ID   LPA2_BACSU              Reviewed;          24 AA.
AC   P08497; P94553;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Aspartokinase II operon leader peptide;
DE   AltName: Full=lysC operon attenuator peptide;
GN   Name=ask; Synonyms=aecA; OrderedLocusNames=BSU28480;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3036830; DOI=10.1016/s0021-9258(18)47484-4;
RA   Chen N.-Y., Hu F.M., Paulus H.;
RT   "Nucleotide sequence of the overlapping genes for the subunits of Bacillus
RT   subtilis aspartokinase II and their control regions.";
RL   J. Biol. Chem. 262:8787-8798(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969504; DOI=10.1099/13500872-142-11-3067;
RA   Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA   Emmerson P.T., Harwood C.R.;
RT   "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis
RT   chromosome containing genes responsible for stress responses, the
RT   utilization of plant cell walls and primary metabolism.";
RL   Microbiology 142:3067-3078(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   CHARACTERIZATION, AND MUTAGENESIS OF SER-9; ALA-22 AND HIS-24.
RX   PubMed=1624109; DOI=10.1016/0378-1097(92)90536-w;
RA   Lu Y., Shevtchenko T.N., Paulus H.;
RT   "Fine-structure mapping of cis-acting control sites in the lysC operon of
RT   Bacillus subtilis.";
RL   FEMS Microbiol. Lett. 71:23-27(1992).
CC   -!- FUNCTION: Involved in the repression of the lysC operon which encodes
CC       aspartokinase 2. {ECO:0000269|PubMed:1624109}.
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DR   EMBL; J03294; AAA87317.1; -; Genomic_DNA.
DR   EMBL; Z75208; CAA99579.1; -; Genomic_DNA.
DR   EMBL; AL009126; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; G69590; G69590.
DR   AlphaFoldDB; P08497; -.
DR   Proteomes; UP000001570; Chromosome.
PE   1: Evidence at protein level;
KW   Leader peptide; Reference proteome.
FT   PEPTIDE         1..24
FT                   /note="Aspartokinase II operon leader peptide"
FT                   /id="PRO_0000043973"
FT   MUTAGEN         9
FT                   /note="S->W: Elevated levels of aspartokinase 2."
FT                   /evidence="ECO:0000269|PubMed:1624109"
FT   MUTAGEN         22
FT                   /note="A->G: Elevated levels of aspartokinase 2."
FT                   /evidence="ECO:0000269|PubMed:1624109"
FT   MUTAGEN         24
FT                   /note="H->L: Elevated levels of aspartokinase 2."
FT                   /evidence="ECO:0000269|PubMed:1624109"
SQ   SEQUENCE   24 AA;  2822 MW;  5EA641D7B3FBC916 CRC64;
     MKKAERGASP KQIKPHRYYL LAVH