LKA1A_MANHA
ID LKA1A_MANHA Reviewed; 953 AA.
AC P16535; Q9ETG5;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Leukotoxin;
DE Short=Lkt;
GN Name=lktA;
OS Mannheimia haemolytica (Pasteurella haemolytica).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Mannheimia.
OX NCBI_TaxID=75985;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype A1;
RX PubMed=3040588; DOI=10.1128/iai.55.9.1987-1996.1987;
RA Lo R.Y.C., Strathdee C.A., Shewen P.E.;
RT "Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica
RT A1.";
RL Infect. Immun. 55:1987-1996(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype A1 / PH101;
RX PubMed=2707120; DOI=10.1089/dna.1.1989.8.15;
RA Highlander S.K., Chidambaram M., Engler M.J., Weinstock G.M.;
RT "DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster.";
RL DNA 8:15-28(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype A1 / PH8;
RX PubMed=11157953; DOI=10.1128/jb.183.4.1394-1404.2001;
RA Davies R.L., Whittam T.S., Selander R.K.;
RT "Sequence diversity and molecular evolution of the leukotoxin (lktA) gene
RT in bovine and ovine strains of Mannheimia (Pasteurella) haemolytica.";
RL J. Bacteriol. 183:1394-1404(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 884-953.
RC STRAIN=Serotype A1 / PH101;
RX PubMed=2185213; DOI=10.1128/jb.172.5.2343-2350.1990;
RA Highlander S.K., Engler M.J., Weinstock G.M.;
RT "Secretion and expression of the Pasteurella haemolytica Leukotoxin.";
RL J. Bacteriol. 172:2343-2350(1990).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 947-953.
RC STRAIN=Serotype A1;
RX PubMed=2914876; DOI=10.1128/jb.171.2.916-928.1989;
RA Strathdee C.A., Lo R.Y.C.;
RT "Cloning, nucleotide sequence, and characterization of genes encoding the
RT secretion function of the Pasteurella haemolytica leukotoxin determinant.";
RL J. Bacteriol. 171:916-928(1989).
RN [6]
RP IDENTIFICATION OF THE EXPORT SIGNAL.
RX PubMed=8483936; DOI=10.1073/pnas.90.9.4211;
RA Zhang F., Greig D.I., Ling V.;
RT "Functional replacement of the hemolysin A transport signal by a different
RT primary sequence.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:4211-4215(1993).
RN [7]
RP REVIEW.
RX PubMed=11750142; DOI=10.1016/s0378-1135(01)00467-9;
RA Narayanan S.K., Nagaraja T.G., Chengappa M.M., Stewart G.C.;
RT "Leukotoxins of Gram-negative bacteria.";
RL Vet. Microbiol. 84:337-356(2002).
CC -!- FUNCTION: Pasteurella leukotoxins are exotoxins that attack host
CC leukocytes and especially polymorphonuclear cells, by causing cell
CC rupture. The leukotoxin binds to the host LFA-1 integrin and induces a
CC signaling cascade leading to many biological effects, including
CC tyrosine phosphorylation of the CD18 tail, elevation of the
CC intracellular Ca(2+) and lysis of the host cell. This leukotoxin is a
CC major contributor to the pathogenesis of lung injury in ovine pneumonic
CC pasteurellosis. It has also week hemolytic activity.
CC -!- SUBCELLULAR LOCATION: Secreted. Host cell membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- DOMAIN: The transmembrane domains are believed to be involved in pore
CC formation in target cells.
CC -!- DOMAIN: The Gly-rich region is probably involved in calcium binding,
CC which is required for target cell-binding and cytolytic activity.
CC -!- DOMAIN: The C-terminal domain contains an export signal that is
CC recognized by the ABC transporter complex LktBD.
CC -!- PTM: Acylated by LktC. The toxin only becomes active when modified.
CC -!- MISCELLANEOUS: The lktCABD operon has a complex mosaic structure that
CC has been derived by extensive inter- and intraspecies horizontal DNA
CC transfer and intragenic recombination events.
CC -!- SIMILARITY: Belongs to the RTX prokaryotic toxin (TC 1.C.11) family.
CC {ECO:0000305}.
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DR EMBL; M20730; AAA25529.1; -; Genomic_DNA.
DR EMBL; M24197; AAA25543.1; -; Genomic_DNA.
DR EMBL; AF314506; AAG40290.1; -; Genomic_DNA.
DR PIR; B30169; B30169.
DR RefSeq; WP_006248023.1; NZ_VAJK01000035.1.
DR RefSeq; WP_015484526.1; NZ_JAFIRP010000016.1.
DR AlphaFoldDB; P16535; -.
DR SMR; P16535; -.
DR STRING; 75985.WC39_13370; -.
DR GeneID; 67370328; -.
DR GO; GO:0005615; C:extracellular space; IMP:CAFA.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR GO; GO:1901998; P:toxin transport; IMP:CAFA.
DR DisProt; DP00345; -.
DR Gene3D; 2.150.10.10; -; 1.
DR InterPro; IPR018511; Hemolysin-typ_Ca-bd_CS.
DR InterPro; IPR001343; Hemolysn_Ca-bd.
DR InterPro; IPR013550; RTX_C.
DR InterPro; IPR018504; RTX_N.
DR InterPro; IPR003995; RTX_toxin_determinant-A.
DR InterPro; IPR011049; Serralysin-like_metalloprot_C.
DR Pfam; PF00353; HemolysinCabind; 3.
DR Pfam; PF02382; RTX; 1.
DR Pfam; PF08339; RTX_C; 1.
DR PRINTS; PR01488; RTXTOXINA.
DR SUPFAM; SSF51120; SSF51120; 1.
DR PROSITE; PS00330; HEMOLYSIN_CALCIUM; 4.
PE 3: Inferred from homology;
KW Calcium; Cytolysis; Hemolysis; Host cell membrane; Host membrane;
KW Lipoprotein; Membrane; Repeat; Secreted; Toxin; Transmembrane;
KW Transmembrane helix; Virulence.
FT CHAIN 1..953
FT /note="Leukotoxin"
FT /id="PRO_0000196219"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 715..732
FT /note="Hemolysin-type calcium-binding 1"
FT REPEAT 733..750
FT /note="Hemolysin-type calcium-binding 2"
FT REPEAT 751..768
FT /note="Hemolysin-type calcium-binding 3"
FT REPEAT 769..786
FT /note="Hemolysin-type calcium-binding 4"
FT REPEAT 789..806
FT /note="Hemolysin-type calcium-binding 5"
FT CONFLICT 409..414
FT /note="FEHVAN -> LSTLQI (in Ref. 2; AAA25543)"
FT /evidence="ECO:0000305"
FT CONFLICT 742
FT /note="D -> Y (in Ref. 2; AAA25543)"
FT /evidence="ECO:0000305"
FT CONFLICT 839
FT /note="N -> D (in Ref. 3; AAG40290)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 953 AA; 101997 MW; 7F93D113A118C05F CRC64;
MGTRLTTLSN GLKNTLTATK SGLHKAGQSL TQAGSSLKTG AKKIILYIPQ NYQYDTEQGN
GLQDLVKAAE ELGIEVQREE RNNIATAQTS LGTIQTAIGL TERGIVLSAP QIDKLLQKTK
AGQALGSAES IVQNANKAKT VLSGIQSILG SVLAGMDLDE ALQNNSNQHA LAKAGLELTN
SLIENIANSV KTLDEFGEQI SQFGSKLQNI KGLGTLGDKL KNIGGLDKAG LGLDVISGLL
SGATAALVLA DKNASTAKKV GAGFELANQV VGNITKAVSS YILAQRVAAG LSSTGPVAAL
IASTVSLAIS PLAFAGIADK FNHAKSLESY AERFKKLGYD GDNLLAEYQR GTGTIDASVT
AINTALAAIA GGVSAAAAGS VIASPIALLV SGITGVISTI LQYSKQAMFE HVANKIHNKI
VEWEKNNHGK NYFENGYDAR YLANLQDNMK FLLNLNKELQ AERVIAITQQ QWDNNIGDLA
GISRLGEKVL SGKAYVDAFE EGKHIKADKL VQLDSANGII DVSNSGKAKT QHILFRTPLL
TPGTEHRERV QTGKYEYITK LNINRVDSWK ITDGAASSTF DLTNVVQRIG IELDNAGNVT
KTKETKIIAK LGEGDDNVFV GSGTTEIDGG EGYDRVHYSR GNYGALTIDA TKETEQGSYT
VNRFVETGKA LHEVTSTHTA LVGNREEKIE YRHSNNQHHA GYYTKDTLKA VEEIIGTSHN
DIFKGSKFND AFNGGDGVDT IDGNDGNDRL FGGKGDDILD GGNGDDFIDG GKGNDLLHGG
KGDDIFVHRK GDGNDIITDS DGNDKLSFSD SNLKDLTFEK VKHNLVITNS KKEKVTIQNW
FREADFAKEV PNYKATKDEK IEEIIGQNGE RITSKQVDDL IAKGNGKITQ DELSKVVDNY
ELLKHSKNVT NSLDKLISSV SAFTSSNDSR NVLVAPTSML DQSLSSLQFA RAA
