LMPB_DICDI
ID LMPB_DICDI Reviewed; 755 AA.
AC Q9BKJ9; Q54KX1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Lysosome membrane protein 2-B;
DE AltName: Full=Lysosome membrane protein II-2;
DE Short=LIMP II-2;
GN Name=lmpB; ORFNames=DDB_G0287035;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP GLYCOSYLATION, AND TOPOLOGY.
RC STRAIN=AX4;
RX PubMed=11489884; DOI=10.1074/jbc.m103384200;
RA Janssen K.-P., Rost R., Eichinger L., Schleicher M.;
RT "Characterization of CD36/LIMPII homologues in Dictyostelium discoideum.";
RL J. Biol. Chem. 276:38899-38910(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: May act as a lysosomal receptor (By similarity). May be
CC involved in macropinocytosis and fluid phase exocytosis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Localizes to membranes of
CC endolysosomal vesicles and macropinosomes.
CC {ECO:0000269|PubMed:11489884}.
CC -!- DEVELOPMENTAL STAGE: Shows a bi-phasic developmental regulation.
CC Present in growth phase cells as well as in early aggregating cells,
CC but the levels drop significantly during the slug stage. Protein levels
CC rise again at later multicellular stages of the development to higher
CC amounts than in growth phase cells, and the protein is still detectable
CC in significant amounts in fully developed fruiting bodies (at protein
CC level). {ECO:0000269|PubMed:11489884}.
CC -!- PTM: Heavily glycosylated. {ECO:0000269|PubMed:11489884}.
CC -!- SIMILARITY: Belongs to the CD36 family. {ECO:0000305}.
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DR EMBL; AF238324; AAK30040.1; -; mRNA.
DR EMBL; AAFI02000096; EAL63882.1; -; Genomic_DNA.
DR RefSeq; XP_637399.1; XM_632307.1.
DR AlphaFoldDB; Q9BKJ9; -.
DR STRING; 44689.DDB0191189; -.
DR PaxDb; Q9BKJ9; -.
DR ABCD; Q9BKJ9; 6 sequenced antibodies.
DR EnsemblProtists; EAL63882; EAL63882; DDB_G0287035.
DR GeneID; 8625930; -.
DR KEGG; ddi:DDB_G0287035; -.
DR dictyBase; DDB_G0287035; lmpB.
DR eggNOG; ENOG502RBM0; Eukaryota.
DR HOGENOM; CLU_368981_0_0_1; -.
DR InParanoid; Q9BKJ9; -.
DR OMA; KAIHGRY; -.
DR PhylomeDB; Q9BKJ9; -.
DR Reactome; R-DDI-114608; Platelet degranulation.
DR Reactome; R-DDI-434313; Intracellular metabolism of fatty acids regulates insulin secretion.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-DDI-8856828; Clathrin-mediated endocytosis.
DR PRO; PR:Q9BKJ9; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IDA:dictyBase.
DR GO; GO:0032009; C:early phagosome; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045335; C:phagocytic vesicle; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0031982; C:vesicle; IDA:dictyBase.
DR GO; GO:0012506; C:vesicle membrane; IBA:GO_Central.
DR GO; GO:0005044; F:scavenger receptor activity; IBA:GO_Central.
DR GO; GO:0006911; P:phagocytosis, engulfment; IMP:dictyBase.
DR InterPro; IPR002159; CD36_fam.
DR PANTHER; PTHR11923; PTHR11923; 1.
DR Pfam; PF01130; CD36; 2.
DR PRINTS; PR01609; CD36FAMILY.
PE 1: Evidence at protein level;
KW Glycoprotein; Lysosome; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..755
FT /note="Lysosome membrane protein 2-B"
FT /id="PRO_0000327758"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..727
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 728..748
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 749..755
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 752..753
FT /note="Di-leucine motif"
FT /evidence="ECO:0000255"
FT CARBOHYD 28
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 379
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 465
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 497
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 588
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 607
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 680
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 755 AA; 82572 MW; 93BB639461C97547 CRC64;
MKHIGRIVSF PIGLVLIAVG IIIFVVVNRT IKDEFKKAAV VIPDNGAEEI VDPWVRFIGN
EGDPNNVRTY TFMAYNLTNP IETMQGHLPK YQEVGPYSYN YIYERINANL YEDDEKLSFK
LWKRYFPIVA DGYRDPTKDT IYHFNLVYGA AVKQAGSEVA LSVALTAAAM GKIITGLTDP
SFKVKAGFAA APTVTAGAFS NLLTAAGNDP ATACGLWQTS TSSSTPLVPF SVPIIAGSPS
DISQAQCQAL FDPSNKFSLT DPTNVGVYLL NPAGSKAALL ASPFGLTSVQ ADLIMKYQLA
LTSTFVPTTL VSRFAECSDP KTCTNNPLYF GLLQWAKNPS LLGQSVFAIP NSGVPAAPEF
GIYTSSELSL TKAGSLFLNT STINLITPTS IGTILVYGQK LKADPTSIPP ALYAPFSSGE
FDAIVKYTGY IMAAFVDGDI IKNQIFKDYQ GGPIVKHTVH DFLFNSTDPL LKLMYPDTPS
AWVSSPLDNI QDVKVANATL HTDEIYTGVG DIDLVSSPIT FEGEEELNYN KKIKVSGSFA
EQLPPSYLSK DPEAPVNVFT DEFARSLSFR KEVGGNFGGI PYYRYRINES NWEINPDYFQ
TIPYLLNLTS IKSGAPAYLS RPRLKGIDVG YYYKAGITDL INDDEDLDVF ADYEPRSGKA
IHGRYSLQVN TYIQGSDGTN STLYNKYSAF RSDVVHPMFW GVNIIAATQE QIDILTKAYK
VDSFRYAITV ILIVVGGFLS LISGGLFVLD KIIDL
