LMX1A_MOUSE
ID LMX1A_MOUSE Reviewed; 382 AA.
AC Q9JKU8;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=LIM homeobox transcription factor 1-alpha;
DE AltName: Full=LIM/homeobox protein 1.1;
DE Short=LMX-1.1;
DE AltName: Full=LIM/homeobox protein LMX1A;
GN Name=Lmx1a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C3HeB/Fele-a/a;
RX PubMed=10693804; DOI=10.1038/35001573;
RA Millonig J.H., Millen K.J., Hatten M.E.;
RT "The mouse Dreher gene Lmx1a controls formation of the roof plate in the
RT vertebrate CNS.";
RL Nature 403:764-769(2000).
CC -!- FUNCTION: Acts as a transcriptional activator by binding to an A/T-rich
CC sequence, the FLAT element, in the insulin gene promoter. Required for
CC development of the roof plate and, in turn, for specification of dorsal
CC cell fates in the CNS and developing vertebrae.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- DISEASE: Note=Defects in Lmx1a are the cause of the spontaneous
CC neurologic mutant mouse, called 'dreher' (dr) and results from a
CC failure of the roof plate to develop.
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DR EMBL; AF226662; AAF43012.1; -; mRNA.
DR CCDS; CCDS15460.1; -.
DR RefSeq; NP_387501.1; NM_033652.5.
DR RefSeq; XP_006496674.1; XM_006496611.2.
DR RefSeq; XP_017177173.1; XM_017321684.1.
DR AlphaFoldDB; Q9JKU8; -.
DR SMR; Q9JKU8; -.
DR BioGRID; 225780; 5.
DR IntAct; Q9JKU8; 4.
DR STRING; 10090.ENSMUSP00000028003; -.
DR PhosphoSitePlus; Q9JKU8; -.
DR PaxDb; Q9JKU8; -.
DR PRIDE; Q9JKU8; -.
DR ProteomicsDB; 292345; -.
DR Antibodypedia; 20523; 221 antibodies from 28 providers.
DR DNASU; 110648; -.
DR Ensembl; ENSMUST00000028003; ENSMUSP00000028003; ENSMUSG00000026686.
DR Ensembl; ENSMUST00000111377; ENSMUSP00000107008; ENSMUSG00000026686.
DR GeneID; 110648; -.
DR KEGG; mmu:110648; -.
DR UCSC; uc007dlc.1; mouse.
DR CTD; 4009; -.
DR MGI; MGI:1888519; Lmx1a.
DR VEuPathDB; HostDB:ENSMUSG00000026686; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000157774; -.
DR HOGENOM; CLU_027802_0_0_1; -.
DR InParanoid; Q9JKU8; -.
DR OMA; SXAEPLF; -.
DR OrthoDB; 737134at2759; -.
DR PhylomeDB; Q9JKU8; -.
DR TreeFam; TF315442; -.
DR BioGRID-ORCS; 110648; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Lmx1a; mouse.
DR PRO; PR:Q9JKU8; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9JKU8; protein.
DR Bgee; ENSMUSG00000026686; Expressed in roof plate and 53 other tissues.
DR ExpressionAtlas; Q9JKU8; baseline and differential.
DR Genevisible; Q9JKU8; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:ParkinsonsUK-UCL.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007411; P:axon guidance; IMP:MGI.
DR GO; GO:0007420; P:brain development; ISO:MGI.
DR GO; GO:0007417; P:central nervous system development; IMP:MGI.
DR GO; GO:0021953; P:central nervous system neuron differentiation; IMP:MGI.
DR GO; GO:0021549; P:cerebellum development; IMP:MGI.
DR GO; GO:0021542; P:dentate gyrus development; IMP:MGI.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:MGI.
DR GO; GO:0021766; P:hippocampus development; IMP:MGI.
DR GO; GO:0007626; P:locomotory behavior; IGI:ParkinsonsUK-UCL.
DR GO; GO:0007613; P:memory; IGI:ParkinsonsUK-UCL.
DR GO; GO:0030901; P:midbrain development; IMP:MGI.
DR GO; GO:1904948; P:midbrain dopaminergic neuron differentiation; IDA:ParkinsonsUK-UCL.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR GO; GO:0042048; P:olfactory behavior; IGI:ParkinsonsUK-UCL.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IC:ParkinsonsUK-UCL.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:ParkinsonsUK-UCL.
DR GO; GO:1905426; P:positive regulation of Wnt-mediated midbrain dopaminergic neuron differentiation; IC:ParkinsonsUK-UCL.
DR GO; GO:0001558; P:regulation of cell growth; IMP:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050808; P:synapse organization; IGI:ParkinsonsUK-UCL.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd09370; LIM1_Lmx1a; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR042688; Lmx1a_LIM1.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; LIM domain;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..382
FT /note="LIM homeobox transcription factor 1-alpha"
FT /id="PRO_0000075827"
FT DOMAIN 33..92
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 92..154
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 195..254
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 161..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..195
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 382 AA; 42857 MW; 6C856843FEAC6706 CRC64;
MLDGLKMEEN FQSAIETSAS FSSLLGRAVS PKSVCEGCQR VISDRFLLRL NDSFWHEQCV
QCASCKEPLE TTCFYRDKKL YCKYHYEKLF AVKCGGCFEA IAPNEFVMRA QKSVYHLSCF
CCCVCERQLQ KGDEFVLKEG QLLCKGDYEK ERELLSLVSP AASDSGKSDD EESLCKSAHG
AGKGASEDGK DHKRPKRPRT ILTTQQRRAF KASFEVSSKP CRKVRETLAA ETGLSVRVVQ
VWFQNQRAKM KKLARRQQQQ QQDQQNTQRL TSAQTNGSGN AGMEGIMNPY TTLPTPQQLL
AIEQSVYNSD PFRQGLTPPQ MPGDHMHPYG AEPLFHDLDS DDTSLSNLGD CFLATSEAGP
LQSRVGNPID HLYSMQNSYF TS
