ID   LMLN_CAEEL              Reviewed;         663 AA.
AC   O62446;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Leishmanolysin-like peptidase {ECO:0000305};
DE            EC=3.4.24.- {ECO:0000250|UniProtKB:Q9VH19};
GN   ORFNames=Y43F4A.1;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Essential for the coordination of mitotic progression, and
CC       also plays a role in cell migration. {ECO:0000250|UniProtKB:Q9VH19}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:P08148};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P08148};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Found in ring-like structures
CC       resembling invadopodia. In migrating cells it relocalizes from internal
CC       structures to the leading edge of cells (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M8 family. {ECO:0000305}.
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DR   EMBL; Z99271; CAB16471.1; -; Genomic_DNA.
DR   PIR; T26835; T26835.
DR   RefSeq; NP_001022870.1; NM_001027699.3.
DR   AlphaFoldDB; O62446; -.
DR   SMR; O62446; -.
DR   STRING; 6239.Y43F4A.1a; -.
DR   PaxDb; O62446; -.
DR   EnsemblMetazoa; Y43F4A.1a.1; Y43F4A.1a.1; WBGene00012796.
DR   GeneID; 176737; -.
DR   KEGG; cel:CELE_Y43F4A.1; -.
DR   UCSC; Y43F4A.1b; c. elegans.
DR   CTD; 176737; -.
DR   WormBase; Y43F4A.1a; CE16622; WBGene00012796; -.
DR   eggNOG; KOG2556; Eukaryota.
DR   HOGENOM; CLU_023820_1_0_1; -.
DR   InParanoid; O62446; -.
DR   OMA; HSESMWE; -.
DR   OrthoDB; 1310134at2759; -.
DR   PhylomeDB; O62446; -.
DR   PRO; PR:O62446; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00012796; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR   ExpressionAtlas; O62446; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008233; F:peptidase activity; IBA:GO_Central.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR001577; Peptidase_M8.
DR   PANTHER; PTHR10942; PTHR10942; 1.
DR   Pfam; PF01457; Peptidase_M8; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Cytoplasm; Hydrolase; Metal-binding;
KW   Metalloprotease; Mitosis; Protease; Reference proteome; Zinc.
FT   CHAIN           1..663
FT                   /note="Leishmanolysin-like peptidase"
FT                   /id="PRO_0000303079"
FT   ACT_SITE        247
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         246
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         250
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         353
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   663 AA;  76050 MW;  814C317228B5ACEA CRC64;
     MKPRLIFIFF ACYFLNFLPF SNQLPCSYQN PRIEDILLEV PIEHEHPHRH RRGLPSSDPT
     SPPVEKFAPL RIQLHYDKSI QNLTAEVQNF VNTTLLPEAV GYWENALRVR PMTTPIRLRR
     KCISSFYYYK QGMRNVACDK GCRERTTCGE ADIPRDHLLD CLACNNTDDC KTTGELGEGV
     KDTDFILYVT AHDSKRCEGP ETLSYAAHCQ QEADFDRPIA GNVNLCPSAL SVHNHDYEIL
     TSTVKHEILH ALGFSVGLYA FFRDSEGKPR TKRNRYGRPT SLNKQKGYYD WDSNTITTVL
     RENWWTGEGK VIHPIHMMVT PKVREEARRH FGCDKLEGAE LENQGGEGTY LTHWEKRAYE
     NEAMTGTHTQ NPVYSRLTLA FLEDTGWYQP NYEVAEDLHW GKQLGCDFAM KSCGEWIHEK
     KILGEDAYPY CSDIKHDGSK SMAITRCTTQ RDSLALCNLV PFQKELPSQY RNFMSLPGVN
     PDGAKYYGGS VEMADYCPFL QEFEWKLIDK TQHKDSRCEL EGNGKEGEDI LEVYGANSKC
     FEFPKPWTER KCGRIRVLSH YMAGCYEHQC TNGTLYVGSY NATDMYPCYA ENQKIHIKKV
     VDGWLREGSL ICPKCEDYCT NCGPPIVIPD YIGDPELDEP CSSNFKLSVL AFLCYLIFLH
     IRS