ID   LPAAT_ORYSJ             Reviewed;         414 AA.
AC   Q0J0A4; Q650U5;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-SEP-2014, sequence version 3.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Probable 1-acylglycerol-3-phosphate O-acyltransferase;
DE            EC=2.3.1.51;
DE   AltName: Full=Lipid droplet-binding protein CGI-58 homolog;
GN   OrderedLocusNames=Os09g0520200, LOC_Os09g34860; ORFNames=P0669G04.7;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Lysophosphatidic acid acyltransferase which functions in
CC       phosphatidic acid biosynthesis. May regulate neutral lipid accumulation
CC       and participate in the regulation of lipid turnover in vegetative
CC       cells. May possess additional triacylglycerol lipase and phospholipase
CC       A2 activities in vitro (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-
CC         sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:19709,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342,
CC         ChEBI:CHEBI:58608; EC=2.3.1.51;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC       may constitute the binding site for the phosphate moiety of the
CC       glycerol-3-phosphate. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD46672.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF25611.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP006175; BAD46672.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008215; BAF25611.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_015651253.1; XM_015795767.1.
DR   AlphaFoldDB; Q0J0A4; -.
DR   SMR; Q0J0A4; -.
DR   STRING; 4530.OS09T0520200-00; -.
DR   ESTHER; orysa-Q0J0A4; CGI-58_ABHD5_ABHD4.
DR   PaxDb; Q0J0A4; -.
DR   PRIDE; Q0J0A4; -.
DR   GeneID; 4347605; -.
DR   KEGG; osa:4347605; -.
DR   eggNOG; KOG4409; Eukaryota.
DR   InParanoid; Q0J0A4; -.
DR   OrthoDB; 1555935at2759; -.
DR   Proteomes; UP000000763; Chromosome 9.
DR   Proteomes; UP000059680; Chromosome 9.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003841; F:1-acylglycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IBA:GO_Central.
DR   GO; GO:0042171; F:lysophosphatidic acid acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0004623; F:phospholipase A2 activity; IBA:GO_Central.
DR   GO; GO:0055088; P:lipid homeostasis; IBA:GO_Central.
DR   GO; GO:0006654; P:phosphatidic acid biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; Lipid biosynthesis; Lipid metabolism;
KW   Phospholipid biosynthesis; Phospholipid metabolism; Reference proteome;
KW   Transferase.
FT   CHAIN           1..414
FT                   /note="Probable 1-acylglycerol-3-phosphate O-
FT                   acyltransferase"
FT                   /id="PRO_0000430170"
FT   DOMAIN          117..382
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           193..197
FT                   /note="GXSXG"
FT   MOTIF           375..380
FT                   /note="HXXXXD motif"
SQ   SEQUENCE   414 AA;  45728 MW;  25DC41C01A97FB2C CRC64;
     MRRAAAAAVT VTTTTRMAAE GMSTAAAAAE ATATAAPAAG SRWGRAWPSA LRWIPTSTDR
     IIAAEKRLLS IVKTGYVQEQ VNIGSSPPGS KVRWFRSSSD EPRFINTVTF DSEENAPTLV
     MVHGYGASQG FFFRNFDALA SRFRVIAIDQ LGWGGSSRPD FTCKSTEETE AWFIDSFEEW
     RKAKNLSNFI LLGHSFGGYV AAKYALQHPE HVQHLILVGP AGFSSETEHS SEWLTKFRAT
     WKGMLVNHLW ESNFTPQRIV RGLGPWGPGL VQRYTSARFG SHSTGELLTE QESTLLTDYI
     YHTLAAKASG ELCLKHIFSF GAFVRKPLLQ SASDWKVPTT FIYGQQDWMN YQGAQQARKE
     MKVPCEIIRV PQGGHFVFID NPSGFHSAVF HACRKFLSGD GEEGLSLPEG LTSA