ID   LIVB3_RHIL3             Reviewed;         367 AA.
AC   Q1MDE9;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Leu/Ile/Val-binding protein BraC3 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=braC3 {ECO:0000303|PubMed:19597156, ECO:0000312|EMBL:CAK09028.1};
GN   OrderedLocusNames=RL3540 {ECO:0000303|PubMed:19597156,
GN   ECO:0000312|EMBL:CAK09028.1};
OS   Rhizobium leguminosarum bv. viciae (strain 3841).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=216596 {ECO:0000312|EMBL:CAK09028.1};
RN   [1] {ECO:0000312|EMBL:CAK09028.1, ECO:0000312|Proteomes:UP000006575}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3841 {ECO:0000312|EMBL:CAK09028.1,
RC   ECO:0000312|Proteomes:UP000006575};
RX   PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA   Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F.,
RA   Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H.,
RA   East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I.,
RA   Chillingworth T., Clarke K., Cronin A., Davis P., Fraser A., Hance Z.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J.;
RT   "The genome of Rhizobium leguminosarum has recognizable core and accessory
RT   components.";
RL   Genome Biol. 7:R34.1-R34.20(2006).
RN   [2]
RP   FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=3841 {ECO:0000303|PubMed:19597156};
RX   PubMed=19597156; DOI=10.1073/pnas.0903653106;
RA   Prell J., White J.P., Bourdes A., Bunnewell S., Bongaerts R.J., Poole P.S.;
RT   "Legumes regulate Rhizobium bacteroid development and persistence by the
RT   supply of branched-chain amino acids.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:12477-12482(2009).
CC   -!- FUNCTION: Part of the ABC transporter complex BraDEFGC/C3 involved in
CC       transport of branched-chain amino acids Leu, Ile and Val (LIV).
CC       Essential for the development of bacteroids, the differentiated legume-
CC       symbiotic forms of this bacterium, and for the effective N(2) fixation
CC       by them. {ECO:0000269|PubMed:19597156}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (BraF and
CC       BraG), two transmembrane proteins (BraD and BraE) and a solute-binding
CC       protein (BraC or BraC3). {ECO:0000305|PubMed:19597156}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Reduced uptake of amino acids Glu, Asp, Ala, Leu,
CC       Ile and Val by bacteroids that lack this gene and the genes aapJ and
CC       braC from the two ABC uptake systems of branched-chain amino acids. Pea
CC       plants inoculated with the triple mutant bacteroids are defective in
CC       symbiosis; the plants are yellow, have increased numbers of small pale
CC       pink root nodules, dry weights similar to uninoculated control plants
CC       and 30% acetylene reduction compared to plants inoculated with wild-
CC       type bacteroids. {ECO:0000269|PubMed:19597156}.
CC   -!- SIMILARITY: Belongs to the leucine-binding protein family.
CC       {ECO:0000305}.
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DR   EMBL; AM236080; CAK09028.1; -; Genomic_DNA.
DR   RefSeq; WP_011653012.1; NC_008380.1.
DR   AlphaFoldDB; Q1MDE9; -.
DR   SMR; Q1MDE9; -.
DR   STRING; 216596.RL3540; -.
DR   EnsemblBacteria; CAK09028; CAK09028; RL3540.
DR   KEGG; rle:RL3540; -.
DR   eggNOG; COG0683; Bacteria.
DR   HOGENOM; CLU_027128_6_0_5; -.
DR   OMA; MGPYCSG; -.
DR   OrthoDB; 776281at2; -.
DR   Proteomes; UP000006575; Chromosome.
DR   GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IMP:UniProtKB.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0043090; P:amino acid import; IMP:UniProtKB.
DR   GO; GO:0015803; P:branched-chain amino acid transport; IMP:UniProtKB.
DR   GO; GO:0015818; P:isoleucine transport; IMP:UniProtKB.
DR   GO; GO:0015820; P:leucine transport; IMP:UniProtKB.
DR   GO; GO:0015829; P:valine transport; IMP:UniProtKB.
DR   InterPro; IPR028081; Leu-bd.
DR   InterPro; IPR000709; Leu_Ile_Val-bd.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF13458; Peripla_BP_6; 1.
DR   PRINTS; PR00337; LEUILEVALBP.
DR   SUPFAM; SSF53822; SSF53822; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Periplasm; Signal; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..367
FT                   /note="Leu/Ile/Val-binding protein BraC3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5004194190"
SQ   SEQUENCE   367 AA;  37574 MW;  C82ED3CD57E405C1 CRC64;
     MTLKTLTATL VASLAFAPLA HADITIGLIA PLTGPVAAYG DQVKNGAQTA VDEINKKGGI
     LGEKVVLELA DDAGEPKQGV SAANKVVGDG IRFVVGPVTS GVAIPVSDVL AENGVLMVTP
     TATAPDLTKR GLTNVLRTCG RDDQQAEVAA KYVLKNFKDK RVAIVNDKGA YGKGLADAFK
     ATLNAGGITE VVNDAITPGD KDFSALTTRI KSEKVDVVYF GGYHPEGGLL ARQLHDLAAN
     ATIIGGDGLS NTEFWAIGTD AAGGTIFTNA SDATKSPDSK AAADALAAKN IPAEAFTLNA
     YAAVEVLKAG IEKAGSAEDA EAVATALKDG KEIPTAIGKV TYGETGDLTS QSFSLYKWEA
     GKIVAAE