LLG3_ARATH
ID LLG3_ARATH Reviewed; 160 AA.
AC Q9M0I0; Q6NMM2;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=GPI-anchored protein LLG3 {ECO:0000305};
DE AltName: Full=LORELEI-like-GPI-anchored protein 3 {ECO:0000303|PubMed:20163554};
DE Flags: Precursor;
GN Name=LLG3 {ECO:0000303|PubMed:20163554};
GN OrderedLocusNames=At4g28280 {ECO:0000312|Araport:AT4G28280};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20163554; DOI=10.1111/j.1365-313x.2010.04177.x;
RA Tsukamoto T., Qin Y., Huang Y., Dunatunga D., Palanivelu R.;
RT "A role for LORELEI, a putative glycosylphosphatidylinositol-anchored
RT protein, in Arabidopsis thaliana double fertilization and early seed
RT development.";
RL Plant J. 62:571-588(2010).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9FKT1};
CC Lipid-anchor, GPI-anchor {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9M0I0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9M0I0-2; Sequence=VSP_058609;
CC -!- TISSUE SPECIFICITY: Expressed in pollen, pollen tubes, sporophytic
CC pistil tissues, in the early stages of female gametophyte development,
CC and in unfertilized, mature ovules. {ECO:0000269|PubMed:20163554}.
CC -!- DISRUPTION PHENOTYPE: No aborted seed phenotype and normal production
CC of seed sets. {ECO:0000269|PubMed:20163554}.
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DR EMBL; AL161572; CAB79630.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85463.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85464.1; -; Genomic_DNA.
DR EMBL; BT010922; AAR24700.1; -; mRNA.
DR EMBL; BT011635; AAS47641.1; -; mRNA.
DR PIR; T09044; T09044.
DR RefSeq; NP_001190858.1; NM_001203929.2. [Q9M0I0-1]
DR RefSeq; NP_194557.2; NM_118968.2. [Q9M0I0-2]
DR AlphaFoldDB; Q9M0I0; -.
DR SMR; Q9M0I0; -.
DR STRING; 3702.AT4G28280.2; -.
DR PaxDb; Q9M0I0; -.
DR PRIDE; Q9M0I0; -.
DR ProteomicsDB; 238461; -. [Q9M0I0-1]
DR EnsemblPlants; AT4G28280.1; AT4G28280.1; AT4G28280. [Q9M0I0-2]
DR EnsemblPlants; AT4G28280.2; AT4G28280.2; AT4G28280. [Q9M0I0-1]
DR GeneID; 828943; -.
DR Gramene; AT4G28280.1; AT4G28280.1; AT4G28280. [Q9M0I0-2]
DR Gramene; AT4G28280.2; AT4G28280.2; AT4G28280. [Q9M0I0-1]
DR KEGG; ath:AT4G28280; -.
DR Araport; AT4G28280; -.
DR TAIR; locus:2123673; AT4G28280.
DR eggNOG; ENOG502S17V; Eukaryota.
DR HOGENOM; CLU_119747_0_0_1; -.
DR InParanoid; Q9M0I0; -.
DR OMA; AFSHHIS; -.
DR OrthoDB; 1487552at2759; -.
DR PRO; PR:Q9M0I0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9M0I0; baseline and differential.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:1903428; P:positive regulation of reactive oxygen species biosynthetic process; IGI:TAIR.
DR GO; GO:0072659; P:protein localization to plasma membrane; IGI:TAIR.
DR GO; GO:0080092; P:regulation of pollen tube growth; IGI:TAIR.
DR InterPro; IPR039307; LORELEI-like.
DR PANTHER; PTHR31533; PTHR31533; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein;
KW Membrane; Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..137
FT /note="GPI-anchored protein LLG3"
FT /evidence="ECO:0000255"
FT /id="PRO_5008180242"
FT PROPEP 138..160
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000438102"
FT LIPID 137
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1..47
FT /note="MKITHHCLVSLLSILLLSGFAFSHHISLDEFESHPSTSRALLQAKAT -> M
FT IISLISKIFHDHYVNDILTA (in isoform 2)"
FT /id="VSP_058609"
SQ SEQUENCE 160 AA; 17575 MW; BA7524DCBF64288F CRC64;
MKITHHCLVS LLSILLLSGF AFSHHISLDE FESHPSTSRA LLQAKATCKE DFAAKNYTII
TSKCKGPNYP AKVCCSAFKD FACPFAEVLN DEKTDCASTM FSYINLYGRY PPGIFANMCK
EGKEGLDCTD VTPTSSSHAS IPLVSTHVLL ITVSILFHLF
