ID   LMBPG_METGU             Reviewed;          73 AA.
AC   P86929;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   03-AUG-2022, entry version 15.
DE   RecName: Full=Antimicrobial peptide lumbricin-PG;
DE   AltName: Full=Lumbricin-PG {ECO:0000303|PubMed:21539875};
DE   Flags: Precursor;
OS   Metaphire guillelmi (Earthworm) (Pheretima guillelmi).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Oligochaeta; Crassiclitellata; Megascolecida; Megascolecidae; Metaphire.
OX   NCBI_TaxID=437222;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 15-40 AND 55-73, FUNCTION,
RP   SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000269|PubMed:21539875}, and
RC   Skin secretion {ECO:0000269|PubMed:21539875};
RX   PubMed=21539875; DOI=10.1016/j.peptides.2011.04.015;
RA   Li W., Li S., Zhong J., Zhu Z., Liu J., Wang W.;
RT   "A novel antimicrobial peptide from skin secretions of the earthworm,
RT   Pheretima guillelmi (Michaelsen).";
RL   Peptides 32:1146-1150(2011).
CC   -!- FUNCTION: Displays antimicrobial activity against the Gram-positive
CC       bacterium S.aureus ATCC 2592, the Gram-negative bacteria E.coli ATCC
CC       25922 and P.aeruginosa ATCC 27853, and the fungus C.albicans ATCC 2002.
CC       Displays stronger activity against P.aeruginosa and S.aureus than
CC       E.coli. Displays very weak hemolytic activity.
CC       {ECO:0000269|PubMed:21539875}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21539875}.
CC   -!- MASS SPECTROMETRY: Mass=6909.07; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21539875};
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DR   AlphaFoldDB; P86929; -.
DR   PRIDE; P86929; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Fungicide;
KW   Hemolysis; Secreted; Signal.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000269|PubMed:21539875"
FT   PEPTIDE         15..73
FT                   /note="Antimicrobial peptide lumbricin-PG"
FT                   /id="PRO_0000413068"
FT   REGION          25..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   73 AA;  8505 MW;  EF17ED4335B86B4E CRC64;
     MLLTISDFLF LSLTFSRYAR MRDSRPWSDR KNNYSGPQFT YPPEKAPPEK LIKWNNEGSP
     IFEMPAEGGH IEP