ID   LPA1_ORYSJ              Reviewed;         714 AA.
AC   B9F4I8; B5SVV3; B5SVV4; B5SVV5; Q0DWE0;
DT   04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=P-loop NTPase domain-containing protein LPA1 {ECO:0000305};
DE   AltName: Full=Protein LOW PHYTIC ACID 1 {ECO:0000303|PubMed:18566795};
DE            Short=OsLpa1 {ECO:0000303|PubMed:18566795};
GN   Name=LPA1 {ECO:0000303|PubMed:18566795};
GN   OrderedLocusNames=Os02g0819400 {ECO:0000312|EMBL:BAF10448.1},
GN   LOC_Os02g57400 {ECO:0000305};
GN   ORFNames=OsJ_08893 {ECO:0000312|EMBL:EEE58056.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, ALTERNATIVE
RP   SPLICING, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Kaybonnet;
RX   PubMed=18566795; DOI=10.1007/s00122-008-0818-z;
RA   Kim S.I., Andaya C.B., Goyal S.S., Tai T.H.;
RT   "The rice OsLpa1 gene encodes a novel protein involved in phytic acid
RT   metabolism.";
RL   Theor. Appl. Genet. 117:769-779(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Required for the accumulation of phytic acid in seeds. Phytic
CC       acid is the primary storage form of phosphorus in cereal grains and
CC       other plant seeds. {ECO:0000269|PubMed:18566795}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1; Synonyms=OsLpa1.1 {ECO:0000303|PubMed:18566795};
CC         IsoId=B9F4I8-1; Sequence=Displayed;
CC       Name=2; Synonyms=OsLpa1.2 {ECO:0000303|PubMed:18566795};
CC         IsoId=B9F4I8-2; Sequence=VSP_057457;
CC       Name=3; Synonyms=OsLpa1.3 {ECO:0000303|PubMed:18566795};
CC         IsoId=B9F4I8-3; Sequence=VSP_057457, VSP_057458, VSP_057459;
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaf blade shoots, leaf sheath
CC       shoots and panicles. {ECO:0000269|PubMed:18566795}.
CC   -!- DISRUPTION PHENOTYPE: Strong reduction in seed phytic acid with a molar
CC       equivalent increase in inorganic phosphate.
CC       {ECO:0000269|PubMed:18566795}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF10448.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; EU366951; ACB38657.1; -; mRNA.
DR   EMBL; EU366953; ACB38659.1; -; mRNA.
DR   EMBL; EU366954; ACB38660.1; -; mRNA.
DR   EMBL; AP008208; BAF10448.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014958; BAS81607.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS81608.1; -; Genomic_DNA.
DR   EMBL; CM000139; EEE58056.1; -; Genomic_DNA.
DR   EMBL; AK100921; BAG94830.1; -; mRNA.
DR   RefSeq; XP_015625422.1; XM_015769936.1. [B9F4I8-1]
DR   AlphaFoldDB; B9F4I8; -.
DR   STRING; 4530.OS02T0819400-01; -.
DR   PaxDb; B9F4I8; -.
DR   PRIDE; B9F4I8; -.
DR   EnsemblPlants; Os02t0819400-01; Os02t0819400-01; Os02g0819400. [B9F4I8-2]
DR   EnsemblPlants; Os02t0819400-03; Os02t0819400-03; Os02g0819400. [B9F4I8-3]
DR   GeneID; 4331161; -.
DR   Gramene; Os02t0819400-01; Os02t0819400-01; Os02g0819400. [B9F4I8-2]
DR   Gramene; Os02t0819400-03; Os02t0819400-03; Os02g0819400. [B9F4I8-3]
DR   KEGG; osa:4331161; -.
DR   eggNOG; ENOG502QR37; Eukaryota.
DR   OrthoDB; 1206241at2759; -.
DR   PlantReactome; R-OSA-1119434; Phytic acid biosynthesis (lipid-independent).
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0010264; P:myo-inositol hexakisphosphate biosynthetic process; IMP:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Reference proteome.
FT   CHAIN           1..714
FT                   /note="P-loop NTPase domain-containing protein LPA1"
FT                   /id="PRO_0000431864"
FT   REGION          1..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          259..293
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          595..689
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        259..287
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        595..618
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        619..641
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        655..673
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..219
FT                   /note="Missing (in isoform 2 and isoform 3)"
FT                   /id="VSP_057457"
FT   VAR_SEQ         587..594
FT                   /note="LMEDDYSV -> VCIGVFTV (in isoform 3)"
FT                   /id="VSP_057458"
FT   VAR_SEQ         595..714
FT                   /note="Missing (in isoform 3)"
FT                   /id="VSP_057459"
SQ   SEQUENCE   714 AA;  78805 MW;  91D77AC4C8200EF3 CRC64;
     MPMPPQCASS KPPSPPPPPH PHEHEVGDDM AEEAPPPPPP PKLLYIAVSD AAARRAFRYT
     RPVLQGTLQL MGCKARHAFK ISKRVFNVMR SEFLDASKSD TADNEENAPS LVKDVEMLKP
     KILEATLSSI PFELYKTQTT IVVSREKFLS VVCDALSSYK YVGPNQKADF LLACRIKERK
     ESVTVLLCGT SGCGKSTLSS LLGSRLGITT VVSTDSIRHM MRGFTDEKQN PLLYASTYHA
     GECLDPVAVA QAKAKRKAQK LDIVSHPNTN EGRDDTSDDK AHHGSSELPP RTELIGSKQM
     AIEGFKAQSE MVIDSLDRLI TSWEEQKQSV IVEGVHLSLN FVMGLMKKHP SIIPFMVYIA
     NEEKHMERFA VRAKYMTLDP AKNRYIKYIR NIRAIQDYLC NRADKHLVPK INNTNVDQSV
     AAIHATVFSC LRRREAGEQL YDLNTNTVAV VNEEYRNQRA ANSLGSKGMF QLIQRQGSSR
     NLMAILNTDG SVTKAWHVDK NNGNGSLDGT SSDKSTKNPM YDTFGKAEPV NLQFGSFGIS
     AWMSDTGGTS HTGSVDDLRA DGIETGGRYY SSCCSSPKVS DCPSKELMED DYSVFGSEED
     ADDPPDAGTD EDLTDEERDM HEIEAGSVDE HSTKSDEEYD DLAMQDVMEN GYWSDDEQAA
     SSTKNSSNQE KNIHGAADGD VVDDEGSGND RFHHNLAFFL KMSKKVAATE LPCA