LOLB_ECOLI
ID LOLB_ECOLI Reviewed; 207 AA.
AC P61320; P24208; Q46753;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Outer-membrane lipoprotein LolB;
DE Flags: Precursor;
GN Name=lolB; Synonyms=hemM, ychC; OrderedLocusNames=b1209, JW1200;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1427085; DOI=10.1016/0378-1119(92)90170-t;
RA Ikemi M., Murakami K., Hashimoto M., Murooka Y.;
RT "Cloning and characterization of genes involved in the biosynthesis of
RT delta-aminolevulinic acid in Escherichia coli.";
RL Gene 121:127-132(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7679718; DOI=10.1099/00221287-139-2-259;
RA Post D.A., Hove-Jensen B., Switzer R.L.;
RT "Characterization of the hemA-prs region of the Escherichia coli and
RT Salmonella typhimurium chromosomes: identification of two open reading
RT frames and implications for prs expression.";
RL J. Gen. Microbiol. 139:259-266(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7543480; DOI=10.1128/jb.177.15.4488-4500.1995;
RA Strohmaier H., Remler P., Renner W., Hoegenauer G.;
RT "Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic
RT acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is
RT growth phase regulated primarily at the transcriptional level in
RT Escherichia coli K-12.";
RL J. Bacteriol. 177:4488-4500(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [7]
RP CHARACTERIZATION, DIACYLGLYCEROL AT CYS-22, PALMITOYLATION AT CYS-22, AND
RP PARTIAL PROTEIN SEQUENCE.
RX PubMed=9384574; DOI=10.1093/emboj/16.23.6947;
RA Matsuyama S., Yokota N., Tokuda H.;
RT "A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA
RT (p20)-dependent localization of lipoproteins to the outer membrane of
RT Escherichia coli.";
RL EMBO J. 16:6947-6955(1997).
RN [8]
RP IMPORTANCE FOR VIABILITY.
RC STRAIN=K12;
RX PubMed=11673422; DOI=10.1128/jb.183.22.6538-6542.2001;
RA Tanaka K., Matsuyama S., Tokuda H.;
RT "Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for
RT Escherichia coli and causes accumulation of lipoprotein localization
RT intermediates in the periplasm.";
RL J. Bacteriol. 183:6538-6542(2001).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX PubMed=12839983; DOI=10.1093/emboj/cdg324;
RA Takeda K., Miyatake H., Yokota N., Matsuyama S., Tokuda H., Miki K.;
RT "Crystal structures of bacterial lipoprotein localization factors, LolA and
RT LolB.";
RL EMBO J. 22:3199-3209(2003).
CC -!- FUNCTION: Plays a critical role in the incorporation of lipoproteins in
CC the outer membrane after they are released by the LolA protein.
CC Essential for E.coli viability.
CC -!- SUBUNIT: Monomer.
CC -!- INTERACTION:
CC P61320; P61316: lolA; NbExp=2; IntAct=EBI-1122794, EBI-553532;
CC P61320; P0A912: pal; NbExp=2; IntAct=EBI-1122794, EBI-1124760;
CC -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor.
CC -!- SIMILARITY: Belongs to the LolB family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be involved in delta-aminolevulinic
CC acid biosynthesis. {ECO:0000305|PubMed:1427085}.
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DR EMBL; M77237; AAA24433.1; -; Genomic_DNA.
DR EMBL; D10264; BAA01105.1; -; Genomic_DNA.
DR EMBL; U18555; AAC43435.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74293.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA36067.1; -; Genomic_DNA.
DR PIR; A47706; A47706.
DR RefSeq; NP_415727.1; NC_000913.3.
DR RefSeq; WP_001130692.1; NZ_SSZK01000010.1.
DR PDB; 1IWM; X-ray; 1.90 A; A/B=22-207.
DR PDB; 1IWN; X-ray; 2.20 A; A=22-207.
DR PDBsum; 1IWM; -.
DR PDBsum; 1IWN; -.
DR AlphaFoldDB; P61320; -.
DR SMR; P61320; -.
DR BioGRID; 4260814; 301.
DR DIP; DIP-35933N; -.
DR IntAct; P61320; 9.
DR MINT; P61320; -.
DR STRING; 511145.b1209; -.
DR DrugBank; DB02078; Triglyme.
DR TCDB; 1.B.46.1.1; the outer membrane lolab lipoprotein insertion apparatus (lolab) family.
DR jPOST; P61320; -.
DR PaxDb; P61320; -.
DR PRIDE; P61320; -.
DR EnsemblBacteria; AAC74293; AAC74293; b1209.
DR EnsemblBacteria; BAA36067; BAA36067; BAA36067.
DR GeneID; 66674971; -.
DR GeneID; 945775; -.
DR KEGG; ecj:JW1200; -.
DR KEGG; eco:b1209; -.
DR PATRIC; fig|1411691.4.peg.1075; -.
DR EchoBASE; EB1270; -.
DR eggNOG; COG3017; Bacteria.
DR HOGENOM; CLU_092816_1_1_6; -.
DR InParanoid; P61320; -.
DR OMA; YQTRGSF; -.
DR PhylomeDB; P61320; -.
DR BioCyc; EcoCyc:EG11293-MON; -.
DR BioCyc; MetaCyc:EG11293-MON; -.
DR EvolutionaryTrace; P61320; -.
DR PRO; PR:P61320; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0036406; C:anchored component of periplasmic side of cell outer membrane; IDA:EcoCyc.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0044874; P:lipoprotein localization to outer membrane; IDA:CACAO.
DR GO; GO:0042157; P:lipoprotein metabolic process; IMP:EcoCyc.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd16326; LolB; 1.
DR HAMAP; MF_00233; LolB; 1.
DR InterPro; IPR029046; LolA/LolB/LppX.
DR InterPro; IPR004565; OM_lipoprot_LolB.
DR Pfam; PF03550; LolB; 1.
DR SUPFAM; SSF89392; SSF89392; 1.
DR TIGRFAMs; TIGR00548; lolB; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Chaperone; Direct protein sequencing;
KW Lipoprotein; Membrane; Palmitate; Protein transport; Reference proteome;
KW Signal; Transport.
FT SIGNAL 1..21
FT CHAIN 22..207
FT /note="Outer-membrane lipoprotein LolB"
FT /id="PRO_0000018296"
FT LIPID 22
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:9384574"
FT LIPID 22
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305|PubMed:9384574"
FT CONFLICT 12
FT /note="L -> V (in Ref. 1; BAA01105)"
FT /evidence="ECO:0000305"
FT CONFLICT 41..42
FT /note="QH -> HD (in Ref. 3; AAC43435)"
FT /evidence="ECO:0000305"
FT CONFLICT 176..207
FT /note="YDTKTQPAMPANMELTDGGQRIKLKMDNWIVK -> MTPKRNLRCQPIWNSP
FT TVVNASS (in Ref. 3; AAC43435)"
FT /evidence="ECO:0000305"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:1IWM"
FT HELIX 37..47
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 51..61
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 66..76
FT /evidence="ECO:0007829|PDB:1IWM"
FT TURN 77..79
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 80..86
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 92..99
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 112..116
FT /evidence="ECO:0007829|PDB:1IWM"
FT HELIX 118..126
FT /evidence="ECO:0007829|PDB:1IWM"
FT HELIX 132..138
FT /evidence="ECO:0007829|PDB:1IWM"
FT TURN 139..141
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 149..151
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 157..166
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 168..173
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 180..182
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 187..191
FT /evidence="ECO:0007829|PDB:1IWM"
FT STRAND 196..207
FT /evidence="ECO:0007829|PDB:1IWM"
SQ SEQUENCE 207 AA; 23551 MW; C517F6E7353B8551 CRC64;
MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN QYQTRGAFAY
ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP GNVQLVDNKG QRYTADDAEE
MIGKLTGMPI PLNSLRQWIL GLPGDATDYK LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT
QPAMPANMEL TDGGQRIKLK MDNWIVK
