LNEBL_MOUSE
ID LNEBL_MOUSE Reviewed; 270 AA.
AC Q9DC07;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=LIM zinc-binding domain-containing Nebulette;
DE AltName: Full=Actin-binding Z-disk protein;
GN Name=Nebl; Synonyms=Lnebl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION (ISOFORM 2).
RC TISSUE=Placenta;
RX PubMed=15004028; DOI=10.1074/jbc.m310304200;
RA Li B., Zhuang L., Trueb B.;
RT "Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-
RT nebulette and Lasp-1.";
RL J. Biol. Chem. 279:20401-20410(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-135 AND SER-230, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-96 AND ARG-132, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Binds to actin and plays an important role in the assembly of
CC the Z-disk. Isoform 2 might play a role in the assembly of focal
CC adhesion (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms exist.;
CC Name=2; Synonyms=LIM-Nebulette, LIM-NBEL, LNBEL;
CC IsoId=Q9DC07-1; Sequence=Displayed;
CC Name=1;
CC IsoId=Q0II04-1; Sequence=External;
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DR EMBL; AK004645; BAB23436.1; -; mRNA.
DR EMBL; AL935297; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL772218; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL845498; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL954131; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466542; EDL08088.1; -; Genomic_DNA.
DR EMBL; BC025863; AAH25863.1; -; mRNA.
DR CCDS; CCDS15706.1; -. [Q9DC07-1]
DR RefSeq; NP_083033.1; NM_028757.2. [Q9DC07-1]
DR AlphaFoldDB; Q9DC07; -.
DR SMR; Q9DC07; -.
DR BioGRID; 216494; 4.
DR iPTMnet; Q9DC07; -.
DR SwissPalm; Q9DC07; -.
DR jPOST; Q9DC07; -.
DR MaxQB; Q9DC07; -.
DR PaxDb; Q9DC07; -.
DR PeptideAtlas; Q9DC07; -.
DR PRIDE; Q9DC07; -.
DR ProteomicsDB; 252483; -. [Q9DC07-1]
DR Antibodypedia; 2823; 82 antibodies from 18 providers.
DR DNASU; 74103; -.
DR Ensembl; ENSMUST00000028080; ENSMUSP00000028080; ENSMUSG00000053702. [Q9DC07-1]
DR GeneID; 74103; -.
DR KEGG; mmu:74103; -.
DR UCSC; uc008ila.1; mouse. [Q9DC07-1]
DR CTD; 10529; -.
DR MGI; MGI:1921353; Nebl.
DR VEuPathDB; HostDB:ENSMUSG00000053702; -.
DR GeneTree; ENSGT00940000156390; -.
DR HOGENOM; CLU_026811_0_1_1; -.
DR OMA; DHSMERY; -.
DR OrthoDB; 1549538at2759; -.
DR PhylomeDB; Q9DC07; -.
DR BioGRID-ORCS; 74103; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Nebl; mouse.
DR Proteomes; UP000000589; Chromosome 2.
DR Bgee; ENSMUSG00000053702; Expressed in interventricular septum and 195 other tissues.
DR ExpressionAtlas; Q9DC07; baseline and differential.
DR Genevisible; Q9DC07; MM.
DR GO; GO:0001725; C:stress fiber; ISO:MGI.
DR GO; GO:0030018; C:Z disc; ISO:MGI.
DR GO; GO:0051015; F:actin filament binding; ISO:MGI.
DR GO; GO:0008092; F:cytoskeletal protein binding; ISO:MGI.
DR GO; GO:0031005; F:filamin binding; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005523; F:tropomyosin binding; ISO:MGI.
DR GO; GO:0071691; P:cardiac muscle thin filament assembly; ISO:MGI.
DR CDD; cd11935; SH3_Nebulette_C; 1.
DR InterPro; IPR035631; Nebulette_SH3.
DR InterPro; IPR000900; Nebulin_repeat.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00412; LIM; 1.
DR Pfam; PF00880; Nebulin; 2.
DR Pfam; PF14604; SH3_9; 1.
DR PRINTS; PR00452; SH3DOMAIN.
DR SMART; SM00132; LIM; 1.
DR SMART; SM00227; NEBU; 3.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 1.
DR PROSITE; PS51216; NEBULIN; 3.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Alternative splicing; Cytoplasm; LIM domain; Metal-binding;
KW Methylation; Phosphoprotein; Reference proteome; Repeat; SH3 domain; Zinc.
FT CHAIN 1..270
FT /note="LIM zinc-binding domain-containing Nebulette"
FT /id="PRO_0000417585"
FT DOMAIN 3..63
FT /note="LIM zinc-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REPEAT 61..95
FT /note="Nebulin 1"
FT REPEAT 97..131
FT /note="Nebulin 2"
FT REPEAT 133..159
FT /note="Nebulin 3"
FT DOMAIN 210..270
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT MOD_RES 96
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 132
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 135
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 230
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 270 AA; 31113 MW; 1CC9E10C04E49E4D CRC64;
MNPQCARCGK VVYPTEKVNC LDKYWHKGCF HCEVCKMALN MNNYKGYEKK PYCNAHYPKQ
SFTTVADTPE NLRLKQQSEL QSQVKYKRDF EESKGRGFSI VTDTPELQRL KRTQEQISNV
KYHEDFEKTK GRGFTPVVDD PVTERVRKST QVVSDAAYKG VQPHVVEMDR RPGIIVAPVL
PGAYQQSHSQ GYGYMHQTSV SSMRSMQPPA HLRTYRAMYD YSAQDEDEVS FRDGDYIVNV
QPIDDGWMYG TVQRTGRTGM LPANYIEFVN
