LOVHK_PSESM
ID LOVHK_PSESM Reviewed; 534 AA.
AC Q881J7;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Blue-light-activated protein;
DE Includes:
DE RecName: Full=Blue-light-activated histidine kinase;
DE EC=2.7.13.3;
DE AltName: Full=PS-LOV-histidine kinase;
DE Short=PS-LOV-HK;
DE Includes:
DE RecName: Full=Response regulator;
GN OrderedLocusNames=PSPTO_2896;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
RN [2]
RP FUNCTION IN LIGHT SENSING, FLAVIN CHROMOPHORE, KINASE ACTIVITY, INDUCTION,
RP AND ROLE IN VIRULENCE.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=17717187; DOI=10.1126/science.1144306;
RA Swartz T.E., Tseng T.-S., Frederickson M.A., Paris G., Comerci D.J.,
RA Rajashekara G., Kim J.-G., Mudgett M.B., Splitter G.A., Ugalde R.A.,
RA Goldbaum F.A., Briggs W.R., Bogomolni R.A.;
RT "Blue-light-activated histidine kinases: two-component sensors in
RT bacteria.";
RL Science 317:1090-1093(2007).
CC -!- FUNCTION: Photosensitive kinase and response regulator that is involved
CC in increased bacterial virulence upon exposure to light.
CC {ECO:0000269|PubMed:17717187}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- INDUCTION: Constitutively expressed when grown in minimal media.
CC {ECO:0000269|PubMed:17717187}.
CC -!- PTM: FMN binds covalently to cysteine after exposure to blue light and
CC this bond is spontaneously broken in the dark.
CC -!- MISCELLANEOUS: Treatment of phosphorylated protein with alkaline buffer
CC to hydrolyze phospho-aspartates does not significantly reduce
CC phosphorylation in assays performed in vitro. Therefore, it is not
CC clear whether Asp-461 is really phosphorylated by His-164.
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DR EMBL; AE016853; AAO56389.1; -; Genomic_DNA.
DR RefSeq; NP_792694.1; NC_004578.1.
DR RefSeq; WP_007243883.1; NC_004578.1.
DR AlphaFoldDB; Q881J7; -.
DR SMR; Q881J7; -.
DR STRING; 223283.PSPTO_2896; -.
DR EnsemblBacteria; AAO56389; AAO56389; PSPTO_2896.
DR GeneID; 1184550; -.
DR KEGG; pst:PSPTO_2896; -.
DR PATRIC; fig|223283.9.peg.2955; -.
DR eggNOG; COG0784; Bacteria.
DR eggNOG; COG4191; Bacteria.
DR HOGENOM; CLU_000445_114_51_6; -.
DR OMA; HIRIETV; -.
DR OrthoDB; 1755994at2; -.
DR PhylomeDB; Q881J7; -.
DR PHI-base; PHI:3796; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR CDD; cd00082; HisKA; 1.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF13426; PAS_9; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00086; PAC; 1.
DR SMART; SM00091; PAS; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chromophore; Flavoprotein; FMN; Kinase; Nucleotide-binding;
KW Phosphoprotein; Photoreceptor protein; Receptor; Reference proteome;
KW Sensory transduction; Transferase; Virulence.
FT CHAIN 1..534
FT /note="Blue-light-activated protein"
FT /id="PRO_0000361293"
FT DOMAIN 20..93
FT /note="PAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 94..148
FT /note="PAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT DOMAIN 161..390
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT DOMAIN 411..527
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 70
FT /note="S-4a-FMN cysteine"
FT MOD_RES 164
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 461
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 534 AA; 58935 MW; F3D647AA3380F84B CRC64;
MSENKTRVDN AATGDIQHQG KDIFFAAVET TRMPMIVTDP NRPDNPIIFS NRAFLEMTGY
TAEEILGTNC RFLQGPDTDP AVVQSIRDAI AQRNDISAEI INYRKDGSSF WNALFISPVY
NDAGDLIYFF ASQLDISRRK DAEEALRQAQ KMEALGQLTG GIAHDFNNLL QVMGGYIDLI
GSAAEKPVID VQRVQRSVYH AKSAVERAST LTKQLLAFAR KQKLQGRVLN LNGLVSIVEP
LIERTFGPEV AIETDLEPAL KNCRIDPTQA EVALLNIFIN ARDALIGREN PKVFIETRNL
LVDELANMSY DGLLPGRYVS IAVTDNGIGM PASIRDRVMD PFFTTKEEGK GSGLGLSMVY
GFAKQSGGAA RIYTEEGVGT TLRLYFPVDE AGLTNTESPQ ASDRRLGSSE RILIVEDRPD
VAELAKMVLD DYGYVSEIVL NAREALKKFE SGNMYDLLFT DLIMPGGMNG VMLAREVRRR
YPKVKVLLTT GYAESSIERT DIGGSEFDVV SKPCMPHDLA RKVRQVLDGP NGIA
