LNK1_ARATH
ID LNK1_ARATH Reviewed; 616 AA.
AC A8MQN2; B9DGM4; Q9FMI3;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Protein LNK1 {ECO:0000303|PubMed:23818596};
DE AltName: Full=Night light-inducible and clock-regulated 1 {ECO:0000303|PubMed:23818596};
GN Name=LNK1 {ECO:0000303|PubMed:23818596};
GN OrderedLocusNames=At5g64170 {ECO:0000312|Araport:AT5G64170};
GN ORFNames=MHJ24.1 {ECO:0000312|EMBL:BAB10285.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, SUBCELLULAR LOCATION, GENE
RP FAMILY, AND NOMENCLATURE.
RX PubMed=23818596; DOI=10.1073/pnas.1302170110;
RA Rugnone M.L., Faigon Soverna A., Sanchez S.E., Schlaen R.G., Hernando C.E.,
RA Seymour D.K., Mancini E., Chernomoretz A., Weigel D., Mas P.,
RA Yanovsky M.J.;
RT "LNK genes integrate light and clock signaling networks at the core of the
RT Arabidopsis oscillator.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:12120-12125(2013).
RN [6]
RP INDUCTION BY LIGHT.
RX PubMed=25332490; DOI=10.1093/pcp/pcu144;
RA Mizuno T., Kitayama M., Oka H., Tsubouchi M., Takayama C., Nomoto Y.,
RA Yamashino T.;
RT "The EC night-time repressor plays a crucial role in modulating circadian
RT clock transcriptional circuitry by conservatively double-checking both
RT warm-night and night-time-light signals in a synergistic manner in
RT Arabidopsis thaliana.";
RL Plant Cell Physiol. 55:2139-2151(2014).
RN [7]
RP INDUCTION BY HEAT.
RX PubMed=24690904; DOI=10.4161/psb.28505;
RA Mizuno T., Takeuchi A., Nomoto Y., Nakamichi N., Yamashino T.;
RT "The LNK1 night light-inducible and clock-regulated gene is induced also in
RT response to warm-night through the circadian clock nighttime repressor in
RT Arabidopsis thaliana.";
RL Plant Signal. Behav. 9:E28505-E28505(2014).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE
RP SPECIFICITY, INTERACTION WITH CCA1; LHY; REV4 AND REV8, AND MUTAGENESIS OF
RP 503-ARG-ASP-504 AND 553-ASP-ARG-554.
RX PubMed=25012192; DOI=10.1105/tpc.114.126573;
RA Xie Q., Wang P., Liu X., Yuan L., Wang L., Zhang C., Li Y., Xing H.,
RA Zhi L., Yue Z., Zhao C., McClung C.R., Xu X.;
RT "LNK1 and LNK2 are transcriptional coactivators in the Arabidopsis
RT circadian oscillator.";
RL Plant Cell 26:2843-2857(2014).
RN [9]
RP FUNCTION.
RX PubMed=25848708; DOI=10.1080/15592324.2015.1010888;
RA Xing H., Wang P., Cui X., Zhang C., Wang L., Liu X., Yuan L., Li Y.,
RA Xie Q., Xu X.;
RT "LNK1 and LNK2 recruitment to the evening element require morning expressed
RT circadian related MYB-like transcription factors.";
RL Plant Signal. Behav. 10:E1010888-E1010888(2015).
RN [10]
RP FUNCTION, AND INTERACTION WITH RVE8.
RX PubMed=25848001; DOI=10.1073/pnas.1420792112;
RA Perez-Garcia P., Ma Y., Yanovsky M.J., Mas P.;
RT "Time-dependent sequestration of RVE8 by LNK proteins shapes the diurnal
RT oscillation of anthocyanin biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:5249-5253(2015).
CC -!- FUNCTION: Transcriptional coactivator necessary for expression of the
CC clock genes PRR5 and TOC1 (PubMed:25012192, PubMed:25848708).
CC Antagonizes REV8 function in the regulation of anthocyanin accumulation
CC (PubMed:25848001). Involved in red light input to the clock
CC (PubMed:25012192). Activates clock-controlled genes with afternoon peak
CC (PubMed:23818596). Mediates light inhibition of hypocotyl elongation
CC (PubMed:23818596). {ECO:0000269|PubMed:23818596,
CC ECO:0000269|PubMed:25012192, ECO:0000269|PubMed:25848001,
CC ECO:0000269|PubMed:25848708}.
CC -!- SUBUNIT: Interacts with CCA1, LHY, REV4 and REV8, but not with PRR7 or
CC PRR9. {ECO:0000269|PubMed:25012192, ECO:0000269|PubMed:25848001}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25012192}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A8MQN2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A8MQN2-2; Sequence=VSP_058215;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, seedlings,
CC cotyledons, inflorescences and siliques. Highest expression in root
CC tips, young leaves and vasculatur tissues.
CC {ECO:0000269|PubMed:25012192}.
CC -!- INDUCTION: Circadian-regulation (PubMed:23818596, PubMed:25012192).
CC Maximum levels of expression in the subjective morning
CC (PubMed:23818596, PubMed:25012192). Up-regulated by a light pulse in
CC the middle of the night via the phytochrome family of red/far-red light
CC photoreceptors (PubMed:23818596, PubMed:25332490, PubMed:25012192). Up-
CC regulated following a temperature upshift during the dark period
CC (PubMed:24690904). Repressed by members of the TOC1/PRR1 family of
CC clock genes (PubMed:23818596). {ECO:0000269|PubMed:23818596,
CC ECO:0000269|PubMed:24690904, ECO:0000269|PubMed:25012192,
CC ECO:0000269|PubMed:25332490}.
CC -!- DISRUPTION PHENOTYPE: No differences in hypocotyl length when grown in
CC complete darkness, but longer hypocotyls in plants under continuous
CC white or red light (PubMed:23818596). Lengthened circadian cycle
CC (PubMed:25012192). {ECO:0000269|PubMed:23818596,
CC ECO:0000269|PubMed:25012192}.
CC -!- MISCELLANEOUS: Unable to bind to DNA, but recruited to the evening
CC element (EE)-containing region of the PRR5 and TOC1 promoters through
CC its interaction with the DNA binding proteins REV8 and REV4
CC (PubMed:25012192, PubMed:25848708). {ECO:0000269|PubMed:25012192,
CC ECO:0000269|PubMed:25848708}.
CC -!- MISCELLANEOUS: The evening complex (EC) nighttime repressor is
CC implicated in the temperature responsiveness by binding to the LNK1
CC promoter (PubMed:25332490, PubMed:24690904). Responds also to some
CC extent to a dark/light transition in an EC-independent manner
CC (PubMed:25332490). {ECO:0000269|PubMed:24690904,
CC ECO:0000269|PubMed:25332490}.
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DR EMBL; AB008266; BAB10285.1; -; Genomic_DNA.
DR EMBL; AB008268; BAB10285.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED97849.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97850.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68756.1; -; Genomic_DNA.
DR EMBL; BT030360; ABO38773.1; -; mRNA.
DR EMBL; AK317207; BAH19891.1; -; mRNA.
DR RefSeq; NP_001078793.1; NM_001085324.2. [A8MQN2-1]
DR RefSeq; NP_001330478.1; NM_001345619.1. [A8MQN2-1]
DR RefSeq; NP_201222.1; NM_125813.5. [A8MQN2-2]
DR AlphaFoldDB; A8MQN2; -.
DR STRING; 3702.AT5G64170.2; -.
DR iPTMnet; A8MQN2; -.
DR PaxDb; A8MQN2; -.
DR PRIDE; A8MQN2; -.
DR ProteomicsDB; 238474; -. [A8MQN2-1]
DR EnsemblPlants; AT5G64170.1; AT5G64170.1; AT5G64170. [A8MQN2-2]
DR EnsemblPlants; AT5G64170.2; AT5G64170.2; AT5G64170. [A8MQN2-1]
DR EnsemblPlants; AT5G64170.3; AT5G64170.3; AT5G64170. [A8MQN2-1]
DR GeneID; 836538; -.
DR Gramene; AT5G64170.1; AT5G64170.1; AT5G64170. [A8MQN2-2]
DR Gramene; AT5G64170.2; AT5G64170.2; AT5G64170. [A8MQN2-1]
DR Gramene; AT5G64170.3; AT5G64170.3; AT5G64170. [A8MQN2-1]
DR KEGG; ath:AT5G64170; -.
DR Araport; AT5G64170; -.
DR TAIR; locus:2164416; AT5G64170.
DR eggNOG; ENOG502QY4B; Eukaryota.
DR InParanoid; A8MQN2; -.
DR OMA; PSMIHGS; -.
DR OrthoDB; 1370250at2759; -.
DR PhylomeDB; A8MQN2; -.
DR PRO; PR:A8MQN2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; A8MQN2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0070063; F:RNA polymerase binding; IDA:TAIR.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:TAIR.
DR GO; GO:0032922; P:circadian regulation of gene expression; IDA:TAIR.
DR GO; GO:0006354; P:DNA-templated transcription, elongation; IDA:TAIR.
DR GO; GO:0009649; P:entrainment of circadian clock; IGI:TAIR.
DR InterPro; IPR039928; LNK.
DR PANTHER; PTHR33334; PTHR33334; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Biological rhythms; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..616
FT /note="Protein LNK1"
FT /id="PRO_0000436031"
FT REGION 72..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 361..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 410..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 567..616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..395
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 567..595
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..50
FT /note="Missing (in isoform 2)"
FT /id="VSP_058215"
FT MUTAGEN 503..504
FT /note="RD->GG: Reduced interaction with REV4."
FT /evidence="ECO:0000269|PubMed:25012192"
FT MUTAGEN 553..554
FT /note="DR->GG: Reduced interaction with REV4."
FT /evidence="ECO:0000269|PubMed:25012192"
FT CONFLICT 284
FT /note="L -> F (in Ref. 4; BAH19891)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 616 AA; 68181 MW; 051872E2FB836F8F CRC64;
MSDLYIHELG DYLSDEFHGN DDGIVPDSAY EDGGQFPILV SNRKKRRNDD MGSGTNHLKS
NTFIKREANM LGKNPWPEKD SGGSSVSRDT GTGKDVQDMT LEDTNTSDHG FNGGHVDVVE
NFSTGDPMLC DTSAATNDGV YNYSLNSIPD AENDLSFFDN GDKEKNDLFY GWGDIGNFED
VDNMLRSCDS TFGLDSLNNE GDLGWFSSAQ PNEETAGAMT DDLKPDKMLE NQRTAMLQVE
DFLNNSEPNH AVEDEYGYTI EDDSAQGKSS QNVFDTSLQK KDILMLDVEA NLEKKQTDHL
HHLDGKSDGF SENSFTLQHS GISREIMDTN QYYPPSAFQQ RDVPYSHFNC EQPSVQVSAC
ESKSGIKSEN KPSPSSASNE SYTSNHAQSI ESLQGPTVDD RFRKVFETRA NLLPGQDMPP
SFAANTKKSS KTDSMVFPDA APIQKIGLEN DHRKAATELE TSNMQGSSCV SSVVDDISLE
ATSFRQLQQV IEQLDVRTKL CIRDSLYRLA KSAEQRHHGG NRPEKGAGSH LVTGEADKYA
GFMDIETDTN PIDRSIAHLL FHRPSDSSLS SDNNVLSYKS HPMIPQPNSS PSLRIEKQEE
TTELRPEAEV VTSDNN
