LOLE_ECOLI
ID LOLE_ECOLI Reviewed; 414 AA.
AC P75958;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Lipoprotein-releasing system transmembrane protein LolE;
GN Name=lolE; Synonyms=ycfW; OrderedLocusNames=b1118, JW1104;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEIN SEQUENCE OF 2-11, AND CHARACTERIZATION.
RX PubMed=10783239; DOI=10.1038/35008635;
RA Yakushi T., Masuda K., Narita S., Matsuyama S., Tokuda H.;
RT "A new ABC transporter mediating the detachment of lipid-modified proteins
RT from membranes.";
RL Nat. Cell Biol. 2:212-218(2000).
CC -!- FUNCTION: Part of an ATP-dependent transport system LolCDE responsible
CC for the release of lipoproteins targeted to the outer membrane from the
CC inner membrane. Such a release is dependent of the sorting-signal
CC (absence of an Asp at position 2 of the mature lipoprotein) and of
CC LolA.
CC -!- INTERACTION:
CC P75958; P0A912: pal; NbExp=2; IntAct=EBI-1125190, EBI-1124760;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ABC-4 integral membrane protein family.
CC LolC/E subfamily. {ECO:0000305}.
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DR EMBL; U00096; AAC74202.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35938.1; -; Genomic_DNA.
DR PIR; C64856; C64856.
DR RefSeq; NP_415636.1; NC_000913.3.
DR RefSeq; WP_001251348.1; NZ_SSZK01000019.1.
DR PDB; 7ARH; EM; 3.30 A; E=1-414.
DR PDB; 7ARI; EM; 3.40 A; E=1-414.
DR PDB; 7ARJ; EM; 3.20 A; E=1-414.
DR PDB; 7ARK; EM; 4.10 A; E=1-414.
DR PDB; 7ARL; EM; 3.20 A; E=1-414.
DR PDB; 7ARM; EM; 3.60 A; E=1-414.
DR PDB; 7MDX; EM; 3.80 A; B=6-408.
DR PDB; 7MDY; EM; 3.50 A; B=1-414.
DR PDBsum; 7ARH; -.
DR PDBsum; 7ARI; -.
DR PDBsum; 7ARJ; -.
DR PDBsum; 7ARK; -.
DR PDBsum; 7ARL; -.
DR PDBsum; 7ARM; -.
DR PDBsum; 7MDX; -.
DR PDBsum; 7MDY; -.
DR AlphaFoldDB; P75958; -.
DR SMR; P75958; -.
DR BioGRID; 4262905; 349.
DR BioGRID; 850037; 5.
DR ComplexPortal; CPX-4262; LolCDE lipoprotein ABC transporter complex.
DR IntAct; P75958; 10.
DR MINT; P75958; -.
DR STRING; 511145.b1118; -.
DR TCDB; 3.A.1.125.1; the atp-binding cassette (abc) superfamily.
DR jPOST; P75958; -.
DR PaxDb; P75958; -.
DR PRIDE; P75958; -.
DR EnsemblBacteria; AAC74202; AAC74202; b1118.
DR EnsemblBacteria; BAA35938; BAA35938; BAA35938.
DR GeneID; 945665; -.
DR KEGG; ecj:JW1104; -.
DR KEGG; eco:b1118; -.
DR PATRIC; fig|511145.12.peg.1164; -.
DR EchoBASE; EB3215; -.
DR eggNOG; COG4591; Bacteria.
DR HOGENOM; CLU_000604_8_1_6; -.
DR InParanoid; P75958; -.
DR OMA; ERTQMIG; -.
DR PhylomeDB; P75958; -.
DR BioCyc; EcoCyc:YCFW-MON; -.
DR BioCyc; MetaCyc:YCFW-MON; -.
DR PRO; PR:P75958; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IPI:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0098797; C:plasma membrane protein complex; IDA:EcoCyc.
DR GO; GO:0140306; F:lipoprotein releasing activity; IDA:EcoCyc.
DR GO; GO:0044874; P:lipoprotein localization to outer membrane; IDA:EcoCyc.
DR GO; GO:0042953; P:lipoprotein transport; IEA:InterPro.
DR InterPro; IPR003838; ABC3_permease_dom.
DR InterPro; IPR011925; LolCE_TM.
DR InterPro; IPR011926; LolE_gammaproteobact.
DR InterPro; IPR025857; MacB_PCD.
DR Pfam; PF02687; FtsX; 1.
DR Pfam; PF12704; MacB_PCD; 1.
DR TIGRFAMs; TIGR02212; lolCE; 1.
DR TIGRFAMs; TIGR02213; lolE_release; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane;
KW Direct protein sequencing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:10783239"
FT CHAIN 2..414
FT /note="Lipoprotein-releasing system transmembrane protein
FT LolE"
FT /id="PRO_0000201818"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TURN 7..12
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 13..16
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 26..58
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 65..69
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:7ARL"
FT STRAND 75..77
FT /evidence="ECO:0007829|PDB:7ARL"
FT HELIX 80..83
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 91..99
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:7ARL"
FT STRAND 106..108
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 115..118
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 120..122
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 129..131
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 132..136
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 147..151
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 152..158
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:7ARI"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 175..177
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 187..189
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 195..198
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 199..202
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 204..207
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 208..214
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 221..229
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 232..239
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 240..242
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 243..245
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 255..258
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 261..267
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 268..270
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 272..283
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 285..297
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 299..308
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 312..343
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 345..356
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 363..365
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 378..394
FT /evidence="ECO:0007829|PDB:7ARJ"
FT HELIX 397..401
FT /evidence="ECO:0007829|PDB:7ARJ"
FT STRAND 403..405
FT /evidence="ECO:0007829|PDB:7ARJ"
FT TURN 407..409
FT /evidence="ECO:0007829|PDB:7ARJ"
SQ SEQUENCE 414 AA; 45344 MW; 37B73A6E5DC58517 CRC64;
MAMPLSLLIG LRFSRGRRRG GMVSLISVIS TIGIALGVAV LIVGLSAMNG FERELNNRIL
AVVPHGEIEA VDQPWTNWQE ALDHVQKVPG IAAAAPYINF TGLVESGANL RAIQVKGVNP
QQEQRLSALP SFVQGDAWRN FKAGEQQIII GKGVADALKV KQGDWVSIMI PNSNPEHKLM
QPKRVRLHVA GILQLSGQLD HSFAMIPLAD AQQYLDMGSS VSGIALKMTD VFNANKLVRD
AGEVTNSYVY IKSWIGTYGY MYRDIQMIRA IMYLAMVLVI GVACFNIVST LVMAVKDKSG
DIAVLRTLGA KDGLIRAIFV WYGLLAGLFG SLCGVIIGVV VSLQLTPIIE WIEKLIGHQF
LSSDIYFIDF LPSELHWLDV FYVLVTALLL SLLASWYPAR RASNIDPARV LSGQ
