUNCTION: DNA-binding protein that activates transcription of RNA
CC binding protein VTS1/RBP1 (PubMed:32391887). Required for cell
CC integrity (PubMed:32391887). {ECO:0000269|PubMed:32391887}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:32391887}.
CC -!- DISRUPTION PHENOTYPE: Decreases RNA level of genes involved in cell
CC wall biogenesis, fatty acid degradation, and carbon utilization
CC (PubMed:32391887). Ruptured membranes, degranulated rough endoplasmic
CC reticulum, irregular and thin cell wall (PubMed:32391887). Abnormal
CC mitochondrial morphology (PubMed:32391887). Decreases cell population
CC viability (PubMed:32391887). Decreases virulence in a mouse inhalation
CC infection model (PubMed:32391887). {ECO:0000269|PubMed:32391887}.
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DR EMBL; CP003832; AFR98518.2; -; Genomic_DNA.
DR RefSeq; XP_012053274.1; XM_012197884.1.
DR AlphaFoldDB; J9VW97; -.
DR SMR; J9VW97; -.
DR EnsemblFungi; AFR98518; AFR98518; CNAG_06283.
DR GeneID; 23889498; -.
DR VEuPathDB; FungiDB:CNAG_06283; -.
DR HOGENOM; CLU_437430_0_0_1; -.
DR Proteomes; UP000010091; Chromosome 13.
DR GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IC:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR SMART; SM00717; SANT; 3.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
DR PROSITE; PS50090; MYB_LIKE; 1.
PE 4: Predicted;
KW DNA-binding; Nucleus; Transcription; Transcription regulation.
FT CHAIN 1..625
FT /note="DNA-binding protein LIV4"
FT /id="PRO_0000451820"
FT DOMAIN 383..436
FT /note="HTH myb-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 435..513
FT /note="Myb-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT DNA_BIND 410..432
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 603..625
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..100
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 625 AA; 70910 MW; DBDA1EFD47591D2F CRC64;
MEGIELGIQD LDRSYQIDTQ AASSGDEEDR QEVPERPTKR KRTKEEKERR RSEKRARKEK
RRSETAQAAQ IQAPQAQVGI EAEVAEAPAD YDEVEQVVED APVSEDNKKR KEKKHKKDKN
KGKKGGSEEQ EEEDREAIAA SAVATLAQAL VSSESDQVAP PVDQIQTPAR PTTATSTSAT
WRTNPNQYGS TKVKKVPLEL SPPPAASSLT PAPPATQLIP VTPLTAASAG ASSFAVRDKI
NSLKHPKSST NAPKAIRSTR KGEDTKESDA QLRLRFKDPK AQEEWLASTS IGKTELLRLE
KEGILSYKKG KFTEDEKVSI KKALENYQKI HRMSSFDLVE LVMTKTLQAT DKETVREFWK
DIAASVPGRP ILNVQPFVRR MLDPKAHKGR WTSEEDELLL RAYAQHPREW TKISSIVDRT
EVDCRDRYLK ELVNRDTRTA GRWTKEEEDK LEEVVDRVAK GLRAEQVHGE ERETPEEGTE
LVEPSDVPWD IVSKEMGNTR SMTQCRIKYR DAIWPRKLGL GKDDHVGRTL KVLTRLKNLN
YESEKHISWS QVRETLEKYS LKEIRNSYSN LKKSVTSDPH VASLTYPELI KVMYDKAVMQ
RGRKVRADQR DYPSKETVES GDEAY
