ID   LPE1_MAIZE              Reviewed;         527 AA.
AC   Q41760;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Nucleobase-ascorbate transporter LPE1;
DE   AltName: Full=Leaf permease protein 1;
GN   Name=LPE1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC   TISSUE=Leaf;
RX   PubMed=8721750; DOI=10.2307/3870325;
RA   Schultes N.P., Brutnell T.P., Allen A., Dellaporta S.L., Nelson T.,
RA   Chen J.;
RT   "Leaf permease1 gene of maize is required for chloroplast development.";
RL   Plant Cell 8:463-475(1996).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=11283348; DOI=10.2307/3871352;
RA   Argyrou E., Sophianopoulou V., Schultes N.P., Diallinas G.;
RT   "Functional characterization of a maize purine transporter by expression in
RT   Aspergillus nidulans.";
RL   Plant Cell 13:953-964(2001).
CC   -!- FUNCTION: High affinity uric acid-xanthine transporter in A.nidulans.
CC       Binds, but cannot transport ascorbic acid.
CC       {ECO:0000269|PubMed:11283348}.
CC   -!- ACTIVITY REGULATION: Inhibited by excess of xanthin, uric acid and
CC       ascorbic acid, and by 100 um N,N-dicyclohexylcarbodiimide and 30 um
CC       carbonyl cyanide m-chlorophenyl-hydrazone.
CC       {ECO:0000269|PubMed:11283348}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=30 uM for xanthine {ECO:0000269|PubMed:11283348};
CC         Note=Measured in the absence of ascorbic acid.;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in roots.
CC       {ECO:0000269|PubMed:8721750}.
CC   -!- DISRUPTION PHENOTYPE: Disrupted chloroplast ultrastructure.
CC       {ECO:0000269|PubMed:8721750}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. {ECO:0000305}.
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DR   EMBL; U43034; AAB17501.2; -; mRNA.
DR   PIR; T02719; T02719.
DR   RefSeq; NP_001105384.1; NM_001111914.1.
DR   AlphaFoldDB; Q41760; -.
DR   SMR; Q41760; -.
DR   STRING; 4577.GRMZM5G858417_P01; -.
DR   TCDB; 2.A.40.6.3; the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.
DR   PaxDb; Q41760; -.
DR   GeneID; 542332; -.
DR   KEGG; zma:542332; -.
DR   eggNOG; KOG1292; Eukaryota.
DR   OrthoDB; 387031at2759; -.
DR   BioCyc; MetaCyc:MON-14782; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; Q41760; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR   InterPro; IPR006043; NCS2.
DR   Pfam; PF00860; Xan_ur_permease; 1.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..527
FT                   /note="Nucleobase-ascorbate transporter LPE1"
FT                   /id="PRO_0000270170"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        361..383
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        387..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        427..447
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        458..478
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   527 AA;  58122 MW;  5A6208081DDDFA7A CRC64;
     MPPVKAEDLV VHAVKEQFAG LDYCITSPPP WITTVLVGFQ HYLVMLGTTV LIATIIVPLM
     GGGHAEKAIV IQTILFLSGI NTLLQVHFGT RLPAVMSGSY TYIYPAVAII LSPRYALLID
     PLERFVFTMR SLQGALIIAG VFQAVVGFFG IWRVFIRFLS PLAAVPFVTL TGLGLFFFAF
     PGVTKCIEVG LPALVLLVIF AEYASHLFAK GSFVFSRCAV LVTVVIIWIY AEILTAAGAY
     NERGPVTQFS CRADRSGIIQ GSPWVRFPYP FQWGYPIFCF QDCFAMLAAS FASLIESTGT
     LIAVSRYSGA TFCPPSVFSR GIGWEGISII LDGMCGTLTG TAASVENAGL LAVTRVGSRR
     VIKISALFMI FFSLFAKFGA VLASIPLPIF AALYCVLFAY SAGAGFSLLQ YCNLNSLRTK
     FILSISLFLG LSIPQYFRVY EMFFGFGPVH THSVAFNVMV NVIFSSPATV AAILAYLLDC
     THLYWEASVK KDRGWFWWEK FKSYKYDGRS EEFYRLPYGL SRYFPSL