LOGH_RHOFA
ID LOGH_RHOFA Reviewed; 198 AA.
AC P46378;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Cytokinin riboside 5'-monophosphate phosphoribohydrolase;
DE EC=3.2.2.n1 {ECO:0000250|UniProtKB:O05306};
DE AltName: Full=LOG family protein ORF6 in fasciation locus;
GN Name=fas6;
OS Rhodococcus fascians.
OG Plasmid pFiD188.
OC Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX NCBI_TaxID=1828;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=D188;
RX PubMed=8169198; DOI=10.1128/jb.176.9.2492-2501.1994;
RA Crespi M., Vereecke D., Temmerman W., van Montagu M., Desomer J.;
RT "The fas operon of Rhodococcus fascians encodes new genes required for
RT efficient fasciation of host plants.";
RL J. Bacteriol. 176:2492-2501(1994).
CC -!- FUNCTION: Catalyzes the hydrolytic removal of ribose 5'-monophosphate
CC from nitrogen N6-modified adenosines, the final step of bioactive
CC cytokinin synthesis. {ECO:0000250|UniProtKB:O05306}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N(6)-(dimethylallyl)adenosine 5'-phosphate = D-ribose 5-
CC phosphate + N(6)-dimethylallyladenine; Xref=Rhea:RHEA:48560,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:17660, ChEBI:CHEBI:57526,
CC ChEBI:CHEBI:78346; EC=3.2.2.n1;
CC Evidence={ECO:0000250|UniProtKB:O05306};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-ribosyl-trans-zeatin 5'-phosphate + H2O = D-ribose 5-
CC phosphate + trans-zeatin; Xref=Rhea:RHEA:48564, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:16522, ChEBI:CHEBI:78346, ChEBI:CHEBI:87947; EC=3.2.2.n1;
CC Evidence={ECO:0000250|UniProtKB:O05306};
CC -!- INDUCTION: During the interaction with host plants.
CC {ECO:0000269|PubMed:8169198}.
CC -!- MISCELLANEOUS: The FAS-operon encodes genes involved in cytokinin
CC production and in host plant fasciation (leafy gall).
CC {ECO:0000305|PubMed:8169198}.
CC -!- SIMILARITY: Belongs to the LOG family. {ECO:0000305}.
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DR EMBL; Z29635; CAA82746.1; -; Genomic_DNA.
DR PIR; F55578; F55578.
DR RefSeq; WP_015586136.1; NZ_NPFU01000019.1.
DR RefSeq; YP_007878709.1; NC_021080.1.
DR AlphaFoldDB; P46378; -.
DR SMR; P46378; -.
DR STRING; 1443905.GCA_000761075_00035; -.
DR GeneID; 29801202; -.
DR eggNOG; COG1611; Bacteria.
DR GO; GO:0102682; F:N6-(Delta2-isopentenyl)-adenosine 5'-monophosphate phosphoribohydrolase activity; IEA:RHEA.
DR GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR005269; LOG.
DR InterPro; IPR031100; LOG_fam.
DR Pfam; PF03641; Lysine_decarbox; 1.
DR TIGRFAMs; TIGR00730; TIGR00730; 1.
PE 2: Evidence at transcript level;
KW Cytokinin biosynthesis; Hydrolase; Plasmid.
FT CHAIN 1..198
FT /note="Cytokinin riboside 5'-monophosphate
FT phosphoribohydrolase"
FT /id="PRO_0000087194"
FT BINDING 91
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B2HS63"
FT BINDING 109..110
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B2HS63"
FT BINDING 126..132
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B2HS63"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B2HS63"
SQ SEQUENCE 198 AA; 21057 MW; 597F211C65C0B2F5 CRC64;
MNLRPMPATT VSAQARPTPK SVTVFCGAMP GRGTKYGQLA EGMGRAIARS KLRLVYGGAR
VGLMGTLANA ALDSGGTVVG VIPESFTAIP EAAHHGLTEL HVVHDMHQRK ALMAELGDAF
IALPGGVGTA EEFFEVLTWS HLGLHNKPCV LLNDNEYYRP LLSYIEHAAV EGFITPATRS
RVIVCKDIEG AIAAIRSP
