LMDH_PSEPU
ID LMDH_PSEPU Reviewed; 308 AA.
AC P86808;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 25-MAY-2022, entry version 21.
DE RecName: Full=Limonin dehydrogenase {ECO:0000303|Ref.1};
DE EC=1.2.1.-;
DE Flags: Fragments;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, ACTIVITY REGULATION,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC Biotype A;
RA Ghosh M., Malik M.;
RT "Enhancement of bioconversion of limonin by P.putida G7.";
RL Submitted (SEP-2010) to UniProtKB.
CC -!- FUNCTION: Catalyzes the NAD(+)-dependent conversion of limonin.
CC {ECO:0000269|Ref.1}.
CC -!- ACTIVITY REGULATION: Completely inhibited by HgCl(2), CoCl(2) and
CC CaCl(2). {ECO:0000269|Ref.1}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=4.5 uM for limonin {ECO:0000269|Ref.1};
CC pH dependence:
CC Optimum pH is 8.5. {ECO:0000269|Ref.1};
CC Temperature dependence:
CC Optimum temperature is 35 degrees Celsius. {ECO:0000269|Ref.1};
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000255}.
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DR AlphaFoldDB; P86808; -.
DR SMR; P86808; -.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.605.10; -; 1.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR Pfam; PF00171; Aldedh; 1.
DR SUPFAM; SSF53720; SSF53720; 1.
PE 1: Evidence at protein level;
KW NAD; Oxidoreductase; Periplasm.
FT CHAIN <1..>308
FT /note="Limonin dehydrogenase"
FT /id="PRO_0000406597"
FT NON_CONS 14..15
FT /evidence="ECO:0000305"
FT NON_CONS 28..29
FT /evidence="ECO:0000305"
FT NON_CONS 46..47
FT /evidence="ECO:0000305"
FT NON_CONS 63..64
FT /evidence="ECO:0000305"
FT NON_CONS 69..70
FT /evidence="ECO:0000305"
FT NON_CONS 83..84
FT /evidence="ECO:0000305"
FT NON_CONS 94..95
FT /evidence="ECO:0000305"
FT NON_CONS 115..116
FT /evidence="ECO:0000305"
FT NON_CONS 135..136
FT /evidence="ECO:0000305"
FT NON_CONS 151..152
FT /evidence="ECO:0000305"
FT NON_CONS 167..168
FT /evidence="ECO:0000305"
FT NON_CONS 176..177
FT /evidence="ECO:0000305"
FT NON_CONS 189..190
FT /evidence="ECO:0000305"
FT NON_CONS 203..204
FT /evidence="ECO:0000305"
FT NON_CONS 209..210
FT /evidence="ECO:0000305"
FT NON_CONS 216..217
FT /evidence="ECO:0000305"
FT NON_CONS 222..223
FT /evidence="ECO:0000305"
FT NON_CONS 231..232
FT /evidence="ECO:0000305"
FT NON_CONS 243..244
FT /evidence="ECO:0000305"
FT NON_CONS 258..259
FT /evidence="ECO:0000305"
FT NON_CONS 276..277
FT /evidence="ECO:0000305"
FT NON_CONS 291..292
FT /evidence="ECO:0000305"
FT NON_CONS 302..303
FT /evidence="ECO:0000305"
FT NON_TER 1
FT NON_TER 308
FT /evidence="ECO:0000303|Ref.1"
SQ SEQUENCE 308 AA; 31977 MW; 1C7D5F7B6E275D66 CRC64;
ALMKTKLFIN NTMASSGIGL ETDAIKAAQA VYCQGQICMS EEQEALCPFD RLEGEVAIVV
GAGPSERRRL LLKVADVMES KTPKFIEVMA MEVGASALWA GFNVHASANV FREAASLATQ
IQGTPLGFAV PTEAFEMATP DGTGALNYGV RRPKGVIGVI SPWNLPLLLM TWKVGPALAC
GNTVVVKPSR INGLFKDAID KGAKVVCGGM AQGAVGVLNY LNRVNAVQPG GTVISLCGPA
SAGFDIANDS VYGLSSGDDM IKQLTPLGFA AKPEDNVEPY LLGASRKQGK GITTTVISID
GGMALGAG
