LOLA1_DROME
ID LOLA1_DROME Reviewed; 891 AA.
AC P42283; Q54A11; Q9V5M2;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Longitudinals lacking protein, isoform G;
GN Name=lola {ECO:0000312|FlyBase:FBgn0283521};
GN ORFNames=CG12052 {ECO:0000312|FlyBase:FBgn0283521};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=8050351; DOI=10.1242/dev.120.6.1385;
RA Giniger E., Tietje K., Jan L.Y., Jan Y.N.;
RT "Lola encodes a putative transcription factor required for axon growth and
RT guidance in Drosophila.";
RL Development 120:1385-1398(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Canton-S;
RX PubMed=12853139; DOI=10.1016/s0378-1119(03)00554-7;
RA Ohsako T., Horiuchi T., Matsuo T., Komaya S., Aigaki T.;
RT "Drosophila lola encodes a family of BTB-transcription regulators with
RT highly variable C-terminal domains containing zinc finger motifs.";
RL Gene 311:59-69(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF ALA-107.
RX PubMed=11880341; DOI=10.1242/dev.129.6.1317;
RA Crowner D., Madden K., Goeke S., Giniger E.;
RT "Lola regulates midline crossing of CNS axons in Drosophila.";
RL Development 129:1317-1325(2002).
RN [6]
RP TRANS-SPLICING.
RX PubMed=14522953; DOI=10.1101/gad.1137303;
RA Horiuchi T., Giniger E., Aigaki T.;
RT "Alternative trans-splicing of constant and variable exons of a Drosophila
RT axon guidance gene, lola.";
RL Genes Dev. 17:2496-2501(2003).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=12538650; DOI=10.1074/jbc.m213269200;
RA Zhang W., Wang Y., Long J., Girton J., Johansen J., Johansen K.M.;
RT "A developmentally regulated splice variant from the complex lola locus
RT encoding multiple different zinc finger domain proteins interacts with the
RT chromosomal kinase JIL-1.";
RL J. Biol. Chem. 278:11696-11704(2003).
RN [8]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=12897787; DOI=10.1038/nn1105;
RA Goeke S., Greene E.A., Grant P.K., Gates M.A., Crowner D., Aigaki T.,
RA Giniger E.;
RT "Alternative splicing of lola generates 19 transcription factors
RT controlling axon guidance in Drosophila.";
RL Nat. Neurosci. 6:917-924(2003).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; THR-161; SER-162;
RP SER-168; SER-372; SER-375; SER-378; SER-696; SER-705; THR-706; SER-749;
RP SER-750 AND SER-874, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Putative transcription factor required for axon growth and
CC guidance in the central and peripheral nervous systems. Repels CNS
CC axons away from the midline by promoting the expression of the midline
CC repellent sli and its receptor robo. {ECO:0000269|PubMed:11880341,
CC ECO:0000269|PubMed:8050351}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:8050351}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=22;
CC Comment=Some isoforms may be generated by alternative trans-splicing
CC of exons sequentially encoded by the same DNA strand.;
CC Name=G; Synonyms=Long, Ohsako-T, R;
CC IsoId=P42283-1; Sequence=Displayed;
CC Name=A; Synonyms=Ohsako-D;
CC IsoId=Q7KQZ4-2; Sequence=External;
CC Name=B; Synonyms=C, Ohsako-L;
CC IsoId=Q7KQZ4-1; Sequence=External;
CC Name=D; Synonyms=E, Ohsako-F;
CC IsoId=Q7KQZ4-3; Sequence=External;
CC Name=F; Synonyms=Ohsako-I;
CC IsoId=Q867Z4-2; Sequence=External;
CC Name=H; Synonyms=Ohsako-M;
CC IsoId=P42284-3; Sequence=External;
CC Name=I; Synonyms=Ohsako-K;
CC IsoId=Q867Z4-1; Sequence=External;
CC Name=J; Synonyms=Ohsako-O;
CC IsoId=Q9V5M6-2; Sequence=External;
CC Name=K; Synonyms=Ohsako-H;
CC IsoId=Q867Z4-3; Sequence=External;
CC Name=L; Synonyms=Ohsako-C;
CC IsoId=Q7KQZ4-4; Sequence=External;
CC Name=M; Synonyms=Short, Ohsako-A;
CC IsoId=P42284-1; Sequence=External;
CC Name=N;
CC IsoId=Q9V5M3-1; Sequence=External;
CC Name=O; Synonyms=Ohsako-P;
CC IsoId=Q9V5M3-2; Sequence=External;
CC Name=P; Synonyms=Ohsako-N;
CC IsoId=Q9V5M6-1; Sequence=External;
CC Name=Q; Synonyms=Ohsako-B;
CC IsoId=Q9V5M6-3; Sequence=External;
CC Name=S;
CC IsoId=Q9V5M6-4; Sequence=External;
CC Name=T; Synonyms=U, Ohsako-J;
CC IsoId=Q867Z4-5; Sequence=External;
CC Name=V; Synonyms=Ohsako-G;
CC IsoId=P42284-2; Sequence=External;
CC Name=W; Synonyms=Ohsako-Q;
CC IsoId=Q9V5M3-3; Sequence=External;
CC Name=X; Synonyms=Ohsako-S;
CC IsoId=Q9V5M3-4; Sequence=External;
CC Name=Y; Synonyms=Ohsako-R;
CC IsoId=Q9V5M3-5; Sequence=External;
CC Name=Z; Synonyms=Ohsako-E;
CC IsoId=Q9V5M6-5; Sequence=External;
CC -!- TISSUE SPECIFICITY: Expressed in both mesoderm and ectoderm with
CC expression highest in the mesectoderm by stage 11. Becomes enriched in
CC a cluster of brain cells, in abdominal histoblasts, and in the
CC embryonic imaginal disks during later stages.
CC {ECO:0000269|PubMed:12897787}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. At
CC least one isoform is present at each developmental stage.
CC {ECO:0000269|PubMed:12538650, ECO:0000269|PubMed:12897787,
CC ECO:0000269|PubMed:8050351}.
CC -!- MISCELLANEOUS: [Isoform G]: C-terminal exons may be joined by a trans-
CC splicing event. The exons are coded by the same DNA strand.
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DR EMBL; U07607; AAA19593.1; -; mRNA.
DR EMBL; AB107291; BAC67596.1; -; mRNA.
DR EMBL; AB107311; BAC67616.1; -; mRNA.
DR EMBL; AB107331; BAC67636.1; -; mRNA.
DR EMBL; AB107351; BAC67656.1; -; mRNA.
DR EMBL; AE013599; AAF58782.2; -; Genomic_DNA.
DR EMBL; AE013599; AAO41425.2; -; Genomic_DNA.
DR RefSeq; NP_524766.2; NM_080027.6. [P42283-1]
DR RefSeq; NP_788312.2; NM_176132.4. [P42283-1]
DR AlphaFoldDB; P42283; -.
DR BioGRID; 69126; 52.
DR IntAct; P42283; 4.
DR iPTMnet; P42283; -.
DR PRIDE; P42283; -.
DR DNASU; 44548; -.
DR EnsemblMetazoa; FBtr0089347; FBpp0088381; FBgn0283521. [P42283-1]
DR EnsemblMetazoa; FBtr0089357; FBpp0088391; FBgn0283521. [P42283-1]
DR GeneID; 44548; -.
DR KEGG; dme:Dmel_CG12052; -.
DR CTD; 44548; -.
DR FlyBase; FBgn0283521; lola.
DR VEuPathDB; VectorBase:FBgn0283521; -.
DR GeneTree; ENSGT00940000174551; -.
DR HOGENOM; CLU_010740_3_0_1; -.
DR SignaLink; P42283; -.
DR BioGRID-ORCS; 44548; 1 hit in 1 CRISPR screen.
DR ChiTaRS; lola; fly.
DR GenomeRNAi; 44548; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0283521; Expressed in cleaving embryo and 52 other tissues.
DR ExpressionAtlas; P42283; baseline and differential.
DR Genevisible; P42283; DM.
DR GO; GO:0005654; C:nucleoplasm; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0016199; P:axon midline choice point recognition; IMP:UniProtKB.
DR GO; GO:0007409; P:axonogenesis; IMP:UniProtKB.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0008406; P:gonad development; IMP:FlyBase.
DR GO; GO:0035167; P:larval lymph gland hemopoiesis; IMP:FlyBase.
DR GO; GO:0007526; P:larval somatic muscle development; IMP:FlyBase.
DR GO; GO:0045476; P:nurse cell apoptotic process; IMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007464; P:R3/R4 cell fate commitment; IMP:FlyBase.
DR GO; GO:0045467; P:R7 cell development; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:FlyBase.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Metal-binding; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..891
FT /note="Longitudinals lacking protein, isoform G"
FT /id="PRO_0000047072"
FT DOMAIN 32..97
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 791..813
FT /note="C2H2-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 821..843
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 115..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 228..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 840..891
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 840..860
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 861..876
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 140
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 161
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 162
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 372
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 375
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 378
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 696
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 705
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 706
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 749
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 750
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 874
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MUTAGEN 107
FT /note="A->V: In ORE120; defective in embryonic axon
FT guidance."
FT /evidence="ECO:0000269|PubMed:11880341"
FT CONFLICT 605..607
FT /note="HTT -> QLGVVK (in Ref. 1; AAA19593 and 2; BAC67596/
FT BAC67616/BAC67636/BAC67656)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 891 AA; 96254 MW; 3047E7128C096CB5 CRC64;
MDDDQQFCLR WNNHQSTLIS VFDTLLENET LVDCTLAAEG KFLKAHKVVL SACSPYFATL
LQEQYDKHPI FILKDVKYQE LRAMMDYMYR GEVNISQDQL AALLKAAESL QIKGLSDNRT
GGGVAPKPES SGHHRGGKLS GAYTLEQTKR ARLATGGAMD TSGDVSGSRE GSSSPSRRRR
KVRRRSMEND AHDNSNSSVL QAAASNQSIL QQTGAGLAVS ALVTTQLSSG PAAGTSSQAS
STQQQQPLTS TNVTKKTESA KLTSSTAAPA SGASASAAVQ QAHLHQQQAQ TTSDAINTEN
VQAQSQGGAQ GVQGDDEDID EGSAVGGPNS ATGPNPASAS ASAVHAGVVV KQLASVVDKS
SSNHKHKIKD NSVSSVGSEM VIEPKAEYDD DAHDENVEDL TLDEEDMTME ELDQTAGTSQ
GGEGSSQTYA TWQHDRSQDE LGLMAQDAQQ RDPQDLSRKE NTAPDVASTA EIQRSFQRSI
LNGKQRDEQK IQLPGSRRKR LSVTEVSDML FEFYKTKSAK VPKAEQPHRQ VSPTSGEILD
PSTISAIAVY GTASETASKN LNADEVMRVQ NATATRVVGA AAGAAASFHP RPKYTLKTAA
SSTEHTTAIP TSVLVANSAA ALTPKPQAAV IAEALMRNGL HNFQQQLRAQ EILRQQTPHR
RIKEENDVEI AGGDITPTKI LENLLRKQQE RDLRHSECEN EPGYSTEDDE EGRYHAFDDI
HLMEQSGGKF GNNSGMGMFN ANAHGGSASS ILDAHQAFRN LEFTLSDYGG SSSNGSTTSP
NGIGLDGEPV YECRHCGKKY RWKSTLRRHE NVECGGKEPS HQCPYCPYKS KQRGNLGVHV
RKHHTDLPQL PSKRRSKYSM NRENGMSGSM SDDSQGKLII DFNGKGELET K
