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LLDD_ECOLI
ID   LLDD_ECOLI              Reviewed;         396 AA.
AC   P33232; Q2M7R9;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 166.
DE   RecName: Full=L-lactate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01559};
DE            EC=1.1.-.- {ECO:0000255|HAMAP-Rule:MF_01559};
GN   Name=lldD {ECO:0000255|HAMAP-Rule:MF_01559}; Synonyms=lctD;
GN   OrderedLocusNames=b3605, JW3580;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=K12;
RX   PubMed=8407843; DOI=10.1128/jb.175.20.6671-6678.1993;
RA   Dong J.M., Taylor J.S., Latour D.J., Iuchi S., Lin E.C.C.;
RT   "Three overlapping lct genes involved in L-lactate utilization by
RT   Escherichia coli.";
RL   J. Bacteriol. 175:6671-6678(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA   Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT   "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT   from 76.0 to 81.5 minutes.";
RL   Nucleic Acids Res. 22:2576-2586(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBSTRATE SPECIFICITY,
RP   BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, SUBUNIT, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=18473; DOI=10.1016/s0021-9258(17)40096-2;
RA   Futai M., Kimura H.;
RT   "Inducible membrane-bound L-lactate dehydrogenase from Escherichia coli.
RT   Purification and properties.";
RL   J. Biol. Chem. 252:5820-5827(1977).
CC   -!- FUNCTION: Catalyzes the conversion of L-lactate to pyruvate. Seems to
CC       be a primary dehydrogenase in the respiratory chain. To a lesser
CC       extent, can also oxidize DL-alpha-hydroxybutyrate, but not D-lactate.
CC       {ECO:0000269|PubMed:18473, ECO:0000269|PubMed:8407843}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-lactate + A = AH2 + pyruvate; Xref=Rhea:RHEA:45816,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15361, ChEBI:CHEBI:16651,
CC         ChEBI:CHEBI:17499; Evidence={ECO:0000255|HAMAP-Rule:MF_01559,
CC         ECO:0000269|PubMed:18473};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01559, ECO:0000269|PubMed:18473};
CC   -!- ACTIVITY REGULATION: Is activated by 2-fold in the presence of high
CC       concentrations of sodium and potassium ions. Is almost completely
CC       inhibited by a high concentration of pyruvate (10 mM).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=120 uM for L-lactate {ECO:0000269|PubMed:18473};
CC         KM=770 uM for DL-alpha-hydroxybutyrate {ECO:0000269|PubMed:18473};
CC       pH dependence:
CC         Optimum pH is 8-9. {ECO:0000269|PubMed:18473};
CC   -!- SUBUNIT: Forms homooligomers. {ECO:0000269|PubMed:18473}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01559, ECO:0000269|PubMed:18473}; Peripheral membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01559, ECO:0000269|PubMed:18473}.
CC   -!- INDUCTION: Is induced by aerobic growth on L-lactate, but not by
CC       aerobic growth on D-lactate or glycerol or by anaerobic growth on
CC       glucose. {ECO:0000269|PubMed:18473, ECO:0000269|PubMed:8407843}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene lose the ability to grow
CC       on L-lactate as the sole source of carbon and energy, but can still
CC       utilize D-lactate. {ECO:0000269|PubMed:8407843}.
CC   -!- SIMILARITY: Belongs to the FMN-dependent alpha-hydroxy acid
CC       dehydrogenase family. {ECO:0000255|HAMAP-Rule:MF_01559}.
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DR   EMBL; L13970; AAA03585.1; -; Unassigned_DNA.
DR   EMBL; U00039; AAB18582.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76629.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77687.1; -; Genomic_DNA.
DR   PIR; C49904; C49904.
DR   RefSeq; NP_418062.1; NC_000913.3.
DR   RefSeq; WP_000586962.1; NZ_SSZK01000022.1.
DR   AlphaFoldDB; P33232; -.
DR   SMR; P33232; -.
DR   BioGRID; 4262561; 51.
DR   DIP; DIP-10108N; -.
DR   IntAct; P33232; 16.
DR   MINT; P33232; -.
DR   STRING; 511145.b3605; -.
DR   jPOST; P33232; -.
DR   PaxDb; P33232; -.
DR   PRIDE; P33232; -.
DR   EnsemblBacteria; AAC76629; AAC76629; b3605.
DR   EnsemblBacteria; BAE77687; BAE77687; BAE77687.
DR   GeneID; 67417608; -.
DR   GeneID; 948121; -.
DR   KEGG; ecj:JW3580; -.
DR   KEGG; eco:b3605; -.
DR   PATRIC; fig|1411691.4.peg.3101; -.
DR   EchoBASE; EB1906; -.
DR   eggNOG; COG1304; Bacteria.
DR   HOGENOM; CLU_020639_0_0_6; -.
DR   InParanoid; P33232; -.
DR   OMA; FTRLMQT; -.
DR   PhylomeDB; P33232; -.
DR   BioCyc; EcoCyc:L-LACTDEHYDROGFMN-MON; -.
DR   BioCyc; MetaCyc:L-LACTDEHYDROGFMN-MON; -.
DR   SABIO-RK; P33232; -.
DR   PRO; PR:P33232; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0010181; F:FMN binding; IDA:EcoCyc.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IDA:EcoCyc.
DR   GO; GO:0009060; P:aerobic respiration; IEP:EcoCyc.
DR   GO; GO:0042355; P:L-fucose catabolic process; IEP:EcoCyc.
DR   GO; GO:0019516; P:lactate oxidation; IMP:EcoCyc.
DR   CDD; cd02809; alpha_hydroxyacid_oxid_FMN; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_01559; L_lact_dehydr; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR012133; Alpha-hydoxy_acid_DH_FMN.
DR   InterPro; IPR000262; FMN-dep_DH.
DR   InterPro; IPR037396; FMN_HAD.
DR   InterPro; IPR008259; FMN_hydac_DH_AS.
DR   InterPro; IPR020920; LldD.
DR   Pfam; PF01070; FMN_dh; 1.
DR   PIRSF; PIRSF000138; Al-hdrx_acd_dh; 1.
DR   PROSITE; PS00557; FMN_HYDROXY_ACID_DH_1; 1.
DR   PROSITE; PS51349; FMN_HYDROXY_ACID_DH_2; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Flavoprotein; FMN; Membrane;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..396
FT                   /note="L-lactate dehydrogenase"
FT                   /id="PRO_0000206335"
FT   DOMAIN          1..380
FT                   /note="FMN hydroxy acid dehydrogenase"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   ACT_SITE        275
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         24
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         106
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         127
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         129
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         155
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         164
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         251
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         278
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
FT   BINDING         306..330
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01559"
SQ   SEQUENCE   396 AA;  42728 MW;  CA0D8E308713BF83 CRC64;
     MIISAASDYR AAAQRILPPF LFHYMDGGAY SEYTLRRNVE DLSEVALRQR ILKNMSDLSL
     ETTLFNEKLS MPVALAPVGL CGMYARRGEV QAAKAADAHG IPFTLSTVSV CPIEEVAPAI
     KRPMWFQLYV LRDRGFMRNA LERAKAAGCS TLVFTVDMPT PGARYRDAHS GMSGPNAAMR
     RYLQAVTHPQ WAWDVGLNGR PHDLGNISAY LGKPTGLEDY IGWLGNNFDP SISWKDLEWI
     RDFWDGPMVI KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGD
     IAILADSGIR NGLDVVRMIA LGADTVLLGR AFLYALATAG QAGVANLLNL IEKEMKVAMT
     LTGAKSISEI TQDSLVQGLG KELPAALAPM AKGNAA
 
 
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