LMBL4_MOUSE
ID LMBL4_MOUSE Reviewed; 621 AA.
AC B1B1A0; B2RVB2; Q8BHZ8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Lethal(3)malignant brain tumor-like protein 4;
DE Short=L(3)mbt-like protein 4;
GN Name=L3mbtl4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Putative Polycomb group (PcG) protein. PcG proteins maintain
CC the transcriptionally repressive state of genes, probably via a
CC modification of chromatin, rendering it heritably changed in its
CC expressibility (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B1B1A0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B1B1A0-2; Sequence=VSP_033865, VSP_033866;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK039470; BAC30360.1; -; mRNA.
DR EMBL; AC154493; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC191933; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC117803; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154758; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT010483; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC147118; AAI47119.1; -; mRNA.
DR EMBL; BC147119; AAI47120.1; -; mRNA.
DR RefSeq; NP_796252.2; NM_177278.5. [B1B1A0-1]
DR AlphaFoldDB; B1B1A0; -.
DR SMR; B1B1A0; -.
DR STRING; 10090.ENSMUSP00000094892; -.
DR PhosphoSitePlus; B1B1A0; -.
DR PaxDb; B1B1A0; -.
DR PRIDE; B1B1A0; -.
DR ProteomicsDB; 290042; -. [B1B1A0-1]
DR ProteomicsDB; 290043; -. [B1B1A0-2]
DR DNASU; 320858; -.
DR GeneID; 320858; -.
DR KEGG; mmu:320858; -.
DR UCSC; uc008dkk.2; mouse. [B1B1A0-2]
DR UCSC; uc008dkm.2; mouse. [B1B1A0-1]
DR CTD; 91133; -.
DR MGI; MGI:2444889; L3mbtl4.
DR eggNOG; KOG3766; Eukaryota.
DR InParanoid; B1B1A0; -.
DR OrthoDB; 63195at2759; -.
DR BioGRID-ORCS; 320858; 3 hits in 69 CRISPR screens.
DR ChiTaRS; L3mbtl4; mouse.
DR PRO; PR:B1B1A0; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; B1B1A0; protein.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR004092; Mbt.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR002515; Znf_C2H2C.
DR Pfam; PF02820; MBT; 3.
DR Pfam; PF00536; SAM_1; 1.
DR Pfam; PF01530; zf-C2HC; 1.
DR SMART; SM00561; MBT; 3.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS51079; MBT; 3.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
DR PROSITE; PS51802; ZF_CCHHC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromatin regulator; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..621
FT /note="Lethal(3)malignant brain tumor-like protein 4"
FT /id="PRO_0000337078"
FT REPEAT 52..152
FT /note="MBT 1"
FT REPEAT 160..260
FT /note="MBT 2"
FT REPEAT 269..364
FT /note="MBT 3"
FT DOMAIN 543..607
FT /note="SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00184"
FT ZN_FING 370..414
FT /note="CCHHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 379
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 384
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 398
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT BINDING 404
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01143"
FT VAR_SEQ 154..173
FT /note="DYRKDKFVWMDYLKACRLQN -> GKPGHIYHLQGGVLVIPALF (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_033865"
FT VAR_SEQ 174..621
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_033866"
SQ SEQUENCE 621 AA; 70898 MW; 2F448B5D96E6738F CRC64;
MRQPNRKRKL SLESTERMNQ DRCTGQTEEE KKPGEVTTPS KRESSVTTAE TWSWEQYLRE
GNAVAAPVEL FSKDQSFPEH ENGFQVGMRL EGIDARRPSV FCVLSVAEVC GYRLRLHFDG
YLSCYDFWTN AGSPDIHPVG WCQKTKHELH IPRDYRKDKF VWMDYLKACR LQNAPKKLFR
NRSSNGPVPR EFQVGMKLEA VDRRNPCLMC VATIADIVED RVRVHFDSLD DSFDYWCDVN
SPYIQPVGWC QENGRTLVAP QGYPHPDKFS WTDYLRASQS KAVPAKAFGM RTPHGFLPNM
KLEAVDKRNP QLIRVATIAD VDDYRVKIHF DGWDHKYDYW VDADSQDIHP IGWCDVTGHP
LEVPYGSKHV KILPGQPACP TPGCRGIGHI RGPRYAGHHS AFGCPYSDVN LKREAALQDR
LREQTQANLE LDPSHSKSEN PCNLNVNGKC ENANSQCRLV QQAKCLKIKG KEDIDLDNLF
RVLVFHPRRL EYSAAQVQQM LHQPLSMTST SAHPFRDIPL SREQHCKLLP GVADIQASQV
ARWTVDEVAE FVQSLLGCEE HAKCFKKEQI DGKAFLLLTQ ADIVKVMRIK LGPALKIYNS
ILMFRNSQDV TEDASSQEDK R
