LOLC_ECOLI
ID LOLC_ECOLI Reviewed; 399 AA.
AC P0ADC3; P75956; Q9R7N7;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Lipoprotein-releasing system transmembrane protein LolC;
GN Name=lolC; Synonyms=ycfU; OrderedLocusNames=b1116, JW5161;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEIN SEQUENCE OF 1-10, AND CHARACTERIZATION.
RX PubMed=10783239; DOI=10.1038/35008635;
RA Yakushi T., Masuda K., Narita S., Matsuyama S., Tokuda H.;
RT "A new ABC transporter mediating the detachment of lipid-modified proteins
RT from membranes.";
RL Nat. Cell Biol. 2:212-218(2000).
CC -!- FUNCTION: Part of an ATP-dependent transport system LolCDE responsible
CC for the release of lipoproteins targeted to the outer membrane from the
CC inner membrane. Such a release is dependent of the sorting-signal
CC (absence of an Asp at position 2 of the mature lipoprotein) and of
CC LolA.
CC -!- INTERACTION:
CC P0ADC3; P61316: lolA; NbExp=4; IntAct=EBI-15765497, EBI-553532;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ABC-4 integral membrane protein family.
CC LolC/E subfamily. {ECO:0000305}.
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DR EMBL; U00096; AAC74200.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35936.2; -; Genomic_DNA.
DR PIR; A64856; A64856.
DR RefSeq; NP_415634.1; NC_000913.3.
DR RefSeq; WP_000284714.1; NZ_STEB01000016.1.
DR PDB; 5NAA; X-ray; 1.88 A; A/B=48-266.
DR PDB; 6F3Z; X-ray; 2.00 A; A/C=48-266.
DR PDB; 6F49; X-ray; 2.02 A; A/B/C/D=48-166, A/B/C/D=180-266.
DR PDB; 7ARH; EM; 3.30 A; C=1-399.
DR PDB; 7ARI; EM; 3.40 A; C=1-399.
DR PDB; 7ARJ; EM; 3.20 A; C=1-399.
DR PDB; 7ARK; EM; 4.10 A; C=1-399.
DR PDB; 7ARL; EM; 3.20 A; C=1-399.
DR PDB; 7ARM; EM; 3.60 A; C=1-399.
DR PDB; 7MDX; EM; 3.80 A; A=2-394.
DR PDB; 7MDY; EM; 3.50 A; A=1-399.
DR PDBsum; 5NAA; -.
DR PDBsum; 6F3Z; -.
DR PDBsum; 6F49; -.
DR PDBsum; 7ARH; -.
DR PDBsum; 7ARI; -.
DR PDBsum; 7ARJ; -.
DR PDBsum; 7ARK; -.
DR PDBsum; 7ARL; -.
DR PDBsum; 7ARM; -.
DR PDBsum; 7MDX; -.
DR PDBsum; 7MDY; -.
DR AlphaFoldDB; P0ADC3; -.
DR SMR; P0ADC3; -.
DR BioGRID; 4261911; 381.
DR ComplexPortal; CPX-4262; LolCDE lipoprotein ABC transporter complex.
DR DIP; DIP-58651N; -.
DR IntAct; P0ADC3; 3.
DR STRING; 511145.b1116; -.
DR TCDB; 3.A.1.125.1; the atp-binding cassette (abc) superfamily.
DR jPOST; P0ADC3; -.
DR PaxDb; P0ADC3; -.
DR PRIDE; P0ADC3; -.
DR EnsemblBacteria; AAC74200; AAC74200; b1116.
DR EnsemblBacteria; BAA35936; BAA35936; BAA35936.
DR GeneID; 66670618; -.
DR GeneID; 945673; -.
DR KEGG; ecj:JW5161; -.
DR KEGG; eco:b1116; -.
DR PATRIC; fig|1411691.4.peg.1151; -.
DR EchoBASE; EB3213; -.
DR eggNOG; COG4591; Bacteria.
DR HOGENOM; CLU_000604_8_1_6; -.
DR InParanoid; P0ADC3; -.
DR OMA; GWWQQNG; -.
DR PhylomeDB; P0ADC3; -.
DR BioCyc; EcoCyc:YCFU-MON; -.
DR BioCyc; MetaCyc:YCFU-MON; -.
DR PRO; PR:P0ADC3; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IPI:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0098797; C:plasma membrane protein complex; IDA:EcoCyc.
DR GO; GO:0140306; F:lipoprotein releasing activity; IDA:EcoCyc.
DR GO; GO:0044874; P:lipoprotein localization to outer membrane; IDA:EcoCyc.
DR GO; GO:0042953; P:lipoprotein transport; IEA:InterPro.
DR GO; GO:0089705; P:protein localization to outer membrane; IMP:CACAO.
DR InterPro; IPR003838; ABC3_permease_dom.
DR InterPro; IPR011925; LolCE_TM.
DR InterPro; IPR025857; MacB_PCD.
DR Pfam; PF02687; FtsX; 1.
DR Pfam; PF12704; MacB_PCD; 1.
DR TIGRFAMs; TIGR02212; lolCE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane;
KW Direct protein sequencing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..399
FT /note="Lipoprotein-releasing system transmembrane protein
FT LolC"
FT /id="PRO_0000201810"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TURN 6..13
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 19..22
FT /evidence="ECO:0007829|PDB:7ARI"
FT HELIX 23..27
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 51..58
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 65..73
FT /evidence="ECO:0007829|PDB:5NAA"
FT TURN 77..79
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 82..84
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 88..104
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 109..117
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 126..128
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 129..131
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:5NAA"
FT TURN 139..141
FT /evidence="ECO:0007829|PDB:6F3Z"
FT STRAND 143..147
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 148..154
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:7ARI"
FT STRAND 161..171
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 174..176
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 178..190
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 195..198
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 200..204
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 205..211
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 219..227
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 229..231
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:5NAA"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 244..248
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 249..251
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 254..266
FT /evidence="ECO:0007829|PDB:5NAA"
FT HELIX 273..292
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 295..303
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 307..311
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 313..317
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 320..338
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 340..344
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 353..355
FT /evidence="ECO:0007829|PDB:7ARI"
FT HELIX 362..378
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 381..388
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 392..394
FT /evidence="ECO:0007829|PDB:7ARJ"
SQ SEQUENCE 399 AA; 43264 MW; A2BA2D6AF797A09A CRC64;
MYQPVALFIG LRYMRGRAAD RFGRFVSWLS TIGITLGVMA LVTVLSVMNG FERELQNNIL
GLMPQAILSS EHGSLNPQQL PETAVKLDGV NRVAPITTGD VVLQSARSVA VGVMLGIDPA
QKDPLTPYLV NVKQTDLEPG KYNVILGEQL ASQLGVNRGD QIRVMVPSAS QFTPMGRIPS
QRLFNVIGTF AANSEVDGYE MLVNIEDASR LMRYPAGNIT GWRLWLDEPL KVDSLSQQKL
PEGSKWQDWR DRKGELFQAV RMEKNMMGLL LSLIVAVAAF NIITSLGLMV MEKQGEVAIL
QTQGLTPRQI MMVFMVQGAS AGIIGAILGA ALGALLASQL NNLMPIIGVL LDGAALPVAI
EPLQVIVIAL VAMAIALLST LYPSWRAAAT QPAEALRYE
