LMBD2_MOUSE
ID LMBD2_MOUSE Reviewed; 694 AA.
AC Q8C561; Q3U5C1; Q501M6; Q8BQ06;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=G-protein coupled receptor-associated protein LMBRD2 {ECO:0000250|UniProtKB:Q68DH5};
DE AltName: Full=LMBR1 domain-containing protein 2 {ECO:0000312|MGI:MGI:2444173};
GN Name=Lmbrd2 {ECO:0000312|MGI:MGI:2444173};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion, Thymus, and Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Embryonic germ cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Recruited to ligand-activated beta-2 adrenergic
CC receptor/ADRB2, it negatively regulates the adrenergic receptor
CC signaling pathway. May also regulate other G-protein coupled receptors
CC including type-1 angiotensin II receptor/AGTR1.
CC {ECO:0000250|UniProtKB:Q68DH5}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q68DH5};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8C561-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C561-2; Sequence=VSP_027569;
CC Name=3;
CC IsoId=Q8C561-3; Sequence=VSP_027568;
CC -!- SIMILARITY: Belongs to the LIMR family. {ECO:0000305}.
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DR EMBL; AK051777; BAC34764.1; -; mRNA.
DR EMBL; AK079450; BAC37649.1; -; mRNA.
DR EMBL; AK153728; BAE32158.1; -; mRNA.
DR EMBL; BC095971; AAH95971.1; -; mRNA.
DR EMBL; BC120727; AAI20728.1; -; mRNA.
DR EMBL; BC125421; AAI25422.1; -; mRNA.
DR CCDS; CCDS37039.1; -. [Q8C561-1]
DR RefSeq; NP_796152.2; NM_177178.3. [Q8C561-1]
DR RefSeq; XP_006520154.1; XM_006520091.3. [Q8C561-1]
DR AlphaFoldDB; Q8C561; -.
DR SMR; Q8C561; -.
DR STRING; 10090.ENSMUSP00000087858; -.
DR GlyGen; Q8C561; 1 site.
DR iPTMnet; Q8C561; -.
DR PhosphoSitePlus; Q8C561; -.
DR SwissPalm; Q8C561; -.
DR EPD; Q8C561; -.
DR MaxQB; Q8C561; -.
DR PaxDb; Q8C561; -.
DR PeptideAtlas; Q8C561; -.
DR PRIDE; Q8C561; -.
DR ProteomicsDB; 286211; -. [Q8C561-1]
DR ProteomicsDB; 286212; -. [Q8C561-2]
DR ProteomicsDB; 286213; -. [Q8C561-3]
DR Antibodypedia; 2569; 16 antibodies from 11 providers.
DR DNASU; 320506; -.
DR Ensembl; ENSMUST00000090380; ENSMUSP00000087858; ENSMUSG00000039704. [Q8C561-2]
DR Ensembl; ENSMUST00000227556; ENSMUSP00000154020; ENSMUSG00000039704. [Q8C561-1]
DR GeneID; 320506; -.
DR KEGG; mmu:320506; -.
DR UCSC; uc007vfj.1; mouse. [Q8C561-1]
DR UCSC; uc011zre.1; mouse. [Q8C561-2]
DR CTD; 92255; -.
DR MGI; MGI:2444173; Lmbrd2.
DR VEuPathDB; HostDB:ENSMUSG00000039704; -.
DR eggNOG; KOG2296; Eukaryota.
DR GeneTree; ENSGT00390000018651; -.
DR HOGENOM; CLU_018886_0_0_1; -.
DR InParanoid; Q8C561; -.
DR OMA; ERICYSA; -.
DR OrthoDB; 778022at2759; -.
DR PhylomeDB; Q8C561; -.
DR TreeFam; TF314938; -.
DR BioGRID-ORCS; 320506; 5 hits in 73 CRISPR screens.
DR ChiTaRS; Lmbrd2; mouse.
DR PRO; PR:Q8C561; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8C561; protein.
DR Bgee; ENSMUSG00000039704; Expressed in CA1 field of hippocampus and 235 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071875; P:adrenergic receptor signaling pathway; ISS:UniProtKB.
DR InterPro; IPR006876; LMBR1-like_membr_prot.
DR Pfam; PF04791; LMBR1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Coiled coil; Glycoprotein; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..694
FT /note="G-protein coupled receptor-associated protein
FT LMBRD2"
FT /id="PRO_0000299162"
FT TOPO_DOM 1..3
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 22..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..105
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..150
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..186
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..387
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 409..432
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..473
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 495..521
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 522..542
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 543..694
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 582..638
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..680
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 228..260
FT /evidence="ECO:0000255"
FT COILED 571..603
FT /evidence="ECO:0000255"
FT COMPBIAS 582..621
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 622..638
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 633
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68DH5"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..282
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027568"
FT VAR_SEQ 374..418
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027569"
FT CONFLICT 448
FT /note="V -> L (in Ref. 1; BAE32158)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 694 AA; 81101 MW; 3260F6FB19179DCB CRC64;
MSGAALGLEI VFVFFLALFL LHRYGDFKKQ HRLVIIGTLL AWYLCFLIVF ILPLDVSTTI
YNRCRHAAAN SSPPENTNVT GLDASVTPAP RQHPCFKPWS YIPDGIMPIF WRVVYWTSQF
LTWILLPFMQ SYARSGGFSI TGKIKTALIE NAIYYGTYLL IFGAFLIYVA VNPRLHLEWN
QLQTIGIAAA NTWGLFLLVL LLGYGLVEIP RSYWNGAKRG YLLMKTYFKA AKLMTEKADA
EENLEDVMEE VRKVNESIKY NHPLRKCVDT ILKKCPTDYQ EKMGRNMDDY EDFDEKRNTY
PSEKSLVKLH KQVIYSVQRH RRTQVQWQIL LEQAFYLEDV AKNETSATHQ FVHTFQSPEP
ENRFIQYFYN PTVEWYWECL LRPWFHRTLA VVLSIFSVIV VWSECTFFST TPVLSLFAVF
IQLAERTYNY IYIEIACFLS IFFLSICVYS TVFRIRVFNY YYLASHHQTD AYSLLFSGML
FCRLTPPLCL NFLGLTHMDS SISHQNTQPT AYTSIMGSMK VLSFIADGFY IYYPMLVVIL
CIATYFSLGT RCLNLLGFQQ FMGDNDMTSD LVDEGKELIR REKRKRQRQE EGENRRREWK
ERYGHNREDS TRNRNVHPDP KESNFSDTTT NWSSKYTRAN NRTERDRIEL LQDAEPLDFN
AETFTDESLE PESGRYQPGG RYLSMSSSII FEDI
