LLG2_ARATH
ID LLG2_ARATH Reviewed; 163 AA.
AC Q6NLF4; Q9SKU7;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=GPI-anchored protein LLG2 {ECO:0000305};
DE AltName: Full=LORELEI-like-GPI-anchored protein 2 {ECO:0000303|PubMed:20163554};
DE Flags: Precursor;
GN Name=LLG2 {ECO:0000303|PubMed:20163554};
GN OrderedLocusNames=At2g20700 {ECO:0000312|Araport:AT2G20700};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20163554; DOI=10.1111/j.1365-313x.2010.04177.x;
RA Tsukamoto T., Qin Y., Huang Y., Dunatunga D., Palanivelu R.;
RT "A role for LORELEI, a putative glycosylphosphatidylinositol-anchored
RT protein, in Arabidopsis thaliana double fertilization and early seed
RT development.";
RL Plant J. 62:571-588(2010).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.77 ANGSTROMS) OF 42-125, AND GLYCOSYLATION AT
RP ASN-52.
RA Xiao Y., Chai J.;
RT "Crystal structure of plant peptide RALF23 in complex with FER and LLG2.";
RL Submitted (JUN-2018) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9FKT1};
CC Lipid-anchor, GPI-anchor {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in pollen, pollen tubes, sporophytic
CC pistil tissues, in the early stages of female gametophyte development,
CC and in unfertilized, mature ovules. {ECO:0000269|PubMed:20163554}.
CC -!- DISRUPTION PHENOTYPE: No aborted seed phenotype and normal production
CC of seed sets. {ECO:0000269|PubMed:20163554}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD20925.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006234; AAD20925.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07058.1; -; Genomic_DNA.
DR EMBL; BT012124; AAS76219.1; -; mRNA.
DR EMBL; BT012380; AAS88770.1; -; mRNA.
DR PIR; C84592; C84592.
DR RefSeq; NP_179662.2; NM_127634.4.
DR PDB; 6A5E; X-ray; 2.77 A; C/D=42-125.
DR PDBsum; 6A5E; -.
DR AlphaFoldDB; Q6NLF4; -.
DR SMR; Q6NLF4; -.
DR STRING; 3702.AT2G20700.1; -.
DR PaxDb; Q6NLF4; -.
DR PRIDE; Q6NLF4; -.
DR ProteomicsDB; 238422; -.
DR EnsemblPlants; AT2G20700.1; AT2G20700.1; AT2G20700.
DR GeneID; 816598; -.
DR Gramene; AT2G20700.1; AT2G20700.1; AT2G20700.
DR KEGG; ath:AT2G20700; -.
DR Araport; AT2G20700; -.
DR TAIR; locus:2051389; AT2G20700.
DR eggNOG; ENOG502S17V; Eukaryota.
DR HOGENOM; CLU_119747_0_0_1; -.
DR InParanoid; Q6NLF4; -.
DR OMA; SDCARTM; -.
DR OrthoDB; 1487552at2759; -.
DR PhylomeDB; Q6NLF4; -.
DR PRO; PR:Q6NLF4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6NLF4; baseline and differential.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:1903427; P:negative regulation of reactive oxygen species biosynthetic process; IGI:TAIR.
DR InterPro; IPR039307; LORELEI-like.
DR PANTHER; PTHR31533; PTHR31533; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein;
KW Membrane; Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..135
FT /note="GPI-anchored protein LLG2"
FT /evidence="ECO:0000255"
FT /id="PRO_5008176503"
FT PROPEP 136..163
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000438101"
FT LIPID 135
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498,
FT ECO:0000269|Ref.5, ECO:0007744|PDB:6A5E"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 54..57
FT /evidence="ECO:0007829|PDB:6A5E"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 67..78
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 82..85
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 92..104
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 110..114
FT /evidence="ECO:0007829|PDB:6A5E"
SQ SEQUENCE 163 AA; 17777 MW; 3B5A657B1A6A5F56 CRC64;
MEISPYCLLS LLPIFLLSGF SLSYDEFDGH AATSRALLQT RTTCKEDFAN KNYTIITSRC
KGPNYPANVC CSAFKDFACP FAEVLNDEKN DCASTMFSYI NLYGRYPPGI FANMCKEGKE
GLDCTDVTQS ASATSDSIPR ASTTASLAVL STFLVLCLLF LSS
