LIVM_ECOLI
ID LIVM_ECOLI Reviewed; 425 AA.
AC P22729; Q2M7B9;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=High-affinity branched-chain amino acid transport system permease protein LivM;
DE AltName: Full=LIV-I protein M;
GN Name=livM; OrderedLocusNames=b3456, JW3421;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2195019; DOI=10.1016/s0021-9258(19)38417-0;
RA Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K.,
RA Gibson A.L., Oxender D.L.;
RT "Nucleotide sequence and genetic characterization reveal six essential
RT genes for the LIV-I and LS transport systems of Escherichia coli.";
RL J. Biol. Chem. 265:11436-11443(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Part of the binding-protein-dependent transport system for
CC branched-chain amino acids. Probably responsible for the translocation
CC of the substrates across the membrane.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. LivHM subfamily. {ECO:0000305}.
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DR EMBL; J05516; AAA83885.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18431.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76481.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77837.1; -; Genomic_DNA.
DR PIR; S47675; S47675.
DR RefSeq; NP_417913.1; NC_000913.3.
DR RefSeq; WP_000803813.1; NZ_LN832404.1.
DR AlphaFoldDB; P22729; -.
DR BioGRID; 4262489; 17.
DR ComplexPortal; CPX-4316; Branched chain amino acid ABC transporter complex.
DR ComplexPortal; CPX-4317; Branched chain amino acid, leucine-specific ABC transporter complex.
DR STRING; 511145.b3456; -.
DR TCDB; 3.A.1.4.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; P22729; -.
DR PRIDE; P22729; -.
DR EnsemblBacteria; AAC76481; AAC76481; b3456.
DR EnsemblBacteria; BAE77837; BAE77837; BAE77837.
DR GeneID; 947966; -.
DR KEGG; ecj:JW3421; -.
DR KEGG; eco:b3456; -.
DR PATRIC; fig|1411691.4.peg.3270; -.
DR EchoBASE; EB0536; -.
DR eggNOG; COG4177; Bacteria.
DR HOGENOM; CLU_031365_1_1_6; -.
DR InParanoid; P22729; -.
DR OMA; VFWYKLQ; -.
DR PhylomeDB; P22729; -.
DR BioCyc; EcoCyc:LIVM-MON; -.
DR BioCyc; MetaCyc:LIVM-MON; -.
DR PRO; PR:P22729; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015658; F:branched-chain amino acid transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015192; F:L-phenylalanine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015803; P:branched-chain amino acid transport; IDA:EcoCyc.
DR GO; GO:1903714; P:isoleucine transmembrane transport; IDA:EcoCyc.
DR GO; GO:1903785; P:L-valine transmembrane transport; IDA:EcoCyc.
DR GO; GO:0015823; P:phenylalanine transport; IDA:EcoCyc.
DR CDD; cd06581; TM_PBP1_LivM_like; 1.
DR InterPro; IPR001851; ABC_transp_permease.
DR InterPro; IPR021807; LivHM_N.
DR InterPro; IPR043428; LivM-like.
DR PANTHER; PTHR30482; PTHR30482; 1.
DR Pfam; PF02653; BPD_transp_2; 1.
DR Pfam; PF11862; DUF3382; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..425
FT /note="High-affinity branched-chain amino acid transport
FT system permease protein LivM"
FT /id="PRO_0000060062"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 150..178
FT /note="AYTFALLNHYYGLGFWTCLPIAGLMAAAA -> LHFCAAQSLLRLGLLDLPA
FT DCWINGSGG (in Ref. 1; AAA83885)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="R -> G (in Ref. 1; AAA83885)"
FT /evidence="ECO:0000305"
FT CONFLICT 411
FT /note="P -> R (in Ref. 1; AAA83885)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 425 AA; 46269 MW; F9658D0E5FCE987F CRC64;
MKPMHIAMAL LSAAMFFVLA GVFMGVQLEL DGTKLVVDTA SDVRWQWVFI GTAVVFFFQL
LRPAFQKGLK SVSGPKFILP AIDGSTVKQK LFLVALLVLA VAWPFMVSRG TVDIATLTMI
YIILGLGLNV VVGLSGLLVL GYGGFYAIGA YTFALLNHYY GLGFWTCLPI AGLMAAAAGF
LLGFPVLRLR GDYLAIVTLG FGEIVRILLL NNTEITGGPN GISQIPKPTL FGLEFSRTAR
EGGWDTFSNF FGLKYDPSDR VIFLYLVALL LVVLSLFVIN RLLRMPLGRA WEALREDEIA
CRSLGLSPRR IKLTAFTISA AFAGFAGTLF AARQGFVSPE SFTFAESAFV LAIVVLGGMG
SQFAVILAAI LLVVSRELMR DFNEYSMLML GGLMVLMMIW RPQGLLPMTR PQLKLKNGAA
KGEQA
