LIX1_DROME
ID LIX1_DROME Reviewed; 268 AA.
AC Q9VKY1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Protein limb expression 1 homolog {ECO:0000303|PubMed:19710173};
DE AltName: Full=lowfat {ECO:0000303|PubMed:19710173};
GN Name=lft {ECO:0000312|FlyBase:FBgn0032230};
GN ORFNames=CG13139 {ECO:0000312|FlyBase:FBgn0032230};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ABC86412.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ABC86412.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH DS AND FT, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19710173; DOI=10.1242/dev.036152;
RA Mao Y., Kucuk B., Irvine K.D.;
RT "Drosophila lowfat, a novel modulator of Fat signaling.";
RL Development 136:3223-3233(2009).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=21379328; DOI=10.1371/journal.pgen.1001305;
RA Hogan J., Valentine M., Cox C., Doyle K., Collier S.;
RT "Two frizzled planar cell polarity signals in the Drosophila wing are
RT differentially organized by the Fat/Dachsous pathway.";
RL PLoS Genet. 7:E1001305-E1001305(2011).
CC -!- FUNCTION: Component of the Fat (ft) signaling pathway that functions in
CC normal development of various organs such as the wing and leg
CC (PubMed:19710173, PubMed:21379328). In developing imaginal disks,
CC involved in regulating both the protein levels and apical localization
CC of ft and ds (PubMed:19710173). Involved in establishing planar cell
CC polarity (PCP) along the anterior-posterior axis of the wing (the early
CC Fz signaling event), probably by acting upstream of ds and ft to
CC regulate Fz activity (PubMed:21379328). {ECO:0000269|PubMed:19710173,
CC ECO:0000269|PubMed:21379328}.
CC -!- SUBUNIT: Interacts with ft (via intracellular domain) and ds (via
CC intracellular domain). {ECO:0000269|PubMed:19710173}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000269|PubMed:19710173}; Peripheral membrane protein
CC {ECO:0000269|PubMed:19710173}. Cytoplasm {ECO:0000269|PubMed:19710173}.
CC Note=In wing imaginal disks, strong cytoplasmic expression in the wing
CC pouch but in regions of the wing hinge preferentially expressed in the
CC sub-apical membrane. {ECO:0000269|PubMed:19710173}.
CC -!- DEVELOPMENTAL STAGE: Broadly expressed in the developing imaginal disks
CC including the wing, leg and eye, and the neuroepithelium of the optic
CC lobes of the brain. In the eye imaginal disk, expression is highest in
CC the morphogenetic furrow and in the wing imaginal disk it is highest
CC near the dorsal-ventral (DV) compartment boundary.
CC {ECO:0000269|PubMed:19710173}.
CC -!- DISRUPTION PHENOTYPE: Viable and fertile. Wings are slightly shorter
CC with a decreased distance between cross-veins.
CC {ECO:0000269|PubMed:19710173}.
CC -!- SIMILARITY: Belongs to the LIX1 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52926.1; -; Genomic_DNA.
DR EMBL; AE014134; ADV37025.1; -; Genomic_DNA.
DR EMBL; BT024350; ABC86412.1; -; mRNA.
DR RefSeq; NP_001188775.1; NM_001201846.2.
DR RefSeq; NP_609390.1; NM_135546.3.
DR AlphaFoldDB; Q9VKY1; -.
DR IntAct; Q9VKY1; 4.
DR STRING; 7227.FBpp0079592; -.
DR PaxDb; Q9VKY1; -.
DR PRIDE; Q9VKY1; -.
DR DNASU; 34405; -.
DR EnsemblMetazoa; FBtr0080002; FBpp0079592; FBgn0032230.
DR EnsemblMetazoa; FBtr0302490; FBpp0291652; FBgn0032230.
DR GeneID; 34405; -.
DR KEGG; dme:Dmel_CG13139; -.
DR UCSC; CG13139-RA; d. melanogaster.
DR CTD; 34405; -.
DR FlyBase; FBgn0032230; lft.
DR VEuPathDB; VectorBase:FBgn0032230; -.
DR eggNOG; ENOG502QR91; Eukaryota.
DR GeneTree; ENSGT00390000005869; -.
DR HOGENOM; CLU_065651_0_1_1; -.
DR InParanoid; Q9VKY1; -.
DR OMA; VAREHES; -.
DR OrthoDB; 1026341at2759; -.
DR PhylomeDB; Q9VKY1; -.
DR Reactome; R-DME-390150; DS ligand bound to FT receptor.
DR BioGRID-ORCS; 34405; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34405; -.
DR PRO; PR:Q9VKY1; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032230; Expressed in wing disc and 19 other tissues.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0035003; C:subapical complex; IDA:FlyBase.
DR GO; GO:0097352; P:autophagosome maturation; IBA:GO_Central.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR InterPro; IPR029270; LIX1.
DR Pfam; PF14954; LIX1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Membrane; Reference proteome.
FT CHAIN 1..268
FT /note="Protein limb expression 1 homolog"
FT /id="PRO_0000438291"
SQ SEQUENCE 268 AA; 30698 MW; 5125B94DB5169C74 CRC64;
MVYPEEPFWV LPTVTGFYED RDYRVNVVEA LQEFWQMKQS RGAELKNGAL VIYESIPSNS
QPYICFVTLP GGSCFGSFQN CPTKAEARRS SAKIALMNSV FNEHPSRRIS DEFIQKAVQD
ARTSFKGTSQ INEGTESGIG AFRFMLEANK GRTMLEFQEL MTVFQLLHWN GSLKAMRERH
CSRQEVVAHY SNRSLDDEMR SQMALDWIAR EHDNPGVIRR ELVLAERELE TFRMAGRELR
FPKEKKDILM IAHNQLGGSA LNSATIDD
