LMBD1_DICDI
ID LMBD1_DICDI Reviewed; 510 AA.
AC Q54KD1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Probable lysosomal cobalamin transporter;
DE AltName: Full=LMBR1 domain-containing protein 1 homolog;
GN Name=lmbrd1; ORFNames=DDB_G0287405;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Probable lysosomal cobalamin transporter. Required to export
CC cobalamin from lysosomes allowing its conversion to cofactors (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LIMR family. LMBRD1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AAFI02000100; EAL63770.1; -; Genomic_DNA.
DR RefSeq; XP_637292.1; XM_632200.1.
DR AlphaFoldDB; Q54KD1; -.
DR SMR; Q54KD1; -.
DR STRING; 44689.DDB0235221; -.
DR PaxDb; Q54KD1; -.
DR EnsemblProtists; EAL63770; EAL63770; DDB_G0287405.
DR GeneID; 8626124; -.
DR KEGG; ddi:DDB_G0287405; -.
DR dictyBase; DDB_G0287405; -.
DR eggNOG; ENOG502QQ2T; Eukaryota.
DR HOGENOM; CLU_028341_1_0_1; -.
DR InParanoid; Q54KD1; -.
DR OMA; IITTFTW; -.
DR PhylomeDB; Q54KD1; -.
DR Reactome; R-DDI-9758881; Uptake of dietary cobalamins into enterocytes.
DR Reactome; R-DDI-9758890; Transport of RCbl within the body.
DR PRO; PR:Q54KD1; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR GO; GO:0072665; P:protein localization to vacuole; IBA:GO_Central.
DR InterPro; IPR006876; LMBR1-like_membr_prot.
DR Pfam; PF04791; LMBR1; 1.
PE 3: Inferred from homology;
KW Cobalamin; Cobalt; Lysosome; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..510
FT /note="Probable lysosomal cobalamin transporter"
FT /id="PRO_0000328537"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 22..40
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..129
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..289
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 311..345
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 367..395
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..465
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 487..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 510 AA; 56931 MW; D4C597894DAC3C25 CRC64;
MFPISVGWIA FAGVLLTLIA LNYFIVKYYS DKHESGKITT IISVIGLTLT LLCVMLIPVD
ILNVSTMSHS DGTQISPYSI SQRTEGVKML YYILYGSILG VSLILVPFAY FYHEEYDENI
PTCSRVYAGC KFTIFFILFT IILLVVGAFV RPGSKPIDDQ NVKDWINDEV LNQNAIESSV
LFAISCLTVL GFLVFAVYTS YGLSAFPIGL IKGKKRTDDD KSDINKDLWK AKEKSSFYSS
KYASGKSLSE KEQSTLSLLR GKERALSKRS ERLDNSNKGF RKILVIFRPF AFIFGFIFIL
VSLLIIISIV LSLVDKISSS VCGSACGFLS TYPQLKNPFD IILTKLAPYF PLDYIIIGGL
IFFIYSCTLS GITRIGIRFL WINMFEFGYR KTFPQGLLLA SVLLMLSNLC LNMQIVNLAP
RYVMYGAQVY LNGTSVIPCT INAPTDLCIM SEIGLLTSRI QLGISFFGIV FYYGTWVIVA
TFILGLAVSI FKRRPSAALS YSGDSDEEEI
