LMNB1_DICDI
ID LMNB1_DICDI Reviewed; 716 AA.
AC Q54HI5;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Lamin-like protein {ECO:0000312|dictyBase:DDB_G0289429};
DE AltName: Full=Protein NE81 {ECO:0000303|PubMed:22090348};
GN Name=lmnB {ECO:0000312|dictyBase:DDB_G0289429};
GN ORFNames=DDB_G0289429 {ECO:0000312|EMBL:EAL62742.1};
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2] {ECO:0000305}
RP SUBCELLULAR LOCATION.
RC STRAIN=AX2 {ECO:0000303|PubMed:19466752};
RX PubMed=19466752; DOI=10.1002/cm.20384;
RA Schulz I., Erle A., Graf R., Kruger A., Lohmeier H., Putzler S.,
RA Samereier M., Weidenthaler S.;
RT "Identification and cell cycle-dependent localization of nine novel,
RT genuine centrosomal components in Dictyostelium discoideum.";
RL Cell Motil. Cytoskeleton 66:915-928(2009).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF
RP SER-122 AND 713-CYS--MET-716, AND NUCLEAR LOCALIZATION SIGNAL.
RC STRAIN=AX2 {ECO:0000303|PubMed:22090348};
RX PubMed=22090348; DOI=10.1091/mbc.e11-07-0595;
RA Krueger A., Batsios P., Baumann O., Luckert E., Schwarz H., Stick R.,
RA Meyer I., Graef R.;
RT "Characterization of NE81, the first lamin-like nucleoskeleton protein in a
RT unicellular organism.";
RL Mol. Biol. Cell 23:360-370(2012).
CC -!- FUNCTION: Component of the nuclear lamina, a fibrous layer on the
CC nucleoplasmic side of the inner nuclear membrane. Helps to maintain
CC integrity of nuclear structures in response to mechanical stress.
CC {ECO:0000269|PubMed:22090348}.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:19466752}.
CC Nucleus inner membrane {ECO:0000269|PubMed:22090348}.
CC -!- DISRUPTION PHENOTYPE: Nuclei are misshapen and disorganized, with
CC irregularly condensed chromatin and centrosomal detachment from nuclei.
CC {ECO:0000269|PubMed:22090348}.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR EMBL; AAFI02000140; EAL62742.1; -; Genomic_DNA.
DR RefSeq; XP_636248.1; XM_631156.1.
DR AlphaFoldDB; Q54HI5; -.
DR SMR; Q54HI5; -.
DR STRING; 44689.DDB0304833; -.
DR PaxDb; Q54HI5; -.
DR EnsemblProtists; EAL62742; EAL62742; DDB_G0289429.
DR GeneID; 8627138; -.
DR KEGG; ddi:DDB_G0289429; -.
DR dictyBase; DDB_G0289429; lmnB.
DR eggNOG; ENOG502RE3N; Eukaryota.
DR HOGENOM; CLU_386089_0_0_1; -.
DR InParanoid; Q54HI5; -.
DR OMA; NEFYWSR; -.
DR PhylomeDB; Q54HI5; -.
DR PRO; PR:Q54HI5; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005638; C:lamin filament; IDA:dictyBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:dictyBase.
DR GO; GO:0005637; C:nuclear inner membrane; IDA:dictyBase.
DR GO; GO:0005652; C:nuclear lamina; IDA:dictyBase.
DR GO; GO:0030527; F:structural constituent of chromatin; IMP:dictyBase.
DR GO; GO:0007098; P:centrosome cycle; IMP:dictyBase.
DR GO; GO:0051642; P:centrosome localization; IMP:dictyBase.
DR GO; GO:0010847; P:regulation of chromatin assembly; IMP:dictyBase.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR001322; Lamin_tail_dom.
DR InterPro; IPR036415; Lamin_tail_dom_sf.
DR SUPFAM; SSF74853; SSF74853; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
DR PROSITE; PS51841; LTD; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Intermediate filament; Membrane; Nucleus; Reference proteome.
FT CHAIN 1..716
FT /note="Lamin-like protein"
FT /evidence="ECO:0000305"
FT /id="PRO_0000430572"
FT DOMAIN 132..515
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT DOMAIN 575..698
FT /note="LTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01187"
FT REGION 1..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 519..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 130..450
FT /evidence="ECO:0000255"
FT MOTIF 519..522
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:22090348"
FT MOTIF 713..716
FT /note="CAAX motif"
FT /evidence="ECO:0000303|PubMed:22090348"
FT COMPBIAS 15..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 122
FT /note="S->A: Fails to localize to the nuclear envelope, and
FT instead forms nuclear aggregates which persist throughout
FT mitosis; when associated with deletion of 713-C--M-716."
FT /evidence="ECO:0000269|PubMed:22090348"
FT MUTAGEN 713..716
FT /note="Missing: Fails to localize to the nuclear envelope,
FT and instead forms nuclear aggregates which disperse at the
FT onset of mitosis. Forms nuclear aggregates which persist
FT throughout mitosis; when associated with S-122."
FT /evidence="ECO:0000269|PubMed:22090348"
SQ SEQUENCE 716 AA; 81350 MW; 413546C1FEE1CB0A CRC64;
MDMSKKKSKR ASPIESSQEE IAISTSKTAT TEKPKKTKTT TKKKASQPSQ EVVMETESEV
EITTTTTSTS TTNNNNITTT STSSQQSNGT LSSSSSPTIQ SIPTTPISKY IPSLSQIGTP
LSPNRAAQRL REKDELSLIH NRLKSALKKL ESAETELEKK NQEYEELDQK HTATIKQLKQ
RSDQVEKQLI EEQNQNSDLT SNRNILENEL KSKESVWKKE KDEILLKFQE SINKLNQENS
LAQSQLKSEI VSKEYEIDGL KSEINRLKDD LQYRIREGEE KSRKLLENEY NRFKGKEEEY
NQLIVSKDEE IKKYKFELKE KEKSSNAMNK KENELNNLIQ AHERQIEDMR DSINREWELK
AAQMMEEHHA RTIHLQQAVD SFNEEKERIK SQMETLNGQI EDINIKNNEY EDRIKEMNVL
LSQKDNSIGE LGVEIEESKK KMRKQMADLK SKDGQIALLQ IEINTKDNKC NTLQTETNRL
KSELYSITNQ IDPEIPLDPE INSLKELVKG FEKTVDDRKR KRSKLQHEFN AAANQDQNGM
TIEEQSSTST TTTTSATGSS SSTSHLDNID SSKLPTGPEQ SELFNPDTVS FSLVDSNQEF
IKLSVHGDMD NGLSISKWRL IVVKPDGSKS GFSFPDGIQP FKGIKSVTVW TGRPRPQGTP
TENEFYWART ELWTSPVEGT IVKLVSPSEE TTTVTLPADG IYQKPSSAGK SNCLIM
