LOLA2_DROME
ID LOLA2_DROME Reviewed; 549 AA.
AC P42284; Q867M9; Q867R1; Q868A5;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Longitudinals lacking protein, isoforms H/M/V;
GN Name=lola {ECO:0000312|FlyBase:FBgn0283521};
GN ORFNames=CG12052 {ECO:0000312|FlyBase:FBgn0283521};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=8050351; DOI=10.1242/dev.120.6.1385;
RA Giniger E., Tietje K., Jan L.Y., Jan Y.N.;
RT "Lola encodes a putative transcription factor required for axon growth and
RT guidance in Drosophila.";
RL Development 120:1385-1398(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS H; M AND V).
RC STRAIN=Canton-S; TISSUE=Embryo, Larva, and Pupae;
RX PubMed=12853139; DOI=10.1016/s0378-1119(03)00554-7;
RA Ohsako T., Horiuchi T., Matsuo T., Komaya S., Aigaki T.;
RT "Drosophila lola encodes a family of BTB-transcription regulators with
RT highly variable C-terminal domains containing zinc finger motifs.";
RL Gene 311:59-69(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF ALA-107.
RX PubMed=11880341; DOI=10.1242/dev.129.6.1317;
RA Crowner D., Madden K., Goeke S., Giniger E.;
RT "Lola regulates midline crossing of CNS axons in Drosophila.";
RL Development 129:1317-1325(2002).
RN [6]
RP TRANS-SPLICING.
RX PubMed=14522953; DOI=10.1101/gad.1137303;
RA Horiuchi T., Giniger E., Aigaki T.;
RT "Alternative trans-splicing of constant and variable exons of a Drosophila
RT axon guidance gene, lola.";
RL Genes Dev. 17:2496-2501(2003).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=12538650; DOI=10.1074/jbc.m213269200;
RA Zhang W., Wang Y., Long J., Girton J., Johansen J., Johansen K.M.;
RT "A developmentally regulated splice variant from the complex lola locus
RT encoding multiple different zinc finger domain proteins interacts with the
RT chromosomal kinase JIL-1.";
RL J. Biol. Chem. 278:11696-11704(2003).
RN [8]
RP DEVELOPMENTAL STAGE.
RX PubMed=12897787; DOI=10.1038/nn1105;
RA Goeke S., Greene E.A., Grant P.K., Gates M.A., Crowner D., Aigaki T.,
RA Giniger E.;
RT "Alternative splicing of lola generates 19 transcription factors
RT controlling axon guidance in Drosophila.";
RL Nat. Neurosci. 6:917-924(2003).
CC -!- FUNCTION: Putative transcription factor required for axon growth and
CC guidance in the central and peripheral nervous systems. Repels CNS
CC axons away from the midline by promoting the expression of the midline
CC repellent sli and its receptor robo. {ECO:0000269|PubMed:11880341,
CC ECO:0000269|PubMed:8050351}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:8050351}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=22;
CC Comment=Some isoforms may be generated by alternative trans-splicing
CC of exons sequentially encoded by the same DNA strand.;
CC Name=V; Synonyms=Ohsako-G;
CC IsoId=P42284-2; Sequence=Displayed;
CC Name=H; Synonyms=Ohsako-M;
CC IsoId=P42284-3; Sequence=VSP_015404, VSP_015406;
CC Name=M; Synonyms=Short, Ohsako-A;
CC IsoId=P42284-1; Sequence=VSP_015403, VSP_015405;
CC Name=A; Synonyms=Ohsako-D;
CC IsoId=Q7KQZ4-2; Sequence=External;
CC Name=B; Synonyms=C, Ohsako-L;
CC IsoId=Q7KQZ4-1; Sequence=External;
CC Name=D; Synonyms=E, Ohsako-F;
CC IsoId=Q7KQZ4-3; Sequence=External;
CC Name=F; Synonyms=Ohsako-I;
CC IsoId=Q867Z4-2; Sequence=External;
CC Name=G; Synonyms=Long, Ohsako-T, R;
CC IsoId=P42283-1; Sequence=External;
CC Name=I; Synonyms=Ohsako-K;
CC IsoId=Q867Z4-1; Sequence=External;
CC Name=J; Synonyms=Ohsako-O;
CC IsoId=Q9V5M6-2; Sequence=External;
CC Name=K; Synonyms=Ohsako-H;
CC IsoId=Q867Z4-3; Sequence=External;
CC Name=L; Synonyms=Ohsako-C;
CC IsoId=Q7KQZ4-4; Sequence=External;
CC Name=N;
CC IsoId=Q9V5M3-1; Sequence=External;
CC Name=O; Synonyms=Ohsako-P;
CC IsoId=Q9V5M3-2; Sequence=External;
CC Name=P; Synonyms=Ohsako-N;
CC IsoId=Q9V5M6-1; Sequence=External;
CC Name=Q; Synonyms=Ohsako-B;
CC IsoId=Q9V5M6-3; Sequence=External;
CC Name=S;
CC IsoId=Q9V5M6-4; Sequence=External;
CC Name=T; Synonyms=U, Ohsako-J;
CC IsoId=Q867Z4-5; Sequence=External;
CC Name=W; Synonyms=Ohsako-Q;
CC IsoId=Q9V5M3-3; Sequence=External;
CC Name=X; Synonyms=Ohsako-S;
CC IsoId=Q9V5M3-4; Sequence=External;
CC Name=Y; Synonyms=Ohsako-R;
CC IsoId=Q9V5M3-5; Sequence=External;
CC Name=Z; Synonyms=Ohsako-E;
CC IsoId=Q9V5M6-5; Sequence=External;
CC -!- TISSUE SPECIFICITY: Mostly neuronal. {ECO:0000269|PubMed:8050351}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. At
CC least one isoform is present at each developmental stage.
CC {ECO:0000269|PubMed:12538650, ECO:0000269|PubMed:12897787,
CC ECO:0000269|PubMed:8050351}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U07606; AAA19592.1; -; mRNA.
DR EMBL; AB107272; BAC67577.1; -; mRNA.
DR EMBL; AB107278; BAC67583.1; -; mRNA.
DR EMBL; AB107284; BAC67589.1; -; mRNA.
DR EMBL; AB107292; BAC67597.1; -; mRNA.
DR EMBL; AB107298; BAC67603.1; -; mRNA.
DR EMBL; AB107304; BAC67609.1; -; mRNA.
DR EMBL; AB107312; BAC67617.1; -; mRNA.
DR EMBL; AB107318; BAC67623.1; -; mRNA.
DR EMBL; AB107324; BAC67629.1; -; mRNA.
DR EMBL; AB107332; BAC67637.1; -; mRNA.
DR EMBL; AB107338; BAC67643.1; -; mRNA.
DR EMBL; AB107344; BAC67649.1; -; mRNA.
DR EMBL; AE013599; AAF58777.2; -; Genomic_DNA.
DR EMBL; AE013599; AAO41431.1; -; Genomic_DNA.
DR EMBL; AE013599; AAS64877.1; -; Genomic_DNA.
DR RefSeq; NP_724945.1; NM_170617.5. [P42284-3]
DR RefSeq; NP_788320.1; NM_176140.4. [P42284-1]
DR RefSeq; NP_995807.1; NM_206085.4. [P42284-2]
DR AlphaFoldDB; P42284; -.
DR SMR; P42284; -.
DR BioGRID; 69126; 52.
DR DNASU; 44548; -.
DR EnsemblMetazoa; FBtr0089346; FBpp0088380; FBgn0283521. [P42284-3]
DR EnsemblMetazoa; FBtr0089362; FBpp0088396; FBgn0283521. [P42284-1]
DR EnsemblMetazoa; FBtr0089363; FBpp0088949; FBgn0283521. [P42284-2]
DR GeneID; 44548; -.
DR CTD; 44548; -.
DR FlyBase; FBgn0283521; lola.
DR VEuPathDB; VectorBase:FBgn0283521; -.
DR GeneTree; ENSGT00940000174551; -.
DR BioGRID-ORCS; 44548; 1 hit in 1 CRISPR screen.
DR ChiTaRS; lola; fly.
DR GenomeRNAi; 44548; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0283521; Expressed in cleaving embryo and 52 other tissues.
DR ExpressionAtlas; P42284; baseline and differential.
DR Genevisible; P42284; DM.
DR GO; GO:0005654; C:nucleoplasm; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:FlyBase.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0016199; P:axon midline choice point recognition; IMP:UniProtKB.
DR GO; GO:0007409; P:axonogenesis; IMP:UniProtKB.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0008406; P:gonad development; IMP:FlyBase.
DR GO; GO:0035167; P:larval lymph gland hemopoiesis; IMP:FlyBase.
DR GO; GO:0007526; P:larval somatic muscle development; IMP:FlyBase.
DR GO; GO:0045476; P:nurse cell apoptotic process; IMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007464; P:R3/R4 cell fate commitment; IMP:FlyBase.
DR GO; GO:0045467; P:R7 cell development; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:FlyBase.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; Neurogenesis;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..549
FT /note="Longitudinals lacking protein, isoforms H/M/V"
FT /id="PRO_0000047073"
FT DOMAIN 32..97
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REGION 115..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 228..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 455..518
FT /note="DVSTNQTVVLPHYSIYHYYSNIYYLLSHTTIYEADRTVSVSCPGKLNCLPQR
FT NDLQETKSVTVL -> DEAGQNEGGESRIRVRNWLMLADKSIIGKSSDEPSVLHIVLLL
FT STHRHIISFLLIIQSFIDKIY (in isoform H)"
FT /evidence="ECO:0000303|PubMed:12853139"
FT /id="VSP_015404"
FT VAR_SEQ 455..465
FT /note="DVSTNQTVVLP -> GECLLPLKSII (in isoform M)"
FT /evidence="ECO:0000303|PubMed:12853139,
FT ECO:0000303|PubMed:8050351"
FT /id="VSP_015403"
FT VAR_SEQ 466..549
FT /note="Missing (in isoform M)"
FT /evidence="ECO:0000303|PubMed:12853139,
FT ECO:0000303|PubMed:8050351"
FT /id="VSP_015405"
FT VAR_SEQ 519..549
FT /note="Missing (in isoform H)"
FT /evidence="ECO:0000303|PubMed:12853139"
FT /id="VSP_015406"
FT MUTAGEN 107
FT /note="A->V: In ORE120; defective in embryonic axon
FT guidance."
FT /evidence="ECO:0000269|PubMed:11880341"
FT CONFLICT P42284-1:465
FT /note="I -> YEL (in Ref. 1; AAA19592)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 549 AA; 59323 MW; 0FB6C88602ADAB49 CRC64;
MDDDQQFCLR WNNHQSTLIS VFDTLLENET LVDCTLAAEG KFLKAHKVVL SACSPYFATL
LQEQYDKHPI FILKDVKYQE LRAMMDYMYR GEVNISQDQL AALLKAAESL QIKGLSDNRT
GGGVAPKPES SGHHRGGKLS GAYTLEQTKR ARLATGGAMD TSGDVSGSRE GSSSPSRRRR
KVRRRSMEND AHDNSNSSVL QAAASNQSIL QQTGAGLAVS ALVTTQLSSG PAAGTSSQAS
STQQQQPLTS TNVTKKTESA KLTSSTAAPA SGASASAAVQ QAHLHQQQAQ TTSDAINTEN
VQAQSQGGAQ GVQGDDEDID EGSAVGGPNS ATGPNPASAS ASAVHAGVVV KQLASVVDKS
SSNHKHKIKD NSVSSVGSEM VIEPKAEYDD DAHDENVEDL TLDEEDMTME ELDQTAGTSQ
GGEGSSQTYA TWQHDRSQDE LGLMAQDAQQ RDPQDVSTNQ TVVLPHYSIY HYYSNIYYLL
SHTTIYEADR TVSVSCPGKL NCLPQRNDLQ ETKSVTVLYT IHFFLYILMI YIFVLCKILP
RIVFVWVST
