LOVHK_PSE14
ID LOVHK_PSE14 Reviewed; 534 AA.
AC Q48IV1;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2009, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Blue-light-activated protein;
DE Includes:
DE RecName: Full=Blue-light-activated histidine kinase;
DE EC=2.7.13.3;
DE Includes:
DE RecName: Full=Response regulator;
GN OrderedLocusNames=PSPPH_2483;
OS Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
OS (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=264730;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1448A / Race 6;
RX PubMed=16159782; DOI=10.1128/jb.187.18.6488-6498.2005;
RA Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R.,
RA Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M.,
RA Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J.,
RA Creasy T., Davidsen T.M., Haft D.H., Zafar N., Zhou L., Halpin R.,
RA Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M.,
RA Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A.,
RA Buell R.;
RT "Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola
RT 1448A reveals divergence among pathovars in genes involved in virulence and
RT transposition.";
RL J. Bacteriol. 187:6488-6498(2005).
CC -!- FUNCTION: Photosensitive kinase and response regulator that is involved
CC in increased bacterial virulence upon exposure to light. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- PTM: FMN binds covalently to cysteine after exposure to blue light and
CC this bond is spontaneously broken in the dark. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAZ33163.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000058; AAZ33163.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_004662206.1; NC_005773.3.
DR AlphaFoldDB; Q48IV1; -.
DR SMR; Q48IV1; -.
DR STRING; 264730.PSPPH_2483; -.
DR EnsemblBacteria; AAZ33163; AAZ33163; PSPPH_2483.
DR KEGG; psp:PSPPH_2483; -.
DR eggNOG; COG0784; Bacteria.
DR eggNOG; COG4191; Bacteria.
DR HOGENOM; CLU_000445_114_51_6; -.
DR OrthoDB; 1755994at2; -.
DR Proteomes; UP000000551; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR CDD; cd00082; HisKA; 1.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF13426; PAS_9; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00086; PAC; 1.
DR SMART; SM00091; PAS; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW ATP-binding; Chromophore; Flavoprotein; FMN; Kinase; Nucleotide-binding;
KW Phosphoprotein; Photoreceptor protein; Receptor; Sensory transduction;
KW Transferase; Virulence.
FT CHAIN 1..534
FT /note="Blue-light-activated protein"
FT /id="PRO_0000361292"
FT DOMAIN 20..93
FT /note="PAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 94..148
FT /note="PAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT DOMAIN 161..390
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT DOMAIN 411..527
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 70
FT /note="S-4a-FMN cysteine"
FT /evidence="ECO:0000250"
FT MOD_RES 164
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 461
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 534 AA; 59110 MW; 7E98AD03CACADEE5 CRC64;
MSEEKARVDN AATGDIQHQG KDIFFAAVET TRMPMIVTDP NRADNPIIFS NRAFLEMTGY
SSEEIIGTNC RFLQGPETDR AVVQSIREAI DERVDISTEI LNYRKDGSTF WNALFISPVY
NDAGDLIYFF ASQLDISRRR DAEDALRQAQ KMEALGQLTG GIAHDFNNLL QVMGGYIDLI
GSAAEKPTID VQRVQRSVHH AKSAVERAST LTKQLLAFAR KQKLQGRVLN LNGLVSSTEP
LIERTFGPHV TIETDLDPAL KNCRIDPTQA EVALLNIFIN ARDALVGRPD PKISIETRNL
VVDELANMSY DGLLPGRYVS IAITDNGIGM PSSIRDRVMD PFFTTKEEGK GSGLGLSMVY
GFAKQSGGAA RIYTEEGVGT TLRLYFPVDE AMLTHAEPPK AVDRRIGSCE RILIVEDRPD
VAELAKMVLD DYGYICEIVL NAREALKKFE SGSRYDLLFT DLIMPGGMNG VMLAREVRRR
YPKIKVLLTT GYAESSIERT DIGGSEFDVV SKPCMPQDLA RKVRQVLDGP NGIA
