LNX2_HUMAN
ID LNX2_HUMAN Reviewed; 690 AA.
AC Q8N448; Q5W0P0; Q6ZMH2; Q96SH4;
DT 28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Ligand of Numb protein X 2;
DE AltName: Full=Numb-binding protein 2;
DE AltName: Full=PDZ domain-containing RING finger protein 1;
GN Name=LNX2; Synonyms=PDZRN1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Melanoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 522-690.
RC TISSUE=Colon;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 336-424.
RG Structural genomics consortium (SGC);
RT "Crystal structure of the second Pdz domain of the human Numb-binding
RT protein 2.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- SUBUNIT: Interacts with the phosphotyrosine interaction domain of NUMB.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q8N448; P06576: ATP5F1B; NbExp=4; IntAct=EBI-2340947, EBI-356231;
CC Q8N448; P54253: ATXN1; NbExp=6; IntAct=EBI-2340947, EBI-930964;
CC Q8N448; P45973: CBX5; NbExp=3; IntAct=EBI-2340947, EBI-78219;
CC Q8N448; Q13643: FHL3; NbExp=4; IntAct=EBI-2340947, EBI-741101;
CC Q8N448; P04792: HSPB1; NbExp=3; IntAct=EBI-2340947, EBI-352682;
CC Q8N448; P42858: HTT; NbExp=3; IntAct=EBI-2340947, EBI-466029;
CC Q8N448; Q9UHH9: IP6K2; NbExp=3; IntAct=EBI-2340947, EBI-747509;
CC Q8N448; O60333-2: KIF1B; NbExp=3; IntAct=EBI-2340947, EBI-10975473;
CC Q8N448; Q13163: MAP2K5; NbExp=3; IntAct=EBI-2340947, EBI-307294;
CC Q8N448; P45984: MAPK9; NbExp=3; IntAct=EBI-2340947, EBI-713568;
CC Q8N448; Q96HT8: MRFAP1L1; NbExp=4; IntAct=EBI-2340947, EBI-748896;
CC Q8N448; Q8WY64: MYLIP; NbExp=4; IntAct=EBI-2340947, EBI-6952711;
CC Q8N448; Q96CV9: OPTN; NbExp=3; IntAct=EBI-2340947, EBI-748974;
CC Q8N448; Q9H8K7: PAAT; NbExp=3; IntAct=EBI-2340947, EBI-714785;
CC Q8N448; Q86TG7: PEG10; NbExp=3; IntAct=EBI-2340947, EBI-2858265;
CC Q8N448; Q8IUZ5: PHYKPL; NbExp=3; IntAct=EBI-2340947, EBI-751947;
CC Q8N448; Q96EY7: PTCD3; NbExp=3; IntAct=EBI-2340947, EBI-721110;
CC Q8N448; Q96I15: SCLY; NbExp=3; IntAct=EBI-2340947, EBI-2823066;
CC Q8N448; P37840: SNCA; NbExp=3; IntAct=EBI-2340947, EBI-985879;
CC Q8N448; P00441: SOD1; NbExp=3; IntAct=EBI-2340947, EBI-990792;
CC Q8N448; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-2340947, EBI-5235340;
CC Q8N448; Q13148: TARDBP; NbExp=6; IntAct=EBI-2340947, EBI-372899;
CC Q8N448; P37173: TGFBR2; NbExp=3; IntAct=EBI-2340947, EBI-296151;
CC Q8N448; O76024: WFS1; NbExp=3; IntAct=EBI-2340947, EBI-720609;
CC Q8N448; K9N5R3: E; Xeno; NbExp=2; IntAct=EBI-2340947, EBI-26374535;
CC Q8N448; P09022: Hoxa1; Xeno; NbExp=3; IntAct=EBI-2340947, EBI-3957603;
CC Q8N448; P03410: tax; Xeno; NbExp=4; IntAct=EBI-2340947, EBI-9676218;
CC Q8N448; P14079: tax; Xeno; NbExp=4; IntAct=EBI-2340947, EBI-9675698;
CC Q8N448; P0C206; Xeno; NbExp=4; IntAct=EBI-2340947, EBI-9675596;
CC Q8N448; Q85601; Xeno; NbExp=4; IntAct=EBI-2340947, EBI-9676175;
CC -!- DOMAIN: The NPXY motif is required for the interaction with the PID
CC domain of NUMB. It is however not sufficient.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD18754.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL138699; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC036755; AAH36755.1; -; mRNA.
DR EMBL; AK172771; BAD18754.1; ALT_INIT; mRNA.
DR CCDS; CCDS9323.1; -.
DR RefSeq; NP_699202.1; NM_153371.3.
DR RefSeq; XP_016875923.1; XM_017020434.1.
DR PDB; 2VWR; X-ray; 1.30 A; A=336-424.
DR PDB; 5DIN; X-ray; 1.86 A; A/B=20-147.
DR PDB; 5E11; X-ray; 1.80 A; A=336-424.
DR PDB; 5E1Y; X-ray; 1.01 A; A=336-424.
DR PDB; 5E21; X-ray; 1.01 A; A=336-424.
DR PDB; 5E22; X-ray; 1.80 A; A/B=336-424.
DR PDBsum; 2VWR; -.
DR PDBsum; 5DIN; -.
DR PDBsum; 5E11; -.
DR PDBsum; 5E1Y; -.
DR PDBsum; 5E21; -.
DR PDBsum; 5E22; -.
DR AlphaFoldDB; Q8N448; -.
DR SMR; Q8N448; -.
DR BioGRID; 128798; 107.
DR IntAct; Q8N448; 110.
DR MINT; Q8N448; -.
DR STRING; 9606.ENSP00000325929; -.
DR MoonDB; Q8N448; Predicted.
DR iPTMnet; Q8N448; -.
DR PhosphoSitePlus; Q8N448; -.
DR BioMuta; LNX2; -.
DR DMDM; 29840784; -.
DR EPD; Q8N448; -.
DR jPOST; Q8N448; -.
DR MassIVE; Q8N448; -.
DR MaxQB; Q8N448; -.
DR PaxDb; Q8N448; -.
DR PeptideAtlas; Q8N448; -.
DR PRIDE; Q8N448; -.
DR ProteomicsDB; 71884; -.
DR Antibodypedia; 22674; 177 antibodies from 23 providers.
DR DNASU; 222484; -.
DR Ensembl; ENST00000316334.5; ENSP00000325929.3; ENSG00000139517.9.
DR Ensembl; ENST00000649248.1; ENSP00000497224.1; ENSG00000139517.9.
DR GeneID; 222484; -.
DR KEGG; hsa:222484; -.
DR MANE-Select; ENST00000316334.5; ENSP00000325929.3; NM_153371.4; NP_699202.1.
DR UCSC; uc001url.5; human.
DR CTD; 222484; -.
DR DisGeNET; 222484; -.
DR GeneCards; LNX2; -.
DR HGNC; HGNC:20421; LNX2.
DR HPA; ENSG00000139517; Low tissue specificity.
DR MIM; 609733; gene.
DR neXtProt; NX_Q8N448; -.
DR OpenTargets; ENSG00000139517; -.
DR PharmGKB; PA134899015; -.
DR VEuPathDB; HostDB:ENSG00000139517; -.
DR eggNOG; KOG3528; Eukaryota.
DR GeneTree; ENSGT00940000158381; -.
DR HOGENOM; CLU_021213_0_0_1; -.
DR InParanoid; Q8N448; -.
DR OMA; CLGTGTV; -.
DR OrthoDB; 615205at2759; -.
DR PhylomeDB; Q8N448; -.
DR TreeFam; TF330709; -.
DR PathwayCommons; Q8N448; -.
DR SignaLink; Q8N448; -.
DR BioGRID-ORCS; 222484; 16 hits in 1117 CRISPR screens.
DR ChiTaRS; LNX2; human.
DR EvolutionaryTrace; Q8N448; -.
DR GenomeRNAi; 222484; -.
DR Pharos; Q8N448; Tbio.
DR PRO; PR:Q8N448; -.
DR Proteomes; UP000005640; Chromosome 13.
DR RNAct; Q8N448; protein.
DR Bgee; ENSG00000139517; Expressed in jejunal mucosa and 189 other tissues.
DR Genevisible; Q8N448; HS.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030165; F:PDZ domain binding; IEA:Ensembl.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0061351; P:neural precursor cell proliferation; IEA:Ensembl.
DR GO; GO:0030182; P:neuron differentiation; IEA:Ensembl.
DR Gene3D; 2.30.42.10; -; 4.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF00595; PDZ; 4.
DR SMART; SM00228; PDZ; 4.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF50156; SSF50156; 4.
DR PROSITE; PS50106; PDZ; 4.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..690
FT /note="Ligand of Numb protein X 2"
FT /id="PRO_0000055915"
FT DOMAIN 233..318
FT /note="PDZ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 339..422
FT /note="PDZ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 468..554
FT /note="PDZ 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 600..688
FT /note="PDZ 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT ZN_FING 50..88
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 198..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..455
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 208..211
FT /note="NPXY motif"
FT COMPBIAS 430..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 198
FT /note="S -> P (in dbSNP:rs8002697)"
FT /id="VAR_024612"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:5DIN"
FT STRAND 39..42
FT /evidence="ECO:0007829|PDB:5DIN"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:5DIN"
FT TURN 51..53
FT /evidence="ECO:0007829|PDB:5DIN"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:5DIN"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:5DIN"
FT HELIX 71..77
FT /evidence="ECO:0007829|PDB:5DIN"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:5DIN"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:5DIN"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:5DIN"
FT HELIX 101..108
FT /evidence="ECO:0007829|PDB:5DIN"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:5DIN"
FT TURN 117..119
FT /evidence="ECO:0007829|PDB:5DIN"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:5DIN"
FT HELIX 126..128
FT /evidence="ECO:0007829|PDB:5DIN"
FT HELIX 129..135
FT /evidence="ECO:0007829|PDB:5DIN"
FT STRAND 336..343
FT /evidence="ECO:0007829|PDB:5E1Y"
FT STRAND 353..356
FT /evidence="ECO:0007829|PDB:5E1Y"
FT STRAND 359..361
FT /evidence="ECO:0007829|PDB:5E1Y"
FT STRAND 363..369
FT /evidence="ECO:0007829|PDB:5E1Y"
FT HELIX 374..378
FT /evidence="ECO:0007829|PDB:5E1Y"
FT STRAND 386..390
FT /evidence="ECO:0007829|PDB:5E1Y"
FT HELIX 400..408
FT /evidence="ECO:0007829|PDB:5E1Y"
FT STRAND 412..422
FT /evidence="ECO:0007829|PDB:5E1Y"
SQ SEQUENCE 690 AA; 76004 MW; A3F941A477721F1D CRC64;
MGTTSDEMVS VEQTSSSSLN PLCFECGQQH WTRENHLYNY QNEVDDDLVC HICLQPLLQP
LDTPCGHTFC YKCLRNFLQE KDFCPLDRKR LHFKLCKKSS ILVHKLLDKL LVLCPFSSVC
KDVMQRCDLE AHLKNRCPGA SHRRVALERR KTSRTQAEIE NENGPTLLDP AGTLSPEADC
LGTGAVPVER HLTSASLSTW SEEPGLDNPA FEESAGADTT QQPLSLPEGE ITTIEIHRSN
PYIQLGISIV GGNETPLINI VIQEVYRDGV IARDGRLLAG DQILQVNNYN ISNVSHNYAR
AVLSQPCNTL HLTVLRERRF GNRAHNHSDS NSPREEIFQV ALHKRDSGEQ LGIKLVRRTD
EPGVFILDLL EGGLAAQDGR LSSNDRVLAI NGHDLKYGTP ELAAQIIQAS GERVNLTIAR
PGKPQPGNTI REAGNHSSSS QHHTPPPYYS RPSSHKDLTQ CVTCQEKHIT VKKEPHESLG
MTVAGGRGSK SGELPIFVTS VPPHGCLARD GRIKRGDVLL NINGIDLTNL SHSEAVAMLK
ASAASPAVAL KALEVQIVEE ATQNAEEQPS TFSENEYDAS WSPSWVMWLG LPSTLHSCHD
IVLRRSYLGS WGFSIVGGYE ENHTNQPFFI KTIVLGTPAY YDGRLKCGDM IVAVNGLSTV
GMSHSALVPM LKEQRNKVTL TVICWPGSLV
