ID   LNRL_BACSU              Reviewed;         311 AA.
AC   P94440; Q796Z7;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Linearmycin resistance ATP-binding protein LnrL {ECO:0000305};
DE            EC=7.6.2.- {ECO:0000305};
GN   Name=lnrL {ECO:0000303|PubMed:28461449};
GN   Synonyms=bifL {ECO:0000312|EMBL:CAB12660.2}, yfiL;
GN   OrderedLocusNames=BSU08310;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / AC327;
RX   PubMed=8973323; DOI=10.1016/s0378-1119(96)00495-7;
RA   Yamamoto H., Uchiyama S., Sekiguchi J.;
RT   "The Bacillus subtilis chromosome region near 78 degrees contains the genes
RT   encoding a new two-component system, three ABC transporters and a lipase.";
RL   Gene 181:147-151(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 107.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   FUNCTION.
RX   PubMed=26647299; DOI=10.1371/journal.pgen.1005722;
RA   Stubbendieck R.M., Straight P.D.;
RT   "Escape from lethal bacterial competition through coupled activation of
RT   antibiotic resistance and a mobilized subpopulation.";
RL   PLoS Genet. 11:E1005722-E1005722(2015).
RN   [5]
RP   FUNCTION, SUBUNIT, AND INDUCTION.
RX   PubMed=28461449; DOI=10.1128/jb.00186-17;
RA   Stubbendieck R.M., Straight P.D.;
RT   "Linearmycins activate a two-component signaling system involved in
RT   bacterial competition and biofilm morphology.";
RL   J. Bacteriol. 199:E00186-E00186(2017).
CC   -!- FUNCTION: Required for resistance to linearmycins, a family of
CC       antibiotic-specialized metabolites produced by some streptomycetes
CC       (PubMed:26647299, PubMed:28461449). Part of the ABC transporter complex
CC       LnrLMN that probably facilitates linearmycin removal from the membrane.
CC       Responsible for energy coupling to the transport system
CC       (PubMed:28461449). Also mediates KinC-dependent biofilm morphology
CC       (PubMed:28461449). {ECO:0000269|PubMed:26647299,
CC       ECO:0000269|PubMed:28461449}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LnrL) and
CC       two transmembrane proteins (LnrM and LnrN).
CC       {ECO:0000305|PubMed:28461449}.
CC   -!- INDUCTION: Induced in response to linearmycins and other polyenes via
CC       the two-component regulatory system LnrJ/LnrK.
CC       {ECO:0000269|PubMed:28461449}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; D78508; BAA11402.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12660.2; -; Genomic_DNA.
DR   PIR; G69803; G69803.
DR   RefSeq; NP_388712.2; NC_000964.3.
DR   RefSeq; WP_003244346.1; NZ_JNCM01000032.1.
DR   AlphaFoldDB; P94440; -.
DR   SMR; P94440; -.
DR   STRING; 224308.BSU08310; -.
DR   TCDB; 3.A.1.105.19; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P94440; -.
DR   PRIDE; P94440; -.
DR   EnsemblBacteria; CAB12660; CAB12660; BSU_08310.
DR   GeneID; 936175; -.
DR   KEGG; bsu:BSU08310; -.
DR   PATRIC; fig|224308.179.peg.898; -.
DR   eggNOG; COG1131; Bacteria.
DR   InParanoid; P94440; -.
DR   OMA; AHENLMF; -.
DR   PhylomeDB; P94440; -.
DR   BioCyc; BSUB:BSU08310-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Translocase;
KW   Transport.
FT   CHAIN           1..311
FT                   /note="Linearmycin resistance ATP-binding protein LnrL"
FT                   /id="PRO_0000360194"
FT   DOMAIN          2..232
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         34..41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   CONFLICT        107
FT                   /note="G -> D (in Ref. 1; BAA11402)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   311 AA;  34050 MW;  089748F7E7CE5E8D CRC64;
     MLQAENIKKA YGKKTIVKGI SFSLKKGESF GLLGPNGAGK STTISMISGL VPHDSGNITV
     GGYVIGKETA KAKQKIGIVP QEIALYPTLT AHENLMFWGK MYGLTHGEAK KRAAEVLEYV
     GLTERAKDKI ETFSGGMKRR INIGAALMHK PELLIMDEPT VGIDPQSRNH ILETVKQLNE
     TGMTVIYTSH YMEEVEFLCD RIGIIDQGEM IAIGTKTDLC SRLGGDTIIQ LTVSGINEAF
     LVAIRSLAHV NDVTVHELEL KIDISAAHHE KVVTSLLAEA TAHHINLLSL QVQEPNLERL
     FLNLTGRTLR D