LOLD_ECOLI
ID LOLD_ECOLI Reviewed; 233 AA.
AC P75957; Q9R7N6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Lipoprotein-releasing system ATP-binding protein LolD {ECO:0000255|HAMAP-Rule:MF_01708};
DE EC=7.6.2.- {ECO:0000255|HAMAP-Rule:MF_01708};
GN Name=lolD {ECO:0000255|HAMAP-Rule:MF_01708}; Synonyms=ycfV;
GN OrderedLocusNames=b1117, JW5162;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEIN SEQUENCE OF 1-11, MUTAGENESIS OF GLY-42, AND CHARACTERIZATION.
RX PubMed=10783239; DOI=10.1038/35008635;
RA Yakushi T., Masuda K., Narita S., Matsuyama S., Tokuda H.;
RT "A new ABC transporter mediating the detachment of lipid-modified proteins
RT from membranes.";
RL Nat. Cell Biol. 2:212-218(2000).
RN [5]
RP CHARACTERIZATION, AND SUBUNIT.
RX PubMed=11844772; DOI=10.1128/jb.184.5.1417-1422.2002;
RA Narita S., Tanaka K., Matsuyama S., Tokuda H.;
RT "Disruption of lolCDE, encoding an ATP-binding cassette transporter, is
RT lethal for Escherichia coli and prevents release of lipoproteins from the
RT inner membrane.";
RL J. Bacteriol. 184:1417-1422(2002).
CC -!- FUNCTION: Part of the ABC transporter complex LolCDE involved in the
CC translocation of mature outer membrane-directed lipoproteins, from the
CC inner membrane to the periplasmic chaperone, LolA. Responsible for the
CC formation of the LolA-lipoprotein complex in an ATP-dependent manner.
CC Such a release is dependent of the sorting-signal (absence of an Asp at
CC position 2 of the mature lipoprotein) and of LolA.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LolD) and
CC two transmembrane proteins (LolC and LolE). {ECO:0000255|HAMAP-
CC Rule:MF_01708, ECO:0000269|PubMed:11844772}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Lipoprotein
CC translocase (TC 3.A.1.125) family. {ECO:0000255|HAMAP-Rule:MF_01708}.
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DR EMBL; U00096; AAC74201.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35937.2; -; Genomic_DNA.
DR PIR; B64856; B64856.
DR RefSeq; NP_415635.4; NC_000913.3.
DR RefSeq; WP_001033694.1; NZ_SSZK01000019.1.
DR PDB; 7ARH; EM; 3.30 A; D/F=1-233.
DR PDB; 7ARI; EM; 3.40 A; D/F=1-233.
DR PDB; 7ARJ; EM; 3.20 A; D/F=1-233.
DR PDB; 7ARK; EM; 4.10 A; D/F=1-233.
DR PDB; 7ARL; EM; 3.20 A; D/F=1-233.
DR PDB; 7ARM; EM; 3.60 A; D/F=1-233.
DR PDB; 7MDX; EM; 3.80 A; C/D=4-221.
DR PDB; 7MDY; EM; 3.50 A; C/D=3-228.
DR PDBsum; 7ARH; -.
DR PDBsum; 7ARI; -.
DR PDBsum; 7ARJ; -.
DR PDBsum; 7ARK; -.
DR PDBsum; 7ARL; -.
DR PDBsum; 7ARM; -.
DR PDBsum; 7MDX; -.
DR PDBsum; 7MDY; -.
DR AlphaFoldDB; P75957; -.
DR SMR; P75957; -.
DR BioGRID; 4260088; 228.
DR BioGRID; 850042; 7.
DR ComplexPortal; CPX-4262; LolCDE lipoprotein ABC transporter complex.
DR DIP; DIP-10115N; -.
DR IntAct; P75957; 15.
DR STRING; 511145.b1117; -.
DR TCDB; 3.A.1.125.1; the atp-binding cassette (abc) superfamily.
DR jPOST; P75957; -.
DR PaxDb; P75957; -.
DR PRIDE; P75957; -.
DR EnsemblBacteria; AAC74201; AAC74201; b1117.
DR EnsemblBacteria; BAA35937; BAA35937; BAA35937.
DR GeneID; 66670617; -.
DR GeneID; 945670; -.
DR KEGG; ecj:JW5162; -.
DR KEGG; eco:b1117; -.
DR PATRIC; fig|1411691.4.peg.1150; -.
DR EchoBASE; EB3214; -.
DR eggNOG; COG1136; Bacteria.
DR HOGENOM; CLU_000604_1_22_6; -.
DR InParanoid; P75957; -.
DR OMA; FVYQSHR; -.
DR PhylomeDB; P75957; -.
DR BioCyc; EcoCyc:YCFV-MON; -.
DR BioCyc; MetaCyc:YCFV-MON; -.
DR PRO; PR:P75957; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IPI:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:CACAO.
DR GO; GO:0098797; C:plasma membrane protein complex; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR GO; GO:0140306; F:lipoprotein releasing activity; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0044874; P:lipoprotein localization to outer membrane; IDA:EcoCyc.
DR GO; GO:0042953; P:lipoprotein transport; IEA:InterPro.
DR GO; GO:0089705; P:protein localization to outer membrane; IDA:CACAO.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR CDD; cd03255; ABC_MJ0796_LolCDE_FtsE; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015854; ABC_transpr_LolD.
DR InterPro; IPR011924; LolD_lipo_ATP-bd.
DR InterPro; IPR017911; MacB_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24220:SF654; PTHR24220:SF654; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02211; LolD_lipo_ex; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51244; LOLD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell inner membrane; Cell membrane;
KW Direct protein sequencing; Membrane; Nucleotide-binding;
KW Reference proteome; Translocase; Transport.
FT CHAIN 1..233
FT /note="Lipoprotein-releasing system ATP-binding protein
FT LolD"
FT /id="PRO_0000092434"
FT DOMAIN 6..233
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01708"
FT BINDING 42..49
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01708"
FT MUTAGEN 42
FT /note="G->D: Loss of lipoprotein release when
FT overexpressed."
FT /evidence="ECO:0000269|PubMed:10783239"
FT STRAND 5..9
FT /evidence="ECO:0007829|PDB:7ARH"
FT STRAND 14..17
FT /evidence="ECO:0007829|PDB:7ARL"
FT STRAND 20..23
FT /evidence="ECO:0007829|PDB:7ARL"
FT STRAND 29..32
FT /evidence="ECO:0007829|PDB:7ARH"
FT STRAND 37..41
FT /evidence="ECO:0007829|PDB:7ARH"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 48..54
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 62..65
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 78..87
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 88..92
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 104..108
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 110..114
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 119..121
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 122..133
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 137..140
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:7ARH"
FT HELIX 148..159
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 160..162
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 165..170
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 172..176
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 178..183
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 185..190
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 191..194
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 204..208
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 210..214
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 217..219
FT /evidence="ECO:0007829|PDB:7ARL"
SQ SEQUENCE 233 AA; 25438 MW; 1C305476182F1EFD CRC64;
MNKILLQCDN LCKRYQEGSV QTDVLHNVSF SVGEGEMMAI VGSSGSGKST LLHLLGGLDT
PTSGDVIFNG QPMSKLSSAA KAELRNQKLG FIYQFHHLLP DFTALENVAM PLLIGKKKPA
EINSRALEML KAVGLDHRAN HRPSELSGGE RQRVAIARAL VNNPRLVLAD EPTGNLDARN
ADSIFQLLGE LNRLQGTAFL VVTHDLQLAK RMSRQLEMRD GRLTAELSLM GAE
