LNX2_MOUSE
ID LNX2_MOUSE Reviewed; 687 AA.
AC Q91XL2; A6PW04; Q8CBE1;
DT 28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Ligand of Numb protein X 2;
DE AltName: Full=Numb-binding protein 2;
GN Name=Lnx2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH NUMB, AND
RP MUTAGENESIS OF 207-ASN--PHE-210.
RC STRAIN=BALB/cJ; TISSUE=Cerebellum;
RX PubMed=11922143; DOI=10.1006/mcne.2001.1024;
RA Rice D.S., Northcutt G.M., Kurschner C.;
RT "The Lnx family proteins function as molecular scaffolds for Numb family
RT proteins.";
RL Mol. Cell. Neurosci. 18:525-540(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBUNIT: Interacts with the phosphotyrosine interaction domain of NUMB.
CC {ECO:0000269|PubMed:11922143}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:11922143}.
CC -!- DOMAIN: The NPXY motif is required for the interaction with the PID
CC domain of NUMB. It is however not sufficient.
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DR EMBL; AF401681; AAK94476.1; -; mRNA.
DR EMBL; AK036218; BAC29350.1; -; mRNA.
DR EMBL; AL513345; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466614; EDL05824.1; -; Genomic_DNA.
DR CCDS; CCDS19875.1; -.
DR RefSeq; NP_542985.4; NM_080795.4.
DR RefSeq; XP_006504863.1; XM_006504800.3.
DR AlphaFoldDB; Q91XL2; -.
DR SMR; Q91XL2; -.
DR BioGRID; 228328; 45.
DR CORUM; Q91XL2; -.
DR STRING; 10090.ENSMUSP00000016664; -.
DR iPTMnet; Q91XL2; -.
DR PhosphoSitePlus; Q91XL2; -.
DR EPD; Q91XL2; -.
DR MaxQB; Q91XL2; -.
DR PaxDb; Q91XL2; -.
DR PRIDE; Q91XL2; -.
DR ProteomicsDB; 286226; -.
DR Antibodypedia; 22674; 177 antibodies from 23 providers.
DR DNASU; 140887; -.
DR Ensembl; ENSMUST00000016664; ENSMUSP00000016664; ENSMUSG00000016520.
DR GeneID; 140887; -.
DR KEGG; mmu:140887; -.
DR UCSC; uc009ant.2; mouse.
DR CTD; 222484; -.
DR MGI; MGI:2155959; Lnx2.
DR VEuPathDB; HostDB:ENSMUSG00000016520; -.
DR eggNOG; KOG3528; Eukaryota.
DR GeneTree; ENSGT00940000158381; -.
DR HOGENOM; CLU_021213_0_0_1; -.
DR InParanoid; Q91XL2; -.
DR OMA; CLGTGTV; -.
DR OrthoDB; 615205at2759; -.
DR PhylomeDB; Q91XL2; -.
DR TreeFam; TF330709; -.
DR BioGRID-ORCS; 140887; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Lnx2; mouse.
DR PRO; PR:Q91XL2; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q91XL2; protein.
DR Bgee; ENSMUSG00000016520; Expressed in animal zygote and 197 other tissues.
DR Genevisible; Q91XL2; MM.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030165; F:PDZ domain binding; IDA:MGI.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0061351; P:neural precursor cell proliferation; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR Gene3D; 2.30.42.10; -; 4.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF00595; PDZ; 4.
DR SMART; SM00228; PDZ; 4.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF50156; SSF50156; 4.
DR PROSITE; PS50106; PDZ; 4.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..687
FT /note="Ligand of Numb protein X 2"
FT /id="PRO_0000055916"
FT DOMAIN 232..317
FT /note="PDZ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 338..421
FT /note="PDZ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 465..551
FT /note="PDZ 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 597..685
FT /note="PDZ 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT ZN_FING 51..89
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 145..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 417..454
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 207..210
FT /note="NPXY motif"
FT COMPBIAS 429..446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 207..210
FT /note="NPAF->I: Abolishes binding to NUMB protein."
FT /evidence="ECO:0000269|PubMed:11922143"
FT CONFLICT 648
FT /note="I -> M (in Ref. 1; AAK94476)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 687 AA; 75129 MW; 5B21B2D929C295D2 CRC64;
MGTTSDEMVP VEQASSTSSL DPLCFECGQQ HWARENHLYN YQGEVDDDLV CHICLQPLLQ
PLDTPCGHTF CHKCLRNFLQ EKDFCPLDRK RLHFKLCKKS SILVHKLLDK LLVLCPFSPV
CQDVMQRCDL EAHLKNRCPG ASHRRVDLER RKTSQTQTQI EGETGSTVID PPGTLPPETD
CSGTVPGERN LTPASLPVWT EEPGLDNPAF EESAAADSVQ QPLSLPEGEI TTIEIHRSNP
YIQLGISIVG GNETPLINIV IQEVYRDGVI ARDGRLLAGD QILQVNNYDI SNVSHNHARA
VLSQPCSTLQ LTVLRERRFG SRANSHADGS APRDEVFQVL LHKRDSTEQL GIKLVRRTDE
PGVFILDLLE GGLAAQDGRL NSNDRVLAIN GHDLKHGTPE LAAQIIQASG ERVNLTIARP
GKPQPSNGSR EAGAHSSSNH AQPPSHSRPG SHKDLTRCVT CQEKHITVKK EPHESLGMTV
AGGRGSKSGE LPIFVTSVPP HGCLARDGRI KRGDVLLNIN GIDLTNLSHS EAVAMLKASA
ASPAVILKAL EVQIAEEAAQ ATEEQPSAFS ENEYDASWSP SWVMWLGLPS ALHSCHDIVL
RRSYLGSWGF SIVGGYEENH TNQPFFIKTI VLGTPAYYDG RLKCGDMIVA VNGLSTVGMS
HSALVPMLKE QRNKVTLTVI CWPGSLV
