LLPH_HUMAN
ID LLPH_HUMAN Reviewed; 129 AA.
AC Q9BRT6; Q3B766;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein LLP homolog;
DE AltName: Full=Protein LAPS18-like;
GN Name=LLPH; Synonyms=C12orf31, cPERP-G {ECO:0000303|PubMed:20813266};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney, and Skeletal muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=20813266; DOI=10.1016/j.cell.2010.07.047;
RA Ohta S., Bukowski-Wills J.C., Sanchez-Pulido L., Alves Fde L., Wood L.,
RA Chen Z.A., Platani M., Fischer L., Hudson D.F., Ponting C.P., Fukagawa T.,
RA Earnshaw W.C., Rappsilber J.;
RT "The protein composition of mitotic chromosomes determined using
RT multiclassifier combinatorial proteomics.";
RL Cell 142:810-821(2010).
RN [4]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-67 AND LYS-74, AND IDENTIFICATION
RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25218447; DOI=10.1038/nsmb.2890;
RA Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M.,
RA Vertegaal A.C.;
RT "Uncovering global SUMOylation signaling networks in a site-specific
RT manner.";
RL Nat. Struct. Mol. Biol. 21:927-936(2014).
RN [5]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-67, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25755297; DOI=10.1074/mcp.o114.044792;
RA Xiao Z., Chang J.G., Hendriks I.A., Sigurdsson J.O., Olsen J.V.,
RA Vertegaal A.C.;
RT "System-wide analysis of SUMOylation dynamics in response to replication
RT stress reveals novel small ubiquitin-like modified target proteins and
RT acceptor lysines relevant for genome stability.";
RL Mol. Cell. Proteomics 14:1419-1434(2015).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-67 AND LYS-74, AND IDENTIFICATION
RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
RN [7] {ECO:0007744|PDB:6LSS, ECO:0007744|PDB:6LU8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.13 ANGSTROMS) IN COMPLEX WITH THE 60S
RP PRE-RIBOSOME.
RX PubMed=32669547; DOI=10.1038/s41467-020-17237-x;
RA Liang X., Zuo M.Q., Zhang Y., Li N., Ma C., Dong M.Q., Gao N.;
RT "Structural snapshots of human pre-60S ribosomal particles before and after
RT nuclear export.";
RL Nat. Commun. 11:3542-3542(2020).
CC -!- FUNCTION: In hippocampal neurons, regulates dendritic and spine growth
CC and synaptic transmission. {ECO:0000250|UniProtKB:Q9D945}.
CC -!- SUBUNIT: Interacts with CTCF, MYO1C and with the transcriptional
CC machinery, including RNA polymerase II and TBP.
CC {ECO:0000250|UniProtKB:Q9D945}.
CC -!- INTERACTION:
CC Q9BRT6; A0A494C108: KRI1; NbExp=3; IntAct=EBI-741396, EBI-12342761;
CC Q9BRT6; Q8N8Y5: ZFP41; NbExp=3; IntAct=EBI-741396, EBI-12224489;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9D945}. Chromosome
CC {ECO:0000269|PubMed:20813266}. Note=Cell-permeable protein. 22 hours
CC after injection in the hippocampal area CA1, internalized by most cells
CC at the injection site (By similarity). Localizes at the chromosome
CC periphery during mitosis (PubMed:20813266).
CC {ECO:0000250|UniProtKB:Q9D945, ECO:0000269|PubMed:20813266}.
CC -!- SIMILARITY: Belongs to the learning-associated protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK091568; BAC03699.1; -; mRNA.
DR EMBL; BC006002; AAH06002.1; -; mRNA.
DR EMBL; BC070232; AAH70232.1; -; mRNA.
DR EMBL; BC107780; AAI07781.1; -; mRNA.
DR CCDS; CCDS8974.1; -.
DR RefSeq; NP_115714.1; NM_032338.3.
DR PDB; 6LSS; EM; 3.23 A; z=1-129.
DR PDB; 6LU8; EM; 3.13 A; z=1-129.
DR PDBsum; 6LSS; -.
DR PDBsum; 6LU8; -.
DR AlphaFoldDB; Q9BRT6; -.
DR SMR; Q9BRT6; -.
DR BioGRID; 124024; 123.
DR IntAct; Q9BRT6; 22.
DR STRING; 9606.ENSP00000266604; -.
DR GlyGen; Q9BRT6; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9BRT6; -.
DR MetOSite; Q9BRT6; -.
DR PhosphoSitePlus; Q9BRT6; -.
DR BioMuta; LLPH; -.
DR DMDM; 74732927; -.
DR EPD; Q9BRT6; -.
DR jPOST; Q9BRT6; -.
DR MassIVE; Q9BRT6; -.
DR MaxQB; Q9BRT6; -.
DR PaxDb; Q9BRT6; -.
DR PeptideAtlas; Q9BRT6; -.
DR PRIDE; Q9BRT6; -.
DR ProteomicsDB; 78829; -.
DR Antibodypedia; 53204; 108 antibodies from 19 providers.
DR DNASU; 84298; -.
DR Ensembl; ENST00000266604.7; ENSP00000266604.2; ENSG00000139233.7.
DR Ensembl; ENST00000446587.2; ENSP00000437372.1; ENSG00000139233.7.
DR GeneID; 84298; -.
DR KEGG; hsa:84298; -.
DR MANE-Select; ENST00000266604.7; ENSP00000266604.2; NM_032338.4; NP_115714.1.
DR UCSC; uc010ssw.3; human.
DR CTD; 84298; -.
DR DisGeNET; 84298; -.
DR GeneCards; LLPH; -.
DR HGNC; HGNC:28229; LLPH.
DR HPA; ENSG00000139233; Low tissue specificity.
DR MIM; 616998; gene.
DR neXtProt; NX_Q9BRT6; -.
DR OpenTargets; ENSG00000139233; -.
DR PharmGKB; PA164722116; -.
DR VEuPathDB; HostDB:ENSG00000139233; -.
DR eggNOG; KOG4811; Eukaryota.
DR GeneTree; ENSGT00390000012979; -.
DR HOGENOM; CLU_134502_0_0_1; -.
DR InParanoid; Q9BRT6; -.
DR OMA; PVWMNPR; -.
DR OrthoDB; 1594400at2759; -.
DR PhylomeDB; Q9BRT6; -.
DR TreeFam; TF314654; -.
DR PathwayCommons; Q9BRT6; -.
DR SignaLink; Q9BRT6; -.
DR BioGRID-ORCS; 84298; 186 hits in 1005 CRISPR screens.
DR ChiTaRS; LLPH; human.
DR GenomeRNAi; 84298; -.
DR Pharos; Q9BRT6; Tbio.
DR PRO; PR:Q9BRT6; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9BRT6; protein.
DR Bgee; ENSG00000139233; Expressed in adrenal tissue and 166 other tissues.
DR ExpressionAtlas; Q9BRT6; baseline and differential.
DR Genevisible; Q9BRT6; HS.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0001099; F:basal RNA polymerase II transcription machinery binding; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0097484; P:dendrite extension; ISS:UniProtKB.
DR GO; GO:0060999; P:positive regulation of dendritic spine development; ISS:UniProtKB.
DR InterPro; IPR018784; LAPS18-like.
DR PANTHER; PTHR34253; PTHR34253; 1.
DR Pfam; PF10169; Laps; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromosome; Isopeptide bond; Nucleus; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..129
FT /note="Protein LLP homolog"
FT /id="PRO_0000274346"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..120
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 67
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25218447,
FT ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733"
FT CROSSLNK 74
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25218447,
FT ECO:0007744|PubMed:28112733"
FT CONFLICT 76
FT /note="E -> G (in Ref. 2; AAI07781)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 129 AA; 15225 MW; 71C2CE6F79B63E4D CRC64;
MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE
KMQCEVKDEK DDMKMETDIK RNKKTLLDQH GQYPIWMNQR QRKRLKAKRE KRKGKSKAKA
VKVAKGLAW
