LIVF_ECOLI
ID LIVF_ECOLI Reviewed; 237 AA.
AC P22731; P76697; Q2M7B7;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=High-affinity branched-chain amino acid transport ATP-binding protein LivF;
DE AltName: Full=LIV-I protein F;
GN Name=livF; OrderedLocusNames=b3454, JW3419;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2195019; DOI=10.1016/s0021-9258(19)38417-0;
RA Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K.,
RA Gibson A.L., Oxender D.L.;
RT "Nucleotide sequence and genetic characterization reveal six essential
RT genes for the LIV-I and LS transport systems of Escherichia coli.";
RL J. Biol. Chem. 265:11436-11443(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- FUNCTION: Component of the leucine-specific transport system.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB18429.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; J05516; AAA83887.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18429.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76479.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77839.1; -; Genomic_DNA.
DR PIR; S47673; S47673.
DR RefSeq; NP_417911.4; NC_000913.3.
DR RefSeq; WP_000416891.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P22731; -.
DR SMR; P22731; -.
DR BioGRID; 4261110; 17.
DR BioGRID; 852270; 2.
DR ComplexPortal; CPX-4316; Branched chain amino acid ABC transporter complex.
DR ComplexPortal; CPX-4317; Branched chain amino acid, leucine-specific ABC transporter complex.
DR IntAct; P22731; 13.
DR STRING; 511145.b3454; -.
DR TCDB; 3.A.1.4.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; P22731; -.
DR PRIDE; P22731; -.
DR EnsemblBacteria; AAC76479; AAC76479; b3454.
DR EnsemblBacteria; BAE77839; BAE77839; BAE77839.
DR GeneID; 60670292; -.
DR GeneID; 947961; -.
DR KEGG; ecj:JW3419; -.
DR KEGG; eco:b3454; -.
DR PATRIC; fig|511145.12.peg.3552; -.
DR EchoBASE; EB0531; -.
DR eggNOG; COG0410; Bacteria.
DR HOGENOM; CLU_000604_1_2_6; -.
DR InParanoid; P22731; -.
DR OMA; IMMQKIM; -.
DR PhylomeDB; P22731; -.
DR BioCyc; EcoCyc:LIVF-MON; -.
DR BioCyc; MetaCyc:LIVF-MON; -.
DR PRO; PR:P22731; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR GO; GO:0015658; F:branched-chain amino acid transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015803; P:branched-chain amino acid transport; IDA:EcoCyc.
DR GO; GO:1903714; P:isoleucine transmembrane transport; IDA:EcoCyc.
DR GO; GO:0015807; P:L-amino acid transport; IBA:GO_Central.
DR GO; GO:1903785; P:L-valine transmembrane transport; IDA:EcoCyc.
DR GO; GO:0015823; P:phenylalanine transport; IDA:EcoCyc.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR030660; ABC_branched_ATPase_LivF/BraG.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR PIRSF; PIRSF039137; ABC_branched_ATPase; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; ATP-binding; Nucleotide-binding; Reference proteome;
KW Transport.
FT CHAIN 1..237
FT /note="High-affinity branched-chain amino acid transport
FT ATP-binding protein LivF"
FT /id="PRO_0000092401"
FT DOMAIN 6..237
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CONFLICT 56
FT /note="R -> G (in Ref. 1; AAA83887)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="V -> A (in Ref. 1; AAA83887)"
FT /evidence="ECO:0000305"
FT CONFLICT 200
FT /note="A -> G (in Ref. 1; AAA83887)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 237 AA; 26310 MW; 7E4129E54ED1BD02 CRC64;
MEKVMLSFDK VSAHYGKIQA LHEVSLHINQ GEIVTLIGAN GAGKTTLLGT LCGDPRATSG
RIVFDDKDIT DWQTAKIMRE AVAIVPEGRR VFSRMTVEEN LAMGGFFAER DQFQERIKWV
YELFPRLHER RIQRAGTMSG GEQQMLAIGR ALMSNPRLLL LDEPSLGLAP IIIQQIFDTI
EQLREQGMTI FLVEQNANQA LKLADRGYVL ENGHVVLSDT GDALLANEAV RSAYLGG
