ID   LOVHK_BRUA4             Reviewed;         491 AA.
AC   A6X554;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Blue-light-activated histidine kinase;
DE            EC=2.7.13.3;
GN   OrderedLocusNames=Oant_3652;
OS   Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 /
OS   LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37) (Ochrobactrum anthropi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=439375;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC
RC   15819 / NCTC 12168 / Alc 37;
RX   PubMed=21685287; DOI=10.1128/jb.05335-11;
RA   Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M.,
RA   Ugalde R.A., Garcia E., Tolmasky M.E.;
RT   "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic
RT   pathogen and symbiont of several eukaryotic hosts.";
RL   J. Bacteriol. 193:4274-4275(2011).
CC   -!- FUNCTION: Photosensitive kinase that is involved in increased bacterial
CC       virulence upon exposure to light. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- PTM: FMN binds covalently to cysteine after exposure to blue light and
CC       this bond is spontaneously broken in the dark. {ECO:0000250}.
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DR   EMBL; CP000759; ABS16358.1; -; Genomic_DNA.
DR   RefSeq; WP_012093036.1; NC_009668.1.
DR   AlphaFoldDB; A6X554; -.
DR   SMR; A6X554; -.
DR   STRING; 439375.Oant_3652; -.
DR   EnsemblBacteria; ABS16358; ABS16358; Oant_3652.
DR   KEGG; oan:Oant_3652; -.
DR   PATRIC; fig|439375.7.peg.3814; -.
DR   eggNOG; COG2202; Bacteria.
DR   eggNOG; COG3920; Bacteria.
DR   HOGENOM; CLU_000445_114_57_5; -.
DR   OMA; EHRLEFM; -.
DR   OrthoDB; 1755994at2; -.
DR   Proteomes; UP000002301; Chromosome 2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004673; F:protein histidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR   CDD; cd00130; PAS; 2.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR001610; PAC.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR000700; PAS-assoc_C.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013655; PAS_fold_3.
DR   InterPro; IPR011102; Sig_transdc_His_kinase_HWE.
DR   Pfam; PF07536; HWE_HK; 1.
DR   Pfam; PF08447; PAS_3; 1.
DR   Pfam; PF13426; PAS_9; 1.
DR   SMART; SM00911; HWE_HK; 1.
DR   SMART; SM00086; PAC; 2.
DR   SMART; SM00091; PAS; 2.
DR   SUPFAM; SSF55785; SSF55785; 2.
DR   TIGRFAMs; TIGR00229; sensory_box; 2.
DR   PROSITE; PS50113; PAC; 1.
DR   PROSITE; PS50112; PAS; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Chromophore; Flavoprotein; FMN; Kinase; Nucleotide-binding;
KW   Phosphoprotein; Photoreceptor protein; Receptor; Reference proteome;
KW   Repeat; Sensory transduction; Transferase; Virulence.
FT   CHAIN           1..491
FT                   /note="Blue-light-activated histidine kinase"
FT                   /id="PRO_0000361291"
FT   DOMAIN          19..92
FT                   /note="PAS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   DOMAIN          93..147
FT                   /note="PAC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT   DOMAIN          159..230
FT                   /note="PAS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   REGION          286..368
FT                   /note="HWE histidine kinase domain"
FT   MOD_RES         69
FT                   /note="S-4a-FMN cysteine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         289
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   491 AA;  55084 MW;  39AEA33383ED51C7 CRC64;
     MATRVQPSVK VLGASFSQEA NPFTAAVELT PMPMLITNPR LPDNPIIFAN EAFQNLTGYE
     ADEIIGKNCR FLQGPGTDPK HVEIIHSALE AEQSVEIDIL NYKKSGEPFW NRLHISPVKT
     ENGELHHFVS SQLDVTLELG KLVELEKERE TLSIEKQRSS DQLQYIVEVA NVGFWTRDYT
     TGEISCSAEY RRIFGLTPDE PVNYDKIIDM VVLEDRITLI QRSQESFATG KSFRVEYRIT
     NRLGQVRWVE TRAKALLGKS PALLGIVIDV TERKKAEADK ALVTREISHR FKNSMAMVQS
     IANQTLRNAT DPQTANELFS ERLRALSQAH DMLLREDWTG TTISQICETA LAPFNSTFGH
     RIRTSGPELV VSDRVTVSLS LGLYELATNA VKYGALSNEN GTVQFSWDVL ERQGERKFHM
     RWVEDGGPAV ERPTRRGFGQ RLLYSVLTGE LRAKCDINFA PDGLVIDVLA PMTADVFPQL
     DSLSDTQAEP V