LPAR4_HUMAN
ID LPAR4_HUMAN Reviewed; 370 AA.
AC Q99677; B2RAC7; O15132; Q502U9; Q6NSP5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Lysophosphatidic acid receptor 4;
DE Short=LPA receptor 4;
DE Short=LPA-4;
DE AltName: Full=G-protein coupled receptor 23;
DE AltName: Full=P2Y purinoceptor 9;
DE Short=P2Y9;
DE AltName: Full=P2Y5-like receptor;
DE AltName: Full=Purinergic receptor 9;
GN Name=LPAR4; Synonyms=GPR23, LPA4, P2RY9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9073069; DOI=10.1016/s0378-1119(96)00722-6;
RA O'Dowd B.F., Nguyen T., Jung B.P., Marchese A., Cheng R., Heng H.H.Q.,
RA Kolakowski L.F. Jr., Lynch K.R., George S.R.;
RT "Cloning and chromosomal mapping of four putative novel human G-protein-
RT coupled receptor genes.";
RL Gene 187:75-81(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Bohm S.K., Khitin L.M., Payan D.P., Bunnett N.W.;
RT "Cloning, characterization and chromosomal localization of a novel human
RT purinergic receptor (P2Y9).";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9223435; DOI=10.1006/bbrc.1997.6895;
RA Janssens R., Boeynaems J.-M., Godart M., Communi D.;
RT "Cloning of a human heptahelical receptor closely related to the P2Y5
RT receptor.";
RL Biochem. Biophys. Res. Commun. 236:106-112(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kopatz S.A., Aronstam R.S., Sharma S.V.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12724320; DOI=10.1074/jbc.m302648200;
RA Noguchi K., Ishii S., Shimizu T.;
RT "Identification of p2y9/GPR23 as a novel G protein-coupled receptor for
RT lysophosphatidic acid, structurally distant from the Edg family.";
RL J. Biol. Chem. 278:25600-25606(2003).
RN [10]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-183.
RC TISSUE=Plasma;
RX PubMed=16335952; DOI=10.1021/pr0502065;
RA Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA Smith R.D.;
RT "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT hydrazide chemistry, and mass spectrometry.";
RL J. Proteome Res. 4:2070-2080(2005).
CC -!- FUNCTION: Receptor for lysophosphatidic acid (LPA), a mediator of
CC diverse cellular activities. Transduces a signal by increasing the
CC intracellular calcium ions and by stimulating adenylyl cyclase
CC activity. The rank order of potency for agonists of this receptor is 1-
CC oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-
CC alkenyl-LPA. {ECO:0000269|PubMed:12724320}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: High expression in ovary. Not detected in the brain
CC regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus
CC and hippocampus. {ECO:0000269|PubMed:12724320}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U66578; AAC51301.1; -; Genomic_DNA.
DR EMBL; U90323; AAB62087.1; -; Genomic_DNA.
DR EMBL; U90322; AAB62088.1; -; mRNA.
DR EMBL; AF005419; AAB66322.1; -; Genomic_DNA.
DR EMBL; AY301274; AAP58404.1; -; Genomic_DNA.
DR EMBL; AK314134; BAG36824.1; -; mRNA.
DR EMBL; AL590083; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471104; EAW98596.1; -; Genomic_DNA.
DR EMBL; BC069996; AAH69996.1; -; mRNA.
DR EMBL; BC074722; AAH74722.1; -; mRNA.
DR EMBL; BC095538; AAH95538.1; -; mRNA.
DR CCDS; CCDS14441.1; -.
DR PIR; JC5549; JC5549.
DR RefSeq; NP_001264929.1; NM_001278000.1.
DR RefSeq; NP_005287.1; NM_005296.2.
DR RefSeq; XP_005262183.1; XM_005262126.3.
DR RefSeq; XP_006724702.1; XM_006724639.3.
DR RefSeq; XP_016884926.1; XM_017029437.1.
DR RefSeq; XP_016884927.1; XM_017029438.1.
DR AlphaFoldDB; Q99677; -.
DR SMR; Q99677; -.
DR BioGRID; 109105; 85.
DR IntAct; Q99677; 32.
DR STRING; 9606.ENSP00000408205; -.
DR BindingDB; Q99677; -.
DR ChEMBL; CHEMBL5968; -.
DR DrugBank; DB01069; Promethazine.
DR GuidetoPHARMACOLOGY; 94; -.
DR SwissLipids; SLP:000001577; -.
DR TCDB; 9.A.14.13.9; the g-protein-coupled receptor (gpcr) family.
DR GlyGen; Q99677; 4 sites.
DR iPTMnet; Q99677; -.
DR PhosphoSitePlus; Q99677; -.
DR BioMuta; LPAR4; -.
DR DMDM; 2495042; -.
DR PaxDb; Q99677; -.
DR PeptideAtlas; Q99677; -.
DR PRIDE; Q99677; -.
DR Antibodypedia; 35263; 316 antibodies from 30 providers.
DR DNASU; 2846; -.
DR Ensembl; ENST00000435339.3; ENSP00000408205.2; ENSG00000147145.14.
DR Ensembl; ENST00000614823.5; ENSP00000478261.1; ENSG00000147145.14.
DR GeneID; 2846; -.
DR KEGG; hsa:2846; -.
DR MANE-Select; ENST00000614823.5; ENSP00000478261.1; NM_001278000.3; NP_001264929.1.
DR UCSC; uc010nme.4; human.
DR CTD; 2846; -.
DR DisGeNET; 2846; -.
DR GeneCards; LPAR4; -.
DR HGNC; HGNC:4478; LPAR4.
DR HPA; ENSG00000147145; Tissue enhanced (brain, ovary).
DR MalaCards; LPAR4; -.
DR MIM; 300086; gene.
DR neXtProt; NX_Q99677; -.
DR OpenTargets; ENSG00000147145; -.
DR Orphanet; 146; Differentiated thyroid carcinoma.
DR PharmGKB; PA162394222; -.
DR VEuPathDB; HostDB:ENSG00000147145; -.
DR eggNOG; ENOG502QV8W; Eukaryota.
DR GeneTree; ENSGT01040000240444; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; Q99677; -.
DR OMA; TVGHGCD; -.
DR OrthoDB; 706068at2759; -.
DR PhylomeDB; Q99677; -.
DR TreeFam; TF350009; -.
DR PathwayCommons; Q99677; -.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR Reactome; R-HSA-417957; P2Y receptors.
DR SignaLink; Q99677; -.
DR SIGNOR; Q99677; -.
DR BioGRID-ORCS; 2846; 6 hits in 692 CRISPR screens.
DR GeneWiki; LPAR4; -.
DR GenomeRNAi; 2846; -.
DR Pharos; Q99677; Tchem.
DR PRO; PR:Q99677; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q99677; protein.
DR Bgee; ENSG00000147145; Expressed in sural nerve and 112 other tissues.
DR ExpressionAtlas; Q99677; baseline and differential.
DR Genevisible; Q99677; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0016604; C:nuclear body; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0035727; F:lysophosphatidic acid binding; IEA:Ensembl.
DR GO; GO:0070915; F:lysophosphatidic acid receptor activity; IBA:GO_Central.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IBA:GO_Central.
DR GO; GO:0035025; P:positive regulation of Rho protein signal transduction; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Lipid-binding; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..370
FT /note="Lysophosphatidic acid receptor 4"
FT /id="PRO_0000070033"
FT TOPO_DOM 1..43
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..73
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..112
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..203
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..294
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 316..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 15
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 28
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:16335952"
FT DISULFID 111..188
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 49
FT /note="V -> A (in Ref. 8; AAH69996)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="F -> L (in Ref. 3; AAB66322)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="I -> V (in Ref. 8; AAH95538)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 41895 MW; 20857F52A3929E48 CRC64;
MGDRRFIDFQ FQDSNSSLRP RLGNATANNT CIVDDSFKYN LNGAVYSVVF ILGLITNSVS
LFVFCFRMKM RSETAIFITN LAVSDLLFVC TLPFKIFYNF NRHWPFGDTL CKISGTAFLT
NIYGSMLFLT CISVDRFLAI VYPFRSRTIR TRRNSAIVCA GVWILVLSGG ISASLFSTTN
VNNATTTCFE GFSKRVWKTY LSKITIFIEV VGFIIPLILN VSCSSVVLRT LRKPATLSQI
GTNKKKVLKM ITVHMAVFVV CFVPYNSVLF LYALVRSQAI TNCFLERFAK IMYPITLCLA
TLNCCFDPFI YYFTLESFQK SFYINAHIRM ESLFKTETPL TTKPSLPAIQ EEVSDQTTNN
GGELMLESTF
