LNRK_BACSU
ID LNRK_BACSU Reviewed; 220 AA.
AC P94439; Q796Z8;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Transcriptional regulatory protein LnrK {ECO:0000305};
GN Name=lnrK {ECO:0000303|PubMed:28461449};
GN Synonyms=linK {ECO:0000312|EMBL:CAB12659.1}, yfiK;
GN OrderedLocusNames=BSU08300;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / AC327;
RX PubMed=8973323; DOI=10.1016/s0378-1119(96)00495-7;
RA Yamamoto H., Uchiyama S., Sekiguchi J.;
RT "The Bacillus subtilis chromosome region near 78 degrees contains the genes
RT encoding a new two-component system, three ABC transporters and a lipase.";
RL Gene 181:147-151(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ASP-54.
RX PubMed=26647299; DOI=10.1371/journal.pgen.1005722;
RA Stubbendieck R.M., Straight P.D.;
RT "Escape from lethal bacterial competition through coupled activation of
RT antibiotic resistance and a mobilized subpopulation.";
RL PLoS Genet. 11:E1005722-E1005722(2015).
RN [5]
RP FUNCTION.
RX PubMed=28461449; DOI=10.1128/jb.00186-17;
RA Stubbendieck R.M., Straight P.D.;
RT "Linearmycins activate a two-component signaling system involved in
RT bacterial competition and biofilm morphology.";
RL J. Bacteriol. 199:E00186-E00186(2017).
CC -!- FUNCTION: Required for resistance to linearmycins, a family of
CC antibiotic-specialized metabolites produced by some streptomycetes
CC (PubMed:26647299, PubMed:28461449). Member of the two-component
CC regulatory system LnrJ/LnrK, which induces expression of the LnrLMN ABC
CC transporter in response to linearmycins and other polyenes
CC (PubMed:11717295, PubMed:26647299, PubMed:28461449). Probably binds to
CC the promoter region of the lnrLMN operon and directly regulates its
CC expression (Probable). May also promote biofilm formation
CC (PubMed:28461449). {ECO:0000269|PubMed:11717295,
CC ECO:0000269|PubMed:26647299, ECO:0000269|PubMed:28461449,
CC ECO:0000305|PubMed:28461449}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by LnrJ. {ECO:0000305|PubMed:28461449}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant does not show any observable
CC phenotype. {ECO:0000269|PubMed:26647299}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D78508; BAA11401.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12659.1; -; Genomic_DNA.
DR PIR; JC5359; JC5359.
DR RefSeq; NP_388711.1; NC_000964.3.
DR RefSeq; WP_003242530.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; P94439; -.
DR SMR; P94439; -.
DR STRING; 224308.BSU08300; -.
DR PaxDb; P94439; -.
DR PRIDE; P94439; -.
DR EnsemblBacteria; CAB12659; CAB12659; BSU_08300.
DR GeneID; 939209; -.
DR KEGG; bsu:BSU08300; -.
DR PATRIC; fig|224308.179.peg.896; -.
DR eggNOG; COG2197; Bacteria.
DR InParanoid; P94439; -.
DR OMA; DDPMMQL; -.
DR PhylomeDB; P94439; -.
DR BioCyc; BSUB:BSU08300-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..220
FT /note="Transcriptional regulatory protein LnrK"
FT /id="PRO_0000360775"
FT DOMAIN 3..119
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 151..216
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 175..194
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MUTAGEN 54
FT /note="D->A: Mutant is sensitive to linearmycins."
FT /evidence="ECO:0000269|PubMed:26647299"
SQ SEQUENCE 220 AA; 24371 MW; FD108A9223662561 CRC64;
MIKIIITDDQ DIVREGLASL LQLREELDVI ATARNGQEAF EKAKELEPDI VLMDIRMPVS
NGVEGTKLIT SSLPSVKVLM LTTFKDSALI AEALEEGASG YLLKDMSADT IVKAVMTVHS
GGMVLPPELT AQMLNEWKRE KQLKGINEIE KPNELLDLTE REIEVLAELG YGLNNKEIAE
KLYITEGTVK NHVSNIISKL AVRDRTQAAI YSVRYGVSVF
