ID   LMO4A_XENLA             Reviewed;         171 AA.
AC   Q8AW92;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=LIM domain transcription factor LMO4-A;
DE   AltName: Full=LIM domain only protein 4-A;
DE            Short=LMO-4-A;
DE   AltName: Full=Xlmo4;
GN   Name=lmo4-a; Synonyms=lmo4 {ECO:0000312|EMBL:CAD54077.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAD54077.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND INDUCTION.
RC   TISSUE=Animal cap {ECO:0000269|PubMed:14651938};
RX   PubMed=14651938; DOI=10.1016/j.ydbio.2003.09.002;
RA   de la Calle-Mustienes E., Lu Z., Cortes M., Andersen B., Modolell J.,
RA   Gomez-Skarmeta J.L.;
RT   "Xenopus Xlmo4 is a GATA cofactor during ventral mesoderm formation and
RT   regulates Ldb1 availability at the dorsal mesoderm and the neural plate.";
RL   Dev. Biol. 264:564-581(2003).
RN   [2] {ECO:0000312|EMBL:AAI08586.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula {ECO:0000312|EMBL:AAI08586.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a positive cofactor of GATA transcription factors to
CC       establish the identity of the ventral mesoderm during gastrulation.
CC       Down-regulation in the dorsal mesoderm is necessary for the proper
CC       formation of this territory since, when present, lmo4 may bind ldb1 and
CC       restrict the availability of this cofactor for Spemman organizer
CC       transcription factors. At neurula stages, suppresses primary neuron
CC       differentiation and modulates gene expression at the Isthmic Organizer
CC       of the midbrain-hindbrain boundary. {ECO:0000269|PubMed:14651938}.
CC   -!- INTERACTION:
CC       Q8AW92; O13097: efnb1; NbExp=2; IntAct=EBI-15667028, EBI-15667006;
CC   -!- TISSUE SPECIFICITY: At the start of gastrulation (stage 10), expressed
CC       in the mesodermal marginal zone. Shortly after (stage 11), expression
CC       is down-regulated in the dorsal most region. During neurulation,
CC       expressed in the neural plate and ventral epidermis. At late neurula
CC       stages, also expressed more rostrally, and then in the brain, migrating
CC       neural crests and ventral epidermis. {ECO:0000269|PubMed:14651938}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC       {ECO:0000269|PubMed:14651938}.
CC   -!- INDUCTION: By irx1 in the neural plate. By bmp4-signaling in the
CC       ventral mesoderm. Down-regulated in the dorsal mesoderm at stage 11 by
CC       Spemann Organizer repressors including gsc.
CC       {ECO:0000269|PubMed:14651938}.
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DR   EMBL; AJ511277; CAD54077.1; -; mRNA.
DR   EMBL; BC108585; AAI08586.1; -; mRNA.
DR   RefSeq; NP_001079179.1; NM_001085710.1.
DR   AlphaFoldDB; Q8AW92; -.
DR   SMR; Q8AW92; -.
DR   DIP; DIP-60984N; -.
DR   IntAct; Q8AW92; 1.
DR   DNASU; 373776; -.
DR   GeneID; 373776; -.
DR   KEGG; xla:373776; -.
DR   CTD; 373776; -.
DR   Xenbase; XB-GENE-865262; lmo4.2.L.
DR   OrthoDB; 1307952at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 373776; Expressed in heart and 19 other tissues.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007369; P:gastrulation; IEA:UniProtKB-KW.
DR   GO; GO:0007498; P:mesoderm development; IMP:UniProtKB.
DR   GO; GO:0045665; P:negative regulation of neuron differentiation; IMP:UniProtKB.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00132; LIM; 2.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; Gastrulation; LIM domain;
KW   Metal-binding; Neurogenesis; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..171
FT                   /note="LIM domain transcription factor LMO4-A"
FT                   /id="PRO_0000317221"
FT   DOMAIN          22..84
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          86..148
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   171 AA;  18630 MW;  336973B41BC7AE1C CRC64;
     MVNNRSSEST TTAVSSNGSP PKACAGCGGK IGDRFLLYSM DRYWHTRCLK CSCCQAQLGE
     IGTSCYTKSG MILCRNDYIR LFGNSGACNA CGQSIPASEM VMRAQGSVYH LKCFTCATCR
     NRLVPGDRFH YVNGTIFCEH DRPTGLLNGH LNPLQSNPLQ GSPMLPDQKV C