ID   LMBD1_NEMVE             Reviewed;         546 AA.
AC   A7RM45;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Probable lysosomal cobalamin transporter;
GN   ORFNames=v1g160387;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CH2 X CH6;
RX   PubMed=17615350; DOI=10.1126/science.1139158;
RA   Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA   Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA   Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA   Technau U., Martindale M.Q., Rokhsar D.S.;
RT   "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT   genomic organization.";
RL   Science 317:86-94(2007).
CC   -!- FUNCTION: Probable lysosomal cobalamin transporter. Required to export
CC       cobalamin from lysosomes allowing its conversion to cofactors (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LIMR family. LMBRD1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; DS469519; EDO47438.1; -; Genomic_DNA.
DR   RefSeq; XP_001639501.1; XM_001639451.1.
DR   AlphaFoldDB; A7RM45; -.
DR   SMR; A7RM45; -.
DR   STRING; 45351.EDO47438; -.
DR   EnsemblMetazoa; EDO47438; EDO47438; NEMVEDRAFT_v1g160387.
DR   GeneID; 5519625; -.
DR   KEGG; nve:5519625; -.
DR   eggNOG; ENOG502QQ2T; Eukaryota.
DR   HOGENOM; CLU_028341_1_0_1; -.
DR   InParanoid; A7RM45; -.
DR   OMA; IITTFTW; -.
DR   OrthoDB; 744771at2759; -.
DR   PhylomeDB; A7RM45; -.
DR   Proteomes; UP000001593; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR   GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR   GO; GO:0072665; P:protein localization to vacuole; IBA:GO_Central.
DR   InterPro; IPR006876; LMBR1-like_membr_prot.
DR   Pfam; PF04791; LMBR1; 1.
PE   3: Inferred from homology;
KW   Cobalamin; Cobalt; Glycoprotein; Lysosome; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..546
FT                   /note="Probable lysosomal cobalamin transporter"
FT                   /id="PRO_0000365833"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        495..515
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        167
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        444
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        452
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        459
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   546 AA;  62686 MW;  A8133D0470B83A54 CRC64;
     MTIPHEVLAQ GWIPFTVVVV LAILFSWFYI RYYQDHSQSE VSSTITGIIA LFIALMTTAL
     VPVDIFLVSY MKNDDGSWKP WSANRMNRDD IEETAVATTY YILYALLAFF VFVIIPFMYF
     FFEERDEDIT TAERACGALK YSIGFLIVAS VLLLVGAFAP LKQPPKNVTE WDKRLIFLKD
     ELKSNNGETA LSLLIGFLTL IGMLIMITYT AYGMTALPFS LLKGFKSAKT EQESVTRRRS
     RNQEQARLIK AQYMGGRAMS SRDRRRLSEL EKEEHALATR ERRLEAAQLG WLNKCLKLLR
     PFEMVFGAFF LLVALLIFVS LFITCLDKAL HSNGYQYGYS LPTPQLPNPI NIIMVYAQIV
     FPLDYCLFLL VVLYFVYSSM AGIRRVGIRC CWIKLFKVRP RRTLPQALLF MCVMLMLIVL
     SLNVMLFSLA PQYVMYGSQN YRANSTVTSL VNHTGIIQNV TTSHGIAKVC SMEMSSDHCT
     MTRIAVFLNR FFYKVWFFGA CYYWGTWLFL VVFMTGLIYS IVRKRKTVVD EEYDSSDDSD
     EEMVTA