LNK2_ARATH
ID LNK2_ARATH Reviewed; 663 AA.
AC F4JCX9; A0A1I9LQ06; A0A1I9LQ07; F4JCX8; F4JCY0; F4JCY1; Q9M1H7;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Protein LNK2 {ECO:0000303|PubMed:23818596};
DE AltName: Full=Night light-inducible and clock-regulated 2 {ECO:0000303|PubMed:23818596};
GN Name=LNK2 {ECO:0000303|PubMed:23818596};
GN OrderedLocusNames=At3g54500 {ECO:0000312|Araport:AT3G54500};
GN ORFNames=T14E10.70 {ECO:0000312|EMBL:CAB77570.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23818596; DOI=10.1073/pnas.1302170110;
RA Rugnone M.L., Faigon Soverna A., Sanchez S.E., Schlaen R.G., Hernando C.E.,
RA Seymour D.K., Mancini E., Chernomoretz A., Weigel D., Mas P.,
RA Yanovsky M.J.;
RT "LNK genes integrate light and clock signaling networks at the core of the
RT Arabidopsis oscillator.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:12120-12125(2013).
RN [4]
RP INDUCTION BY HEAT.
RX PubMed=24690904; DOI=10.4161/psb.28505;
RA Mizuno T., Takeuchi A., Nomoto Y., Nakamichi N., Yamashino T.;
RT "The LNK1 night light-inducible and clock-regulated gene is induced also in
RT response to warm-night through the circadian clock nighttime repressor in
RT Arabidopsis thaliana.";
RL Plant Signal. Behav. 9:E28505-E28505(2014).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE
RP SPECIFICITY, AND INTERACTION WITH CCA1; LHY; REV4 AND REV8.
RX PubMed=25012192; DOI=10.1105/tpc.114.126573;
RA Xie Q., Wang P., Liu X., Yuan L., Wang L., Zhang C., Li Y., Xing H.,
RA Zhi L., Yue Z., Zhao C., McClung C.R., Xu X.;
RT "LNK1 and LNK2 are transcriptional coactivators in the Arabidopsis
RT circadian oscillator.";
RL Plant Cell 26:2843-2857(2014).
RN [6]
RP FUNCTION.
RX PubMed=25848708; DOI=10.1080/15592324.2015.1010888;
RA Xing H., Wang P., Cui X., Zhang C., Wang L., Liu X., Yuan L., Li Y.,
RA Xie Q., Xu X.;
RT "LNK1 and LNK2 recruitment to the evening element require morning expressed
RT circadian related MYB-like transcription factors.";
RL Plant Signal. Behav. 10:E1010888-E1010888(2015).
RN [7]
RP FUNCTION, AND INTERACTION WITH RVE8.
RX PubMed=25848001; DOI=10.1073/pnas.1420792112;
RA Perez-Garcia P., Ma Y., Yanovsky M.J., Mas P.;
RT "Time-dependent sequestration of RVE8 by LNK proteins shapes the diurnal
RT oscillation of anthocyanin biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:5249-5253(2015).
CC -!- FUNCTION: Transcriptional coactivator necessary for expression of the
CC clock genes PRR5 and TOC1 (PubMed:25012192, PubMed:25848708).
CC Antagonizes REV8 function in the regulation of anthocyanin accumulation
CC (PubMed:25848001). Involved in red light input to the clock
CC (PubMed:25012192). Activates clock-controlled genes with afternoon peak
CC (PubMed:23818596). Mediates light inhibition of hypocotyl elongation
CC (PubMed:23818596). Unable to bind to DNA, but recruited to the evening
CC element (EE)-containing region of the PRR5 and TOC1 promoters through
CC its interaction with the DNA binding proteins REV8 and REV4
CC (PubMed:25012192, PubMed:25848708). {ECO:0000269|PubMed:23818596,
CC ECO:0000269|PubMed:25012192, ECO:0000269|PubMed:25848001,
CC ECO:0000269|PubMed:25848708}.
CC -!- SUBUNIT: Interacts with CCA1, LHY, REV4 and REV8, but not with PRR7 or
CC PRR9. {ECO:0000269|PubMed:25012192, ECO:0000269|PubMed:25848001}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25012192}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=F4JCX9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4JCX9-2; Sequence=VSP_058216, VSP_058219, VSP_058220;
CC Name=3;
CC IsoId=F4JCX9-3; Sequence=VSP_058218, VSP_058220;
CC Name=4;
CC IsoId=F4JCX9-4; Sequence=VSP_058217, VSP_058220;
CC Name=5;
CC IsoId=F4JCX9-5; Sequence=VSP_058219, VSP_058220;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, seedlings,
CC cotyledons, inflorescences and siliques. Highest expression in root
CC tips, young leaves and vasculatur tissues.
CC {ECO:0000269|PubMed:25012192}.
CC -!- INDUCTION: Circadian-regulation (PubMed:23818596, PubMed:25012192).
CC Maximum levels of expression in the subjective morning
CC (PubMed:23818596, PubMed:25012192). Up-regulated by a light pulse in
CC the middle of the night via the phytochrome family of red/far-red light
CC photoreceptors (PubMed:23818596, PubMed:25012192). Up-regulated
CC following a temperature upshift during the dark period
CC (PubMed:24690904). Repressed by members of the TOC1/PRR1 family of
CC clock genes (PubMed:23818596). {ECO:0000269|PubMed:23818596,
CC ECO:0000269|PubMed:24690904, ECO:0000269|PubMed:25012192}.
CC -!- DISRUPTION PHENOTYPE: No differences in hypocotyl length when grown in
CC complete darkness, but longer hypocotyls in plants under continuous
CC white or red light (PubMed:23818596). Lengthened circadian cycle
CC (PubMed:25012192). {ECO:0000269|PubMed:23818596,
CC ECO:0000269|PubMed:25012192}.
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DR EMBL; AL138656; CAB77570.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79239.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79242.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64663.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64664.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64665.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64666.1; -; Genomic_DNA.
DR PIR; T47609; T47609.
DR RefSeq; NP_001190089.1; NM_001203160.1. [F4JCX9-1]
DR RefSeq; NP_001326676.1; NM_001339675.1. [F4JCX9-2]
DR RefSeq; NP_001326677.1; NM_001339676.1. [F4JCX9-5]
DR RefSeq; NP_001326678.1; NM_001339674.1. [F4JCX9-5]
DR RefSeq; NP_191014.2; NM_115307.5. [F4JCX9-3]
DR RefSeq; NP_974432.2; NM_202703.3. [F4JCX9-2]
DR AlphaFoldDB; F4JCX9; -.
DR STRING; 3702.AT3G54500.3; -.
DR PRIDE; F4JCX9; -.
DR ProteomicsDB; 238475; -. [F4JCX9-1]
DR EnsemblPlants; AT3G54500.1; AT3G54500.1; AT3G54500. [F4JCX9-3]
DR EnsemblPlants; AT3G54500.4; AT3G54500.4; AT3G54500. [F4JCX9-1]
DR EnsemblPlants; AT3G54500.5; AT3G54500.5; AT3G54500. [F4JCX9-5]
DR EnsemblPlants; AT3G54500.6; AT3G54500.6; AT3G54500. [F4JCX9-2]
DR EnsemblPlants; AT3G54500.7; AT3G54500.7; AT3G54500. [F4JCX9-5]
DR EnsemblPlants; AT3G54500.8; AT3G54500.8; AT3G54500. [F4JCX9-2]
DR GeneID; 824615; -.
DR Gramene; AT3G54500.1; AT3G54500.1; AT3G54500. [F4JCX9-3]
DR Gramene; AT3G54500.4; AT3G54500.4; AT3G54500. [F4JCX9-1]
DR Gramene; AT3G54500.5; AT3G54500.5; AT3G54500. [F4JCX9-5]
DR Gramene; AT3G54500.6; AT3G54500.6; AT3G54500. [F4JCX9-2]
DR Gramene; AT3G54500.7; AT3G54500.7; AT3G54500. [F4JCX9-5]
DR Gramene; AT3G54500.8; AT3G54500.8; AT3G54500. [F4JCX9-2]
DR KEGG; ath:AT3G54500; -.
DR Araport; AT3G54500; -.
DR TAIR; locus:2096981; AT3G54500.
DR eggNOG; ENOG502QS7P; Eukaryota.
DR OMA; NNEYSGK; -.
DR PRO; PR:F4JCX9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JCX9; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:TAIR.
DR GO; GO:0032922; P:circadian regulation of gene expression; IDA:TAIR.
DR GO; GO:0009649; P:entrainment of circadian clock; IGI:TAIR.
DR InterPro; IPR039928; LNK.
DR PANTHER; PTHR33334; PTHR33334; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Biological rhythms; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..663
FT /note="Protein LNK2"
FT /id="PRO_0000436032"
FT REGION 528..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 590..663
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..628
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..653
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..80
FT /note="Missing (in isoform 2)"
FT /id="VSP_058216"
FT VAR_SEQ 1..11
FT /note="Missing (in isoform 4)"
FT /id="VSP_058217"
FT VAR_SEQ 119..150
FT /note="Missing (in isoform 3)"
FT /id="VSP_058218"
FT VAR_SEQ 120..150
FT /note="Missing (in isoform 2 and isoform 5)"
FT /id="VSP_058219"
FT VAR_SEQ 195..222
FT /note="DGFAAMMSLYLVMAVSAVVMSYGHLLKI -> NDVPIFGDGSLSGGDELWSS
FT SKDVSNSPKSLSSMLDSQDLGLDIR (in isoform 2, isoform 3, isoform
FT 4 and isoform 5)"
FT /id="VSP_058220"
SQ SEQUENCE 663 AA; 73593 MW; 23D5CFC6793C5518 CRC64;
MFDWEEEELT NMIWGDDAET GDHIVPFKVR SEQLNKKEQI EESKTAEQKI TGTKIDLHDK
NLGSSSSHNV DEGLPQPDFC MSSWPDTSLT NATKVDQDLS ATELSKCLAE PVRYDSTRGG
AFLLKQSCFT WVRSFQSNHF KSCVLTLFLP EKTSELGKGP DIFHSSDESK EQGDFDDYSW
ANIGSFDDLD RMFSDGFAAM MSLYLVMAVS AVVMSYGHLL KITEFEQQEN QQFPLTGKAN
GLSSQSVPSV RVTLKADQYR EHKGQPSVED QPYQQNKMMK FSKMPGTSEA RPFQELYGQR
IPFSNSAGKC VNQLAPPQSS LMAVNLLSES EGSGTSHYSH MPNQYMANSA FGNLANPYSS
VPVISAVQHP DVRNQLMHPS YNPATATSVN MATDASARPS TMTPQEKLEK LRRRQQMQAM
LAIQRQQQQF SHQVPVADQS ITQNCLQDIP LQLVDKTNLQ GLTAMPSFDP SSSLEQDDSG
KFAAAVDNSA EFAVLYRLQD VVAKLDMGTR TCIRDSLFRL AGSAAQRHYT SDTSHSNKTS
QDDQEVIPRE ESRYRYAGMP DTEAVTNPTD RTVAHLLFHR PFDMLAAKRM EGPESPASSK
MGTEEKGNFP KCSIRETHLT KQKAQKEEGP ADSLALGNAP NSGSSSTVGE RVVEASQGNK
RKL
