ID   LMBC1_LUMRU             Reviewed;          76 AA.
AC   O96447;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Antimicrobial peptide lumbricin-1 {ECO:0000312|EMBL:AAC64517.1};
DE   Flags: Precursor;
OS   Lumbricus rubellus (Humus earthworm).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Oligochaeta; Crassiclitellata; Lumbricina; Lumbricidae; Lumbricinae;
OC   Lumbricus.
OX   NCBI_TaxID=35632;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAC64517.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 15-36, FUNCTION,
RP   DEVELOPMENTAL STAGE, INDUCTION, AND MASS SPECTROMETRY.
RX   PubMed=9784609; DOI=10.1016/s0925-4439(98)00058-1;
RA   Cho J.H., Park C.B., Yoon Y.G., Kim S.C.;
RT   "Lumbricin I, a novel proline-rich antimicrobial peptide from the
RT   earthworm: purification, cDNA cloning and molecular characterization.";
RL   Biochim. Biophys. Acta 1408:67-76(1998).
CC   -!- FUNCTION: Displays antimicrobial activity against the Gram-positive
CC       bacteria B.subtilis ATCC 62037, S.aureus ATCC 15752 and S.mutans ATCC
CC       25175, the Gram-negative bacteria E.coli ATCC 27325, P.putida ATCC
CC       17426 and Serratia sp. ATCC 21074, and the fungi C.albicans ATCC 10231,
CC       C.neoformans ATCC 34881 and S.cerevisiae ATCC 44774. Does not possess
CC       hemolytic activity. {ECO:0000269|PubMed:9784609}.
CC   -!- DEVELOPMENTAL STAGE: Adult-specific. Not detected in eggs or 1-week-old
CC       worms but abundant in 6-month-old worms. {ECO:0000269|PubMed:9784609}.
CC   -!- INDUCTION: Constitutively expressed. Not induced by bacterial
CC       infection. {ECO:0000269|PubMed:9784609}.
CC   -!- MASS SPECTROMETRY: Mass=7231; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:9784609};
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DR   EMBL; AF060552; AAC64517.1; -; mRNA.
DR   AlphaFoldDB; O96447; -.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide.
FT   PROPEP          1..14
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000269|PubMed:9784609"
FT                   /id="PRO_0000389427"
FT   PEPTIDE         15..76
FT                   /note="Antimicrobial peptide lumbricin-1"
FT                   /evidence="ECO:0000269|PubMed:9784609"
FT                   /id="PRO_0000389428"
SQ   SEQUENCE   76 AA;  8849 MW;  7D05F906A2B1E09B CRC64;
     MSLCISDYLY LTLTFSKYER QKDKRPYSER KNQYTGPQFL YPPERIPPQK VIKWNEEGLP
     IYEIPGEGGH AEPAAA